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Co-expression of Lgr5 and CXCR4 characterizes cancer stem-like cells of colorectal cancer
Therapies designed to target cancer stem cells (CSCs) in colorectal cancer (CRC) may improve treatment outcomes. Different markers have been used to identify CSCs or CSC-like cells in CRC, but the enrichment of CSCs using these markers has yet to be optimized. We recently reported the importance of...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5348382/ https://www.ncbi.nlm.nih.gov/pubmed/27835894 http://dx.doi.org/10.18632/oncotarget.13214 |
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author | Wu, Weidong Cao, Jun Ji, Zhengyi Wang, Jingjue Jiang, Tao Ding, Honghua |
author_facet | Wu, Weidong Cao, Jun Ji, Zhengyi Wang, Jingjue Jiang, Tao Ding, Honghua |
author_sort | Wu, Weidong |
collection | PubMed |
description | Therapies designed to target cancer stem cells (CSCs) in colorectal cancer (CRC) may improve treatment outcomes. Different markers have been used to identify CSCs or CSC-like cells in CRC, but the enrichment of CSCs using these markers has yet to be optimized. We recently reported the importance of Lgr5-positive CRC cells in cancer growth. Here, we studied the possibility of using Lgr5 and CXCR4 as CSC markers for CRC. We detected high Lgr5 and CXCR4 levels in stage IV CRC specimens. Both high Lgr5 and CXCR4 levels were associated with poor prognosis in stage IV CRC patients. In vitro, Lgr5+CXCR4-, CXCR4+Lgr5- and Lgr5+CXCR4+ cells were purified in human CRC cell lines and examined for their CSC properties. We found that compared to the unsorted cells, CXCR4+Lgr5-, Lgr5+CXCR4-, and Lgr5+/CXCR4+ cells showed significantly greater cancer mass after subcutaneous transplantation, greater tumor sphere formation, higher resistance to chemotherapy, and higher incidence of tumor formation after serial adoptive transplantation into NOD/SCID mice. Taken together, our data suggest that the combined use of Lgr5 and CXCR4 may facilitate the enrichment of CSCs in CRC, and that treating Lgr5+/CXCR4+ CRC cells may improve the outcome of CRC therapy. |
format | Online Article Text |
id | pubmed-5348382 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-53483822017-03-31 Co-expression of Lgr5 and CXCR4 characterizes cancer stem-like cells of colorectal cancer Wu, Weidong Cao, Jun Ji, Zhengyi Wang, Jingjue Jiang, Tao Ding, Honghua Oncotarget Research Paper Therapies designed to target cancer stem cells (CSCs) in colorectal cancer (CRC) may improve treatment outcomes. Different markers have been used to identify CSCs or CSC-like cells in CRC, but the enrichment of CSCs using these markers has yet to be optimized. We recently reported the importance of Lgr5-positive CRC cells in cancer growth. Here, we studied the possibility of using Lgr5 and CXCR4 as CSC markers for CRC. We detected high Lgr5 and CXCR4 levels in stage IV CRC specimens. Both high Lgr5 and CXCR4 levels were associated with poor prognosis in stage IV CRC patients. In vitro, Lgr5+CXCR4-, CXCR4+Lgr5- and Lgr5+CXCR4+ cells were purified in human CRC cell lines and examined for their CSC properties. We found that compared to the unsorted cells, CXCR4+Lgr5-, Lgr5+CXCR4-, and Lgr5+/CXCR4+ cells showed significantly greater cancer mass after subcutaneous transplantation, greater tumor sphere formation, higher resistance to chemotherapy, and higher incidence of tumor formation after serial adoptive transplantation into NOD/SCID mice. Taken together, our data suggest that the combined use of Lgr5 and CXCR4 may facilitate the enrichment of CSCs in CRC, and that treating Lgr5+/CXCR4+ CRC cells may improve the outcome of CRC therapy. Impact Journals LLC 2016-11-08 /pmc/articles/PMC5348382/ /pubmed/27835894 http://dx.doi.org/10.18632/oncotarget.13214 Text en Copyright: © 2016 Wu et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Wu, Weidong Cao, Jun Ji, Zhengyi Wang, Jingjue Jiang, Tao Ding, Honghua Co-expression of Lgr5 and CXCR4 characterizes cancer stem-like cells of colorectal cancer |
title | Co-expression of Lgr5 and CXCR4 characterizes cancer stem-like cells of colorectal cancer |
title_full | Co-expression of Lgr5 and CXCR4 characterizes cancer stem-like cells of colorectal cancer |
title_fullStr | Co-expression of Lgr5 and CXCR4 characterizes cancer stem-like cells of colorectal cancer |
title_full_unstemmed | Co-expression of Lgr5 and CXCR4 characterizes cancer stem-like cells of colorectal cancer |
title_short | Co-expression of Lgr5 and CXCR4 characterizes cancer stem-like cells of colorectal cancer |
title_sort | co-expression of lgr5 and cxcr4 characterizes cancer stem-like cells of colorectal cancer |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5348382/ https://www.ncbi.nlm.nih.gov/pubmed/27835894 http://dx.doi.org/10.18632/oncotarget.13214 |
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