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The role of transcriptional coactivator TAZ in gliomas
The transcriptional coactivator with PDZ-binding motif (TAZ) is one of the important downstream effectors of Hippo pathway. In this study, the potential implication of TAZ in gliomagenesis was explored. TAZ expression was identified to be upregulated in glioma specimens and positively correlated wit...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5347724/ https://www.ncbi.nlm.nih.gov/pubmed/27764783 http://dx.doi.org/10.18632/oncotarget.12625 |
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author | Li, Weijie Dong, Shicai Wei, Wei Wang, Guangxiu Zhang, Anling Pu, Peiyu Jia, Zhifan |
author_facet | Li, Weijie Dong, Shicai Wei, Wei Wang, Guangxiu Zhang, Anling Pu, Peiyu Jia, Zhifan |
author_sort | Li, Weijie |
collection | PubMed |
description | The transcriptional coactivator with PDZ-binding motif (TAZ) is one of the important downstream effectors of Hippo pathway. In this study, the potential implication of TAZ in gliomagenesis was explored. TAZ expression was identified to be upregulated in glioma specimens and positively correlated with tumor grade. Meanwhile, its expression in nucleus was increased more significantly with the ascending order of tumor grade. Knocking down TAZ inhibited glioma cell proliferation, invasion and promoted apoptosis. Conversely, enforced upregulation of TAZ promoted proliferation, invasion of glioma cells, and suppressed apoptosis in vitro. When orthotopic glioblastoma mouse model implanted with TAZ knocked down cells, glioma growth was inhibited and survival period was prolonged. Expression of Ki67, MMP-9, Cyclin D1, Bcl-2 and C-myc was varied in accordance with the level of TAZ in glioma cell. The biomarkers of EMT (epithelial-mesenchymal transition), vimentin and N-cadherin, were downregulated when TAZ was suppressed. Using Co-immunoprecipitation TAZ was identified to bind to TEAD4. Therefore, our findings indicate that TAZ is overexpressed in glioma and translocated more into nucleus in high grade glioma. TAZ is involved in gliomagenesis by promoting glioma growth and may benefit to EMT progression. This result suggests that TAZ serves as a potential target for the treatment of glioma. |
format | Online Article Text |
id | pubmed-5347724 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-53477242017-03-31 The role of transcriptional coactivator TAZ in gliomas Li, Weijie Dong, Shicai Wei, Wei Wang, Guangxiu Zhang, Anling Pu, Peiyu Jia, Zhifan Oncotarget Research Paper The transcriptional coactivator with PDZ-binding motif (TAZ) is one of the important downstream effectors of Hippo pathway. In this study, the potential implication of TAZ in gliomagenesis was explored. TAZ expression was identified to be upregulated in glioma specimens and positively correlated with tumor grade. Meanwhile, its expression in nucleus was increased more significantly with the ascending order of tumor grade. Knocking down TAZ inhibited glioma cell proliferation, invasion and promoted apoptosis. Conversely, enforced upregulation of TAZ promoted proliferation, invasion of glioma cells, and suppressed apoptosis in vitro. When orthotopic glioblastoma mouse model implanted with TAZ knocked down cells, glioma growth was inhibited and survival period was prolonged. Expression of Ki67, MMP-9, Cyclin D1, Bcl-2 and C-myc was varied in accordance with the level of TAZ in glioma cell. The biomarkers of EMT (epithelial-mesenchymal transition), vimentin and N-cadherin, were downregulated when TAZ was suppressed. Using Co-immunoprecipitation TAZ was identified to bind to TEAD4. Therefore, our findings indicate that TAZ is overexpressed in glioma and translocated more into nucleus in high grade glioma. TAZ is involved in gliomagenesis by promoting glioma growth and may benefit to EMT progression. This result suggests that TAZ serves as a potential target for the treatment of glioma. Impact Journals LLC 2016-10-13 /pmc/articles/PMC5347724/ /pubmed/27764783 http://dx.doi.org/10.18632/oncotarget.12625 Text en Copyright: © 2016 Li et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Li, Weijie Dong, Shicai Wei, Wei Wang, Guangxiu Zhang, Anling Pu, Peiyu Jia, Zhifan The role of transcriptional coactivator TAZ in gliomas |
title | The role of transcriptional coactivator TAZ in gliomas |
title_full | The role of transcriptional coactivator TAZ in gliomas |
title_fullStr | The role of transcriptional coactivator TAZ in gliomas |
title_full_unstemmed | The role of transcriptional coactivator TAZ in gliomas |
title_short | The role of transcriptional coactivator TAZ in gliomas |
title_sort | role of transcriptional coactivator taz in gliomas |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5347724/ https://www.ncbi.nlm.nih.gov/pubmed/27764783 http://dx.doi.org/10.18632/oncotarget.12625 |
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