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Microarray profiling of circular RNAs in human papillary thyroid carcinoma

BACKGROUND: Non-coding circular RNAs (circRNAs) have displayed dysregulated expression in several human cancers. Here, we profiled the circRNA expression of papillary thyroid carcinoma (PTC) tumors to improve our understanding of PTC pathogenesis. METHODS: Microarray profiling was performed on 18 th...

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Autores principales: Peng, Nianchun, Shi, Lixin, Zhang, Qiao, Hu, Ying, Wang, Nanpeng, Ye, Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5347999/
https://www.ncbi.nlm.nih.gov/pubmed/28288173
http://dx.doi.org/10.1371/journal.pone.0170287
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author Peng, Nianchun
Shi, Lixin
Zhang, Qiao
Hu, Ying
Wang, Nanpeng
Ye, Hui
author_facet Peng, Nianchun
Shi, Lixin
Zhang, Qiao
Hu, Ying
Wang, Nanpeng
Ye, Hui
author_sort Peng, Nianchun
collection PubMed
description BACKGROUND: Non-coding circular RNAs (circRNAs) have displayed dysregulated expression in several human cancers. Here, we profiled the circRNA expression of papillary thyroid carcinoma (PTC) tumors to improve our understanding of PTC pathogenesis. METHODS: Microarray profiling was performed on 18 thyroid samples, consisting of six PTC tumors, six matching contralateral normal samples, and six benign thyroid lesions. After low-intensity filtering, hierarchical clustering revealed the circRNA expression patterns. Statistical analysis followed by qRT-PCR validation identified the differential circRNAs. MicroRNA (miRNA) target prediction software identified putative miRNA response elements (MREs), which were used to construct a network map of circRNA-miRNA interactions for the differential circRNAs. Bioinformatics platforms predicted cancer-related circRNA-miRNA associations and putative downstream target genes, respectively. RESULTS: A total of 88 circRNAs and 10 circRNAs were significantly upregulated and downregulated, respectively, in PTC tumors relative to normal thyroid tissue, while 129 circRNAs and 226 circRNAs were significantly upregulated and downregulated, respectively, in PTC tumors relative to benign thyroid lesions. A total of 12 upregulated and four downregulated circRNAs were overlapping between the foregoing comparisons. One downregulated circRNA (hsa_circRNA_100395) showed interactive potential with two cancer-related miRNAs (miR-141-3p and miR-200a-3p). From this analysis, we identified several promising cancer-related genes that may be targets of the dysregulated hsa_circRNA_100395/miR-141-3p/miR-200a-3p axis in PTC tumors. CONCLUSIONS: circRNA dysregulation may play a role in PTC pathogenesis, and several key circRNAs show promise as candidate biomarkers for PTC. The hsa_circRNA_100395/miR-141-3p/ miR-200a-3p axis may be involved in the pathogenesis of PTC.
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spelling pubmed-53479992017-03-30 Microarray profiling of circular RNAs in human papillary thyroid carcinoma Peng, Nianchun Shi, Lixin Zhang, Qiao Hu, Ying Wang, Nanpeng Ye, Hui PLoS One Research Article BACKGROUND: Non-coding circular RNAs (circRNAs) have displayed dysregulated expression in several human cancers. Here, we profiled the circRNA expression of papillary thyroid carcinoma (PTC) tumors to improve our understanding of PTC pathogenesis. METHODS: Microarray profiling was performed on 18 thyroid samples, consisting of six PTC tumors, six matching contralateral normal samples, and six benign thyroid lesions. After low-intensity filtering, hierarchical clustering revealed the circRNA expression patterns. Statistical analysis followed by qRT-PCR validation identified the differential circRNAs. MicroRNA (miRNA) target prediction software identified putative miRNA response elements (MREs), which were used to construct a network map of circRNA-miRNA interactions for the differential circRNAs. Bioinformatics platforms predicted cancer-related circRNA-miRNA associations and putative downstream target genes, respectively. RESULTS: A total of 88 circRNAs and 10 circRNAs were significantly upregulated and downregulated, respectively, in PTC tumors relative to normal thyroid tissue, while 129 circRNAs and 226 circRNAs were significantly upregulated and downregulated, respectively, in PTC tumors relative to benign thyroid lesions. A total of 12 upregulated and four downregulated circRNAs were overlapping between the foregoing comparisons. One downregulated circRNA (hsa_circRNA_100395) showed interactive potential with two cancer-related miRNAs (miR-141-3p and miR-200a-3p). From this analysis, we identified several promising cancer-related genes that may be targets of the dysregulated hsa_circRNA_100395/miR-141-3p/miR-200a-3p axis in PTC tumors. CONCLUSIONS: circRNA dysregulation may play a role in PTC pathogenesis, and several key circRNAs show promise as candidate biomarkers for PTC. The hsa_circRNA_100395/miR-141-3p/ miR-200a-3p axis may be involved in the pathogenesis of PTC. Public Library of Science 2017-03-13 /pmc/articles/PMC5347999/ /pubmed/28288173 http://dx.doi.org/10.1371/journal.pone.0170287 Text en © 2017 Peng et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Peng, Nianchun
Shi, Lixin
Zhang, Qiao
Hu, Ying
Wang, Nanpeng
Ye, Hui
Microarray profiling of circular RNAs in human papillary thyroid carcinoma
title Microarray profiling of circular RNAs in human papillary thyroid carcinoma
title_full Microarray profiling of circular RNAs in human papillary thyroid carcinoma
title_fullStr Microarray profiling of circular RNAs in human papillary thyroid carcinoma
title_full_unstemmed Microarray profiling of circular RNAs in human papillary thyroid carcinoma
title_short Microarray profiling of circular RNAs in human papillary thyroid carcinoma
title_sort microarray profiling of circular rnas in human papillary thyroid carcinoma
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5347999/
https://www.ncbi.nlm.nih.gov/pubmed/28288173
http://dx.doi.org/10.1371/journal.pone.0170287
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