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Nanotechnology in ligature-induced periodontitis: protective effect of a doxycycline gel with nanoparticules

OBJECTIVES: The aim of this study was to test the efficacy of a locally applied 8.5% nanostructured doxycycline (DOX) gel in preventing alveolar bone loss in experimental periodontal disease (ePD) in rats by using the tapping mode atomic force microscopy (AFM). MATERIAL AND METHODS: ePD was induced...

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Autores principales: BOTELHO, Marco Antonio, MARTINS, Jose Galberto, RUELA, Ronaldo Sousa, QUEIROZ, Dinalva Brito, RUELA, Wagner Sousa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Faculdade de Odontologia de Bauru da Universidade de São Paulo 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5349078/
https://www.ncbi.nlm.nih.gov/pubmed/20835566
http://dx.doi.org/10.1590/S1678-77572010000400003
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author BOTELHO, Marco Antonio
MARTINS, Jose Galberto
RUELA, Ronaldo Sousa
QUEIROZ, Dinalva Brito
RUELA, Wagner Sousa
author_facet BOTELHO, Marco Antonio
MARTINS, Jose Galberto
RUELA, Ronaldo Sousa
QUEIROZ, Dinalva Brito
RUELA, Wagner Sousa
author_sort BOTELHO, Marco Antonio
collection PubMed
description OBJECTIVES: The aim of this study was to test the efficacy of a locally applied 8.5% nanostructured doxycycline (DOX) gel in preventing alveolar bone loss in experimental periodontal disease (ePD) in rats by using the tapping mode atomic force microscopy (AFM). MATERIAL AND METHODS: ePD was induced in 24 Wistar rats. Animals were treated with the doxycycline gel topically, immediately after ePD induction, and 3 times a day during 11 days. Four groups (n=6) were formed as follows: Naïve group (animals not subjected to ePD nor treated); non-treated (NT) group (animals subjected to ePD, but not treated); vehicle gel (VG) group (animals subjected to ePD and treated with topical gel vehicle); and DOX group (test group): animals subjected to ePD and treated with the 8.5% DOX gel. In order to investigate topographical changes in histological sections, a novel simple method was used for sample preparation, by etching sections from paraffin-embedded specimens with xylol. RESULTS: Comparing the AFM images, several grooves were observed on the surface of the alveolar bone and other periodontal structures in the NT and VG groups, with significantly greater depths when compared to the DOX group (p<0.05). CONCLUSIONS: Periodontal structures were brought into high relief confirming to be a simple and costeffective method for AFM imaging with ultrastructural resolution. The doxycycline gel was able to afford periodontal surface preservation, with flatter grooves.
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spelling pubmed-53490782017-03-17 Nanotechnology in ligature-induced periodontitis: protective effect of a doxycycline gel with nanoparticules BOTELHO, Marco Antonio MARTINS, Jose Galberto RUELA, Ronaldo Sousa QUEIROZ, Dinalva Brito RUELA, Wagner Sousa J Appl Oral Sci Original Articles OBJECTIVES: The aim of this study was to test the efficacy of a locally applied 8.5% nanostructured doxycycline (DOX) gel in preventing alveolar bone loss in experimental periodontal disease (ePD) in rats by using the tapping mode atomic force microscopy (AFM). MATERIAL AND METHODS: ePD was induced in 24 Wistar rats. Animals were treated with the doxycycline gel topically, immediately after ePD induction, and 3 times a day during 11 days. Four groups (n=6) were formed as follows: Naïve group (animals not subjected to ePD nor treated); non-treated (NT) group (animals subjected to ePD, but not treated); vehicle gel (VG) group (animals subjected to ePD and treated with topical gel vehicle); and DOX group (test group): animals subjected to ePD and treated with the 8.5% DOX gel. In order to investigate topographical changes in histological sections, a novel simple method was used for sample preparation, by etching sections from paraffin-embedded specimens with xylol. RESULTS: Comparing the AFM images, several grooves were observed on the surface of the alveolar bone and other periodontal structures in the NT and VG groups, with significantly greater depths when compared to the DOX group (p<0.05). CONCLUSIONS: Periodontal structures were brought into high relief confirming to be a simple and costeffective method for AFM imaging with ultrastructural resolution. The doxycycline gel was able to afford periodontal surface preservation, with flatter grooves. Faculdade de Odontologia de Bauru da Universidade de São Paulo 2010 /pmc/articles/PMC5349078/ /pubmed/20835566 http://dx.doi.org/10.1590/S1678-77572010000400003 Text en http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
BOTELHO, Marco Antonio
MARTINS, Jose Galberto
RUELA, Ronaldo Sousa
QUEIROZ, Dinalva Brito
RUELA, Wagner Sousa
Nanotechnology in ligature-induced periodontitis: protective effect of a doxycycline gel with nanoparticules
title Nanotechnology in ligature-induced periodontitis: protective effect of a doxycycline gel with nanoparticules
title_full Nanotechnology in ligature-induced periodontitis: protective effect of a doxycycline gel with nanoparticules
title_fullStr Nanotechnology in ligature-induced periodontitis: protective effect of a doxycycline gel with nanoparticules
title_full_unstemmed Nanotechnology in ligature-induced periodontitis: protective effect of a doxycycline gel with nanoparticules
title_short Nanotechnology in ligature-induced periodontitis: protective effect of a doxycycline gel with nanoparticules
title_sort nanotechnology in ligature-induced periodontitis: protective effect of a doxycycline gel with nanoparticules
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5349078/
https://www.ncbi.nlm.nih.gov/pubmed/20835566
http://dx.doi.org/10.1590/S1678-77572010000400003
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