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A viral strategy for targeting and manipulating interneurons across vertebrate species
A fundamental impediment to understanding the brain is the availability of inexpensive and robust methods for targeting and manipulating specific neuronal populations. The need to overcome this barrier is pressing because there are considerable anatomical, physiological, cognitive, and behavioral di...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5348112/ https://www.ncbi.nlm.nih.gov/pubmed/27798629 http://dx.doi.org/10.1038/nn.4430 |
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author | Dimidschstein, J Chen, Q Tremblay, R Rogers, SL Saldi, GA Guo, L Xu, C Liu, R Lu, C Chu, J Avery, MC Rashid, SM Baek, M Jacob, AL Smith, GB Wilson, DE Kosche, G Kruglikov, I Rusielewicz, T Kotak, VC Mowery, TM Anderson, SA Callaway, EM Dasen, JS Fitzpatrick, D Fossati, V Long, MA Noggle, S Reynolds, JH Sanes, DH Rudy, B Feng, G Fishell, G |
author_facet | Dimidschstein, J Chen, Q Tremblay, R Rogers, SL Saldi, GA Guo, L Xu, C Liu, R Lu, C Chu, J Avery, MC Rashid, SM Baek, M Jacob, AL Smith, GB Wilson, DE Kosche, G Kruglikov, I Rusielewicz, T Kotak, VC Mowery, TM Anderson, SA Callaway, EM Dasen, JS Fitzpatrick, D Fossati, V Long, MA Noggle, S Reynolds, JH Sanes, DH Rudy, B Feng, G Fishell, G |
author_sort | Dimidschstein, J |
collection | PubMed |
description | A fundamental impediment to understanding the brain is the availability of inexpensive and robust methods for targeting and manipulating specific neuronal populations. The need to overcome this barrier is pressing because there are considerable anatomical, physiological, cognitive, and behavioral differences between mice and higher mammalian species in which it is difficult to specifically target and manipulate genetically defined functional cell-types. In particular, it is unclear the degree to which insights from mouse models can shed light on the neural mechanisms that mediate cognitive functions in higher species including humans. Here we describe a novel recombinant adeno-associated virus (rAAV) that restricts gene expression to GABAergic interneurons within the telencephalon. We demonstrate that the viral expression is specific and robust, allowing for morphological visualization, activity monitoring and functional manipulation of interneurons in both mice and non-genetically tractable species, thus opening the possibility to study GABA-ergic function in virtually any vertebrate species. |
format | Online Article Text |
id | pubmed-5348112 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
record_format | MEDLINE/PubMed |
spelling | pubmed-53481122017-04-30 A viral strategy for targeting and manipulating interneurons across vertebrate species Dimidschstein, J Chen, Q Tremblay, R Rogers, SL Saldi, GA Guo, L Xu, C Liu, R Lu, C Chu, J Avery, MC Rashid, SM Baek, M Jacob, AL Smith, GB Wilson, DE Kosche, G Kruglikov, I Rusielewicz, T Kotak, VC Mowery, TM Anderson, SA Callaway, EM Dasen, JS Fitzpatrick, D Fossati, V Long, MA Noggle, S Reynolds, JH Sanes, DH Rudy, B Feng, G Fishell, G Nat Neurosci Article A fundamental impediment to understanding the brain is the availability of inexpensive and robust methods for targeting and manipulating specific neuronal populations. The need to overcome this barrier is pressing because there are considerable anatomical, physiological, cognitive, and behavioral differences between mice and higher mammalian species in which it is difficult to specifically target and manipulate genetically defined functional cell-types. In particular, it is unclear the degree to which insights from mouse models can shed light on the neural mechanisms that mediate cognitive functions in higher species including humans. Here we describe a novel recombinant adeno-associated virus (rAAV) that restricts gene expression to GABAergic interneurons within the telencephalon. We demonstrate that the viral expression is specific and robust, allowing for morphological visualization, activity monitoring and functional manipulation of interneurons in both mice and non-genetically tractable species, thus opening the possibility to study GABA-ergic function in virtually any vertebrate species. 2016-10-31 2016-12 /pmc/articles/PMC5348112/ /pubmed/27798629 http://dx.doi.org/10.1038/nn.4430 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Dimidschstein, J Chen, Q Tremblay, R Rogers, SL Saldi, GA Guo, L Xu, C Liu, R Lu, C Chu, J Avery, MC Rashid, SM Baek, M Jacob, AL Smith, GB Wilson, DE Kosche, G Kruglikov, I Rusielewicz, T Kotak, VC Mowery, TM Anderson, SA Callaway, EM Dasen, JS Fitzpatrick, D Fossati, V Long, MA Noggle, S Reynolds, JH Sanes, DH Rudy, B Feng, G Fishell, G A viral strategy for targeting and manipulating interneurons across vertebrate species |
title | A viral strategy for targeting and manipulating interneurons across vertebrate species |
title_full | A viral strategy for targeting and manipulating interneurons across vertebrate species |
title_fullStr | A viral strategy for targeting and manipulating interneurons across vertebrate species |
title_full_unstemmed | A viral strategy for targeting and manipulating interneurons across vertebrate species |
title_short | A viral strategy for targeting and manipulating interneurons across vertebrate species |
title_sort | viral strategy for targeting and manipulating interneurons across vertebrate species |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5348112/ https://www.ncbi.nlm.nih.gov/pubmed/27798629 http://dx.doi.org/10.1038/nn.4430 |
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