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Development of a Double Nuclear Gene-Targeting Method by Two-Step Transformation Based on a Newly Established Chloramphenicol-Selection System in the Red Alga Cyanidioschyzon merolae
The unicellular red alga Cyanidioschyzon merolae possesses a simple cellular architecture that consists of one mitochondrion, one chloroplast, one peroxisome, one Golgi apparatus, and several lysosomes. The nuclear genome content is also simple, with very little genetic redundancy (16.5 Mbp, 4,775 g...
Autores principales: | Fujiwara, Takayuki, Ohnuma, Mio, Kuroiwa, Tsuneyoshi, Ohbayashi, Ryudo, Hirooka, Shunsuke, Miyagishima, Shin-Ya |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5348525/ https://www.ncbi.nlm.nih.gov/pubmed/28352279 http://dx.doi.org/10.3389/fpls.2017.00343 |
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