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Effect of paraquat-induced oxidative stress on gene expression and aging of the filamentous ascomycete Podospora anserina
Aging of biological systems is influenced by various factors, conditions and processes. Among others, processes allowing organisms to deal with various types of stress are of key importance. In particular, oxidative stress as the result of the generation of reactive oxygen species (ROS) at the mitoc...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Shared Science Publishers OG
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5349155/ https://www.ncbi.nlm.nih.gov/pubmed/28357247 http://dx.doi.org/10.15698/mic2014.07.155 |
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author | Wiemer, Matthias Osiewacz, Heinz D. |
author_facet | Wiemer, Matthias Osiewacz, Heinz D. |
author_sort | Wiemer, Matthias |
collection | PubMed |
description | Aging of biological systems is influenced by various factors, conditions and processes. Among others, processes allowing organisms to deal with various types of stress are of key importance. In particular, oxidative stress as the result of the generation of reactive oxygen species (ROS) at the mitochondrial respiratory chain and the accumulation of ROS-induced molecular damage has been strongly linked to aging. Here we view the impact of ROS from a different angle: their role in the control of gene expression. We report a genome-wide transcriptome analysis of the fungal aging model Podospora anserina grown on medium containing paraquat (PQ). This treatment leads to an increased cellular generation and release of H(2)O(2), a reduced growth rate, and a decrease in lifespan. The combined challenge by PQ and copper has a synergistic negative effect on growth and lifespan. The data from the transcriptome analysis of the wild type cultivated under PQ-stress and their comparison to those of a longitudinal aging study as well as of a copper-uptake longevity mutant of P. anserina revealed that PQ-stress leads to the up-regulation of transcripts coding for components involved in mitochondrial remodeling. PQ also affects the expression of copper-regulated genes suggesting an increase of cytoplasmic copper levels as it has been demonstrated earlier to occur during aging of P. anserina and during senescence of human fibroblasts. This effect may result from the induction of the mitochondrial permeability transition pore via PQ-induced ROS, leading to programmed cell death as part of an evolutionary conserved mechanism involved in biological aging and lifespan control. |
format | Online Article Text |
id | pubmed-5349155 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Shared Science Publishers OG |
record_format | MEDLINE/PubMed |
spelling | pubmed-53491552017-03-29 Effect of paraquat-induced oxidative stress on gene expression and aging of the filamentous ascomycete Podospora anserina Wiemer, Matthias Osiewacz, Heinz D. Microb Cell Microbiology Aging of biological systems is influenced by various factors, conditions and processes. Among others, processes allowing organisms to deal with various types of stress are of key importance. In particular, oxidative stress as the result of the generation of reactive oxygen species (ROS) at the mitochondrial respiratory chain and the accumulation of ROS-induced molecular damage has been strongly linked to aging. Here we view the impact of ROS from a different angle: their role in the control of gene expression. We report a genome-wide transcriptome analysis of the fungal aging model Podospora anserina grown on medium containing paraquat (PQ). This treatment leads to an increased cellular generation and release of H(2)O(2), a reduced growth rate, and a decrease in lifespan. The combined challenge by PQ and copper has a synergistic negative effect on growth and lifespan. The data from the transcriptome analysis of the wild type cultivated under PQ-stress and their comparison to those of a longitudinal aging study as well as of a copper-uptake longevity mutant of P. anserina revealed that PQ-stress leads to the up-regulation of transcripts coding for components involved in mitochondrial remodeling. PQ also affects the expression of copper-regulated genes suggesting an increase of cytoplasmic copper levels as it has been demonstrated earlier to occur during aging of P. anserina and during senescence of human fibroblasts. This effect may result from the induction of the mitochondrial permeability transition pore via PQ-induced ROS, leading to programmed cell death as part of an evolutionary conserved mechanism involved in biological aging and lifespan control. Shared Science Publishers OG 2014-06-22 /pmc/articles/PMC5349155/ /pubmed/28357247 http://dx.doi.org/10.15698/mic2014.07.155 Text en https://creativecommons.org/licenses/by/4.0/ This is an open-access article released under the terms of the Creative Commons Attribution (CC BY) license, which allows the unrestricted use, distribution, and reproduction in any medium, provided the original author and source are acknowledged. |
spellingShingle | Microbiology Wiemer, Matthias Osiewacz, Heinz D. Effect of paraquat-induced oxidative stress on gene expression and aging of the filamentous ascomycete Podospora anserina |
title | Effect of paraquat-induced oxidative stress on gene expression and aging of the filamentous
ascomycete Podospora anserina |
title_full | Effect of paraquat-induced oxidative stress on gene expression and aging of the filamentous
ascomycete Podospora anserina |
title_fullStr | Effect of paraquat-induced oxidative stress on gene expression and aging of the filamentous
ascomycete Podospora anserina |
title_full_unstemmed | Effect of paraquat-induced oxidative stress on gene expression and aging of the filamentous
ascomycete Podospora anserina |
title_short | Effect of paraquat-induced oxidative stress on gene expression and aging of the filamentous
ascomycete Podospora anserina |
title_sort | effect of paraquat-induced oxidative stress on gene expression and aging of the filamentous
ascomycete podospora anserina |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5349155/ https://www.ncbi.nlm.nih.gov/pubmed/28357247 http://dx.doi.org/10.15698/mic2014.07.155 |
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