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Complex dissemination of the diversified mcr-1-harbouring plasmids in Escherichia coli of different sequence types

The emergence of the mobilized colistin resistance gene, representing a novel mechanism for bacterial drug resistance, challenges the last resort against the severe infections by Gram-negative bacteria with multi-drug resistances. Very recently, we showed the diversity in the mcr-1-carrying plasmid...

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Autores principales: Wang, Qingjing, Li, Zhencui, Lin, Jingxia, Wang, Xiuna, Deng, Xianbo, Feng, Youjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5347679/
https://www.ncbi.nlm.nih.gov/pubmed/27741523
http://dx.doi.org/10.18632/oncotarget.12621
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author Wang, Qingjing
Li, Zhencui
Lin, Jingxia
Wang, Xiuna
Deng, Xianbo
Feng, Youjun
author_facet Wang, Qingjing
Li, Zhencui
Lin, Jingxia
Wang, Xiuna
Deng, Xianbo
Feng, Youjun
author_sort Wang, Qingjing
collection PubMed
description The emergence of the mobilized colistin resistance gene, representing a novel mechanism for bacterial drug resistance, challenges the last resort against the severe infections by Gram-negative bacteria with multi-drug resistances. Very recently, we showed the diversity in the mcr-1-carrying plasmid reservoirs from the gut microbiota. Here, we reported that a similar but more complex scenario is present in the healthy swine populations, Southern China, 2016. Amongst the 1026 pieces of Escherichia coli isolates from 3 different pig farms, 302 E. coli isolates were determined to be positive for the mcr-1 gene (30%, 302/1026). Multi-locus sequence typing assigned no less than 11 kinds of sequence types including one novel Sequence Type to these mcr-1-positive strains. PCR analyses combined with the direct DNA sequencing revealed unexpected complexity of the mcr-1-harbouring plasmids whose backbones are at least grouped into 6 types four of which are new. Transcriptional analyses showed that the mcr-1 promoter of different origins exhibits similar activity. It seems likely that complex dissemination of the diversified mcr-1-bearing plasmids occurs amongst the various ST E. coli inhabiting the healthy swine populations, in Southern China.
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spelling pubmed-53476792017-03-31 Complex dissemination of the diversified mcr-1-harbouring plasmids in Escherichia coli of different sequence types Wang, Qingjing Li, Zhencui Lin, Jingxia Wang, Xiuna Deng, Xianbo Feng, Youjun Oncotarget Research Paper: Immunology The emergence of the mobilized colistin resistance gene, representing a novel mechanism for bacterial drug resistance, challenges the last resort against the severe infections by Gram-negative bacteria with multi-drug resistances. Very recently, we showed the diversity in the mcr-1-carrying plasmid reservoirs from the gut microbiota. Here, we reported that a similar but more complex scenario is present in the healthy swine populations, Southern China, 2016. Amongst the 1026 pieces of Escherichia coli isolates from 3 different pig farms, 302 E. coli isolates were determined to be positive for the mcr-1 gene (30%, 302/1026). Multi-locus sequence typing assigned no less than 11 kinds of sequence types including one novel Sequence Type to these mcr-1-positive strains. PCR analyses combined with the direct DNA sequencing revealed unexpected complexity of the mcr-1-harbouring plasmids whose backbones are at least grouped into 6 types four of which are new. Transcriptional analyses showed that the mcr-1 promoter of different origins exhibits similar activity. It seems likely that complex dissemination of the diversified mcr-1-bearing plasmids occurs amongst the various ST E. coli inhabiting the healthy swine populations, in Southern China. Impact Journals LLC 2016-10-12 /pmc/articles/PMC5347679/ /pubmed/27741523 http://dx.doi.org/10.18632/oncotarget.12621 Text en Copyright: © 2016 Wang et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper: Immunology
Wang, Qingjing
Li, Zhencui
Lin, Jingxia
Wang, Xiuna
Deng, Xianbo
Feng, Youjun
Complex dissemination of the diversified mcr-1-harbouring plasmids in Escherichia coli of different sequence types
title Complex dissemination of the diversified mcr-1-harbouring plasmids in Escherichia coli of different sequence types
title_full Complex dissemination of the diversified mcr-1-harbouring plasmids in Escherichia coli of different sequence types
title_fullStr Complex dissemination of the diversified mcr-1-harbouring plasmids in Escherichia coli of different sequence types
title_full_unstemmed Complex dissemination of the diversified mcr-1-harbouring plasmids in Escherichia coli of different sequence types
title_short Complex dissemination of the diversified mcr-1-harbouring plasmids in Escherichia coli of different sequence types
title_sort complex dissemination of the diversified mcr-1-harbouring plasmids in escherichia coli of different sequence types
topic Research Paper: Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5347679/
https://www.ncbi.nlm.nih.gov/pubmed/27741523
http://dx.doi.org/10.18632/oncotarget.12621
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