RNA interference: learning gene knock-down from cell physiology

Over the past decade RNA interference (RNAi) has emerged as a natural mechanism for silencing gene expression. This ancient cellular antiviral response can be exploited to allow specific inhibition of the function of any chosen target gene. RNAi is proving to be an invaluable research tool, allowing...

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Detalles Bibliográficos
Autores principales: Mocellin, Simone, Provenzano, Maurizio
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2004
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC534783/
https://www.ncbi.nlm.nih.gov/pubmed/15555080
http://dx.doi.org/10.1186/1479-5876-2-39
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author Mocellin, Simone
Provenzano, Maurizio
author_facet Mocellin, Simone
Provenzano, Maurizio
author_sort Mocellin, Simone
collection PubMed
description Over the past decade RNA interference (RNAi) has emerged as a natural mechanism for silencing gene expression. This ancient cellular antiviral response can be exploited to allow specific inhibition of the function of any chosen target gene. RNAi is proving to be an invaluable research tool, allowing much more rapid characterization of the function of known genes. More importantly, RNAi technology considerably bolsters functional genomics to aid in the identification of novel genes involved in disease processes. This review briefly describes the molecular principles underlying the biology of RNAi phenomenon and discuss the main technical issues regarding optimization of RNAi experimental design.
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spelling pubmed-5347832004-12-04 RNA interference: learning gene knock-down from cell physiology Mocellin, Simone Provenzano, Maurizio J Transl Med Review Over the past decade RNA interference (RNAi) has emerged as a natural mechanism for silencing gene expression. This ancient cellular antiviral response can be exploited to allow specific inhibition of the function of any chosen target gene. RNAi is proving to be an invaluable research tool, allowing much more rapid characterization of the function of known genes. More importantly, RNAi technology considerably bolsters functional genomics to aid in the identification of novel genes involved in disease processes. This review briefly describes the molecular principles underlying the biology of RNAi phenomenon and discuss the main technical issues regarding optimization of RNAi experimental design. BioMed Central 2004-11-22 /pmc/articles/PMC534783/ /pubmed/15555080 http://dx.doi.org/10.1186/1479-5876-2-39 Text en Copyright © 2004 Mocellin and Provenzano; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Review
Mocellin, Simone
Provenzano, Maurizio
RNA interference: learning gene knock-down from cell physiology
title RNA interference: learning gene knock-down from cell physiology
title_full RNA interference: learning gene knock-down from cell physiology
title_fullStr RNA interference: learning gene knock-down from cell physiology
title_full_unstemmed RNA interference: learning gene knock-down from cell physiology
title_short RNA interference: learning gene knock-down from cell physiology
title_sort rna interference: learning gene knock-down from cell physiology
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC534783/
https://www.ncbi.nlm.nih.gov/pubmed/15555080
http://dx.doi.org/10.1186/1479-5876-2-39
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