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Long noncoding RNA BX357664 regulates cell proliferation and epithelial-to-mesenchymal transition via inhibition of TGF-β1/p38/HSP27 signaling in renal cell carcinoma
Antisense long noncoding RNAs (lncRNAs) are reported to play a regulating role in carcinogenesis of various human malignancies. However, the function of lncRNAs and their underlying mechanism in renal cell carcinoma (RCC) is still unknown. The aims of this study are to investigate the expression of...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5348402/ https://www.ncbi.nlm.nih.gov/pubmed/27806310 http://dx.doi.org/10.18632/oncotarget.12937 |
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author | Liu, Yiyang Qian, Jian Li, Xiao Chen, Wei Xu, Aiming Zhao, Kai Hua, Yibo Huang, Zhengkai Zhang, Jianzhong Liang, Chao Su, Shifeng Li, Pu Shao, Pengfei Li, Jie Qin, Chao Wang, Zengjun |
author_facet | Liu, Yiyang Qian, Jian Li, Xiao Chen, Wei Xu, Aiming Zhao, Kai Hua, Yibo Huang, Zhengkai Zhang, Jianzhong Liang, Chao Su, Shifeng Li, Pu Shao, Pengfei Li, Jie Qin, Chao Wang, Zengjun |
author_sort | Liu, Yiyang |
collection | PubMed |
description | Antisense long noncoding RNAs (lncRNAs) are reported to play a regulating role in carcinogenesis of various human malignancies. However, the function of lncRNAs and their underlying mechanism in renal cell carcinoma (RCC) is still unknown. The aims of this study are to investigate the expression of lncRNA BX357664 in RCC and to explore its function in RCC cell lines. As a result, BX357664 was downregulated in RCC according to previous microarray analysis and qualitative real-time polymerase chain reaction. After the upregulation of BX357664, reduced migration, invasion, and proliferation capabilities in RCC cells were detected. Furthermore, Western blot analysis was conducted to identify the influence of BX357664 on epithelial-to-mesenchymal transition, matrix metalloproteinase 2, matrix metalloproteinase 9, and transforming growth factor-beta 1 (TGF-β1)/p38/HSP27 signaling pathway in RCC. Subsequently, upregulating the protein level of TGF-β1 in the presence of BX357664 could rescue the suppression of the malignant behavior mediated by BX357664, indicating that BX357664 attributed its inhibitory role to the suppression of TGF-β1. Therefore, we investigated a novel lncRNA BX357664, which might exhibit its inhibitory role in RCC metastasis and progression by blocking the TGF-β1/p38/HSP27 pathway. |
format | Online Article Text |
id | pubmed-5348402 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-53484022017-03-31 Long noncoding RNA BX357664 regulates cell proliferation and epithelial-to-mesenchymal transition via inhibition of TGF-β1/p38/HSP27 signaling in renal cell carcinoma Liu, Yiyang Qian, Jian Li, Xiao Chen, Wei Xu, Aiming Zhao, Kai Hua, Yibo Huang, Zhengkai Zhang, Jianzhong Liang, Chao Su, Shifeng Li, Pu Shao, Pengfei Li, Jie Qin, Chao Wang, Zengjun Oncotarget Research Paper Antisense long noncoding RNAs (lncRNAs) are reported to play a regulating role in carcinogenesis of various human malignancies. However, the function of lncRNAs and their underlying mechanism in renal cell carcinoma (RCC) is still unknown. The aims of this study are to investigate the expression of lncRNA BX357664 in RCC and to explore its function in RCC cell lines. As a result, BX357664 was downregulated in RCC according to previous microarray analysis and qualitative real-time polymerase chain reaction. After the upregulation of BX357664, reduced migration, invasion, and proliferation capabilities in RCC cells were detected. Furthermore, Western blot analysis was conducted to identify the influence of BX357664 on epithelial-to-mesenchymal transition, matrix metalloproteinase 2, matrix metalloproteinase 9, and transforming growth factor-beta 1 (TGF-β1)/p38/HSP27 signaling pathway in RCC. Subsequently, upregulating the protein level of TGF-β1 in the presence of BX357664 could rescue the suppression of the malignant behavior mediated by BX357664, indicating that BX357664 attributed its inhibitory role to the suppression of TGF-β1. Therefore, we investigated a novel lncRNA BX357664, which might exhibit its inhibitory role in RCC metastasis and progression by blocking the TGF-β1/p38/HSP27 pathway. Impact Journals LLC 2016-10-27 /pmc/articles/PMC5348402/ /pubmed/27806310 http://dx.doi.org/10.18632/oncotarget.12937 Text en Copyright: © 2016 Liu et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Liu, Yiyang Qian, Jian Li, Xiao Chen, Wei Xu, Aiming Zhao, Kai Hua, Yibo Huang, Zhengkai Zhang, Jianzhong Liang, Chao Su, Shifeng Li, Pu Shao, Pengfei Li, Jie Qin, Chao Wang, Zengjun Long noncoding RNA BX357664 regulates cell proliferation and epithelial-to-mesenchymal transition via inhibition of TGF-β1/p38/HSP27 signaling in renal cell carcinoma |
title | Long noncoding RNA BX357664 regulates cell proliferation and epithelial-to-mesenchymal transition via inhibition of TGF-β1/p38/HSP27 signaling in renal cell carcinoma |
title_full | Long noncoding RNA BX357664 regulates cell proliferation and epithelial-to-mesenchymal transition via inhibition of TGF-β1/p38/HSP27 signaling in renal cell carcinoma |
title_fullStr | Long noncoding RNA BX357664 regulates cell proliferation and epithelial-to-mesenchymal transition via inhibition of TGF-β1/p38/HSP27 signaling in renal cell carcinoma |
title_full_unstemmed | Long noncoding RNA BX357664 regulates cell proliferation and epithelial-to-mesenchymal transition via inhibition of TGF-β1/p38/HSP27 signaling in renal cell carcinoma |
title_short | Long noncoding RNA BX357664 regulates cell proliferation and epithelial-to-mesenchymal transition via inhibition of TGF-β1/p38/HSP27 signaling in renal cell carcinoma |
title_sort | long noncoding rna bx357664 regulates cell proliferation and epithelial-to-mesenchymal transition via inhibition of tgf-β1/p38/hsp27 signaling in renal cell carcinoma |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5348402/ https://www.ncbi.nlm.nih.gov/pubmed/27806310 http://dx.doi.org/10.18632/oncotarget.12937 |
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