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Babao Dan attenuates hepatic fibrosis by inhibiting hepatic stellate cells activation and proliferation via TLR4 signaling pathway

Babao Dan (BBD), a traditional Chinese medicine, has been widely used as a complementary and alternative medicine to treat chronic liver diseases. In this study, we aimed to observe the protective effect of BBD on rat hepatic fibrosis induced by diethylnitrosamine (DEN) and explore it possible mecha...

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Autores principales: Liang, Lei, Yang, Xue, Yu, Yang, Li, Xiaoyong, Wu, Yechen, Shi, Rongyu, Jiang, Jinghua, Gao, Lu, Ye, Fei, Zhao, Qiudong, Li, Rong, Wei, Lixin, Han, Zhipeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5347713/
https://www.ncbi.nlm.nih.gov/pubmed/27776340
http://dx.doi.org/10.18632/oncotarget.12783
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author Liang, Lei
Yang, Xue
Yu, Yang
Li, Xiaoyong
Wu, Yechen
Shi, Rongyu
Jiang, Jinghua
Gao, Lu
Ye, Fei
Zhao, Qiudong
Li, Rong
Wei, Lixin
Han, Zhipeng
author_facet Liang, Lei
Yang, Xue
Yu, Yang
Li, Xiaoyong
Wu, Yechen
Shi, Rongyu
Jiang, Jinghua
Gao, Lu
Ye, Fei
Zhao, Qiudong
Li, Rong
Wei, Lixin
Han, Zhipeng
author_sort Liang, Lei
collection PubMed
description Babao Dan (BBD), a traditional Chinese medicine, has been widely used as a complementary and alternative medicine to treat chronic liver diseases. In this study, we aimed to observe the protective effect of BBD on rat hepatic fibrosis induced by diethylnitrosamine (DEN) and explore it possible mechanism. BBD was administrated while DEN was given. After eight weeks, values of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) indicated that BBD significantly protected liver from damaging by DEN and had no obvious side effect on normal rat livers. Meanwhile, BBD attenuated hepatic inflammation and fibrosis in DEN-induced rat livers through histopathological examination and hepatic hydroxyproline content. Furthermore, we found that BBD inhibited hepatic stellate cells activation and proliferation without altering the concentration of lipopolysaccharide (LPS) in portal vein. In vitro study, serum from BBD treated rats (BBD-serum) could also significantly suppress LPS-induced HSCs activation through TLR4/NF-κB pathway. In addition, BBD-serum also inhibited the proliferation of HSCs by regulating TLR4/ERK pathway. Our study demonstrated that BBD may provide a new therapy strategy of hepatic injury and hepatic fibrosis.
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spelling pubmed-53477132017-03-31 Babao Dan attenuates hepatic fibrosis by inhibiting hepatic stellate cells activation and proliferation via TLR4 signaling pathway Liang, Lei Yang, Xue Yu, Yang Li, Xiaoyong Wu, Yechen Shi, Rongyu Jiang, Jinghua Gao, Lu Ye, Fei Zhao, Qiudong Li, Rong Wei, Lixin Han, Zhipeng Oncotarget Research Paper Babao Dan (BBD), a traditional Chinese medicine, has been widely used as a complementary and alternative medicine to treat chronic liver diseases. In this study, we aimed to observe the protective effect of BBD on rat hepatic fibrosis induced by diethylnitrosamine (DEN) and explore it possible mechanism. BBD was administrated while DEN was given. After eight weeks, values of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) indicated that BBD significantly protected liver from damaging by DEN and had no obvious side effect on normal rat livers. Meanwhile, BBD attenuated hepatic inflammation and fibrosis in DEN-induced rat livers through histopathological examination and hepatic hydroxyproline content. Furthermore, we found that BBD inhibited hepatic stellate cells activation and proliferation without altering the concentration of lipopolysaccharide (LPS) in portal vein. In vitro study, serum from BBD treated rats (BBD-serum) could also significantly suppress LPS-induced HSCs activation through TLR4/NF-κB pathway. In addition, BBD-serum also inhibited the proliferation of HSCs by regulating TLR4/ERK pathway. Our study demonstrated that BBD may provide a new therapy strategy of hepatic injury and hepatic fibrosis. Impact Journals LLC 2016-10-20 /pmc/articles/PMC5347713/ /pubmed/27776340 http://dx.doi.org/10.18632/oncotarget.12783 Text en Copyright: © 2016 Liang et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Liang, Lei
Yang, Xue
Yu, Yang
Li, Xiaoyong
Wu, Yechen
Shi, Rongyu
Jiang, Jinghua
Gao, Lu
Ye, Fei
Zhao, Qiudong
Li, Rong
Wei, Lixin
Han, Zhipeng
Babao Dan attenuates hepatic fibrosis by inhibiting hepatic stellate cells activation and proliferation via TLR4 signaling pathway
title Babao Dan attenuates hepatic fibrosis by inhibiting hepatic stellate cells activation and proliferation via TLR4 signaling pathway
title_full Babao Dan attenuates hepatic fibrosis by inhibiting hepatic stellate cells activation and proliferation via TLR4 signaling pathway
title_fullStr Babao Dan attenuates hepatic fibrosis by inhibiting hepatic stellate cells activation and proliferation via TLR4 signaling pathway
title_full_unstemmed Babao Dan attenuates hepatic fibrosis by inhibiting hepatic stellate cells activation and proliferation via TLR4 signaling pathway
title_short Babao Dan attenuates hepatic fibrosis by inhibiting hepatic stellate cells activation and proliferation via TLR4 signaling pathway
title_sort babao dan attenuates hepatic fibrosis by inhibiting hepatic stellate cells activation and proliferation via tlr4 signaling pathway
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5347713/
https://www.ncbi.nlm.nih.gov/pubmed/27776340
http://dx.doi.org/10.18632/oncotarget.12783
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