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Guide Picker is a comprehensive design tool for visualizing and selecting guides for CRISPR experiments
BACKGROUND: Guide Picker (https://www.deskgen.com/guide-picker/) serves as a meta tool for designing CRISPR experiments by presenting ten different guide RNA scoring functions in one simple graphical interface. It allows investigators to simultaneously visualize and sort through every guide targetin...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5348774/ https://www.ncbi.nlm.nih.gov/pubmed/28288556 http://dx.doi.org/10.1186/s12859-017-1581-4 |
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author | Hough, Soren H. Kancleris, Kris Brody, Leigh Humphryes-Kirilov, Neil Wolanski, Joseph Dunaway, Keith Ajetunmobi, Ayokunmi Dillard, Victor |
author_facet | Hough, Soren H. Kancleris, Kris Brody, Leigh Humphryes-Kirilov, Neil Wolanski, Joseph Dunaway, Keith Ajetunmobi, Ayokunmi Dillard, Victor |
author_sort | Hough, Soren H. |
collection | PubMed |
description | BACKGROUND: Guide Picker (https://www.deskgen.com/guide-picker/) serves as a meta tool for designing CRISPR experiments by presenting ten different guide RNA scoring functions in one simple graphical interface. It allows investigators to simultaneously visualize and sort through every guide targeting the protein-coding regions of any mouse or human gene. RESULTS: Utilizing a multidimensional graphical display featuring two plots and four axes, Guide Picker can analyze all guides while filtering based on four different criteria at a time. Guide Picker further facilitates the CRISPR design process by using pre-computed scores for all guides, thereby offering rapid guide RNA generation and selection. CONCLUSIONS: The ease-of-use of Guide Picker complements CRISPR itself, matching a powerful and modular biological system with a flexible online web tool that can be used in a variety of genome editing experimental contexts. |
format | Online Article Text |
id | pubmed-5348774 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-53487742017-03-14 Guide Picker is a comprehensive design tool for visualizing and selecting guides for CRISPR experiments Hough, Soren H. Kancleris, Kris Brody, Leigh Humphryes-Kirilov, Neil Wolanski, Joseph Dunaway, Keith Ajetunmobi, Ayokunmi Dillard, Victor BMC Bioinformatics Software BACKGROUND: Guide Picker (https://www.deskgen.com/guide-picker/) serves as a meta tool for designing CRISPR experiments by presenting ten different guide RNA scoring functions in one simple graphical interface. It allows investigators to simultaneously visualize and sort through every guide targeting the protein-coding regions of any mouse or human gene. RESULTS: Utilizing a multidimensional graphical display featuring two plots and four axes, Guide Picker can analyze all guides while filtering based on four different criteria at a time. Guide Picker further facilitates the CRISPR design process by using pre-computed scores for all guides, thereby offering rapid guide RNA generation and selection. CONCLUSIONS: The ease-of-use of Guide Picker complements CRISPR itself, matching a powerful and modular biological system with a flexible online web tool that can be used in a variety of genome editing experimental contexts. BioMed Central 2017-03-14 /pmc/articles/PMC5348774/ /pubmed/28288556 http://dx.doi.org/10.1186/s12859-017-1581-4 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Software Hough, Soren H. Kancleris, Kris Brody, Leigh Humphryes-Kirilov, Neil Wolanski, Joseph Dunaway, Keith Ajetunmobi, Ayokunmi Dillard, Victor Guide Picker is a comprehensive design tool for visualizing and selecting guides for CRISPR experiments |
title | Guide Picker is a comprehensive design tool for visualizing and selecting guides for CRISPR experiments |
title_full | Guide Picker is a comprehensive design tool for visualizing and selecting guides for CRISPR experiments |
title_fullStr | Guide Picker is a comprehensive design tool for visualizing and selecting guides for CRISPR experiments |
title_full_unstemmed | Guide Picker is a comprehensive design tool for visualizing and selecting guides for CRISPR experiments |
title_short | Guide Picker is a comprehensive design tool for visualizing and selecting guides for CRISPR experiments |
title_sort | guide picker is a comprehensive design tool for visualizing and selecting guides for crispr experiments |
topic | Software |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5348774/ https://www.ncbi.nlm.nih.gov/pubmed/28288556 http://dx.doi.org/10.1186/s12859-017-1581-4 |
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