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Complex dissemination of the diversified mcr-1-harbouring plasmids in Escherichia coli of different sequence types
The emergence of the mobilized colistin resistance gene, representing a novel mechanism for bacterial drug resistance, challenges the last resort against the severe infections by Gram-negative bacteria with multi-drug resistances. Very recently, we showed the diversity in the mcr-1-carrying plasmid...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5347679/ https://www.ncbi.nlm.nih.gov/pubmed/27741523 http://dx.doi.org/10.18632/oncotarget.12621 |
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author | Wang, Qingjing Li, Zhencui Lin, Jingxia Wang, Xiuna Deng, Xianbo Feng, Youjun |
author_facet | Wang, Qingjing Li, Zhencui Lin, Jingxia Wang, Xiuna Deng, Xianbo Feng, Youjun |
author_sort | Wang, Qingjing |
collection | PubMed |
description | The emergence of the mobilized colistin resistance gene, representing a novel mechanism for bacterial drug resistance, challenges the last resort against the severe infections by Gram-negative bacteria with multi-drug resistances. Very recently, we showed the diversity in the mcr-1-carrying plasmid reservoirs from the gut microbiota. Here, we reported that a similar but more complex scenario is present in the healthy swine populations, Southern China, 2016. Amongst the 1026 pieces of Escherichia coli isolates from 3 different pig farms, 302 E. coli isolates were determined to be positive for the mcr-1 gene (30%, 302/1026). Multi-locus sequence typing assigned no less than 11 kinds of sequence types including one novel Sequence Type to these mcr-1-positive strains. PCR analyses combined with the direct DNA sequencing revealed unexpected complexity of the mcr-1-harbouring plasmids whose backbones are at least grouped into 6 types four of which are new. Transcriptional analyses showed that the mcr-1 promoter of different origins exhibits similar activity. It seems likely that complex dissemination of the diversified mcr-1-bearing plasmids occurs amongst the various ST E. coli inhabiting the healthy swine populations, in Southern China. |
format | Online Article Text |
id | pubmed-5347679 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-53476792017-03-31 Complex dissemination of the diversified mcr-1-harbouring plasmids in Escherichia coli of different sequence types Wang, Qingjing Li, Zhencui Lin, Jingxia Wang, Xiuna Deng, Xianbo Feng, Youjun Oncotarget Research Paper: Immunology The emergence of the mobilized colistin resistance gene, representing a novel mechanism for bacterial drug resistance, challenges the last resort against the severe infections by Gram-negative bacteria with multi-drug resistances. Very recently, we showed the diversity in the mcr-1-carrying plasmid reservoirs from the gut microbiota. Here, we reported that a similar but more complex scenario is present in the healthy swine populations, Southern China, 2016. Amongst the 1026 pieces of Escherichia coli isolates from 3 different pig farms, 302 E. coli isolates were determined to be positive for the mcr-1 gene (30%, 302/1026). Multi-locus sequence typing assigned no less than 11 kinds of sequence types including one novel Sequence Type to these mcr-1-positive strains. PCR analyses combined with the direct DNA sequencing revealed unexpected complexity of the mcr-1-harbouring plasmids whose backbones are at least grouped into 6 types four of which are new. Transcriptional analyses showed that the mcr-1 promoter of different origins exhibits similar activity. It seems likely that complex dissemination of the diversified mcr-1-bearing plasmids occurs amongst the various ST E. coli inhabiting the healthy swine populations, in Southern China. Impact Journals LLC 2016-10-12 /pmc/articles/PMC5347679/ /pubmed/27741523 http://dx.doi.org/10.18632/oncotarget.12621 Text en Copyright: © 2016 Wang et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper: Immunology Wang, Qingjing Li, Zhencui Lin, Jingxia Wang, Xiuna Deng, Xianbo Feng, Youjun Complex dissemination of the diversified mcr-1-harbouring plasmids in Escherichia coli of different sequence types |
title | Complex dissemination of the diversified mcr-1-harbouring plasmids in Escherichia coli of different sequence types |
title_full | Complex dissemination of the diversified mcr-1-harbouring plasmids in Escherichia coli of different sequence types |
title_fullStr | Complex dissemination of the diversified mcr-1-harbouring plasmids in Escherichia coli of different sequence types |
title_full_unstemmed | Complex dissemination of the diversified mcr-1-harbouring plasmids in Escherichia coli of different sequence types |
title_short | Complex dissemination of the diversified mcr-1-harbouring plasmids in Escherichia coli of different sequence types |
title_sort | complex dissemination of the diversified mcr-1-harbouring plasmids in escherichia coli of different sequence types |
topic | Research Paper: Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5347679/ https://www.ncbi.nlm.nih.gov/pubmed/27741523 http://dx.doi.org/10.18632/oncotarget.12621 |
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