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The core protein of a pestivirus protects the incoming virus against IFN-induced effectors

A multitude of viral factors - either inhibiting the induction of the IFN-system or its effectors – have been described to date. However, little is known about the role of structural components of the incoming virus particle in protecting against IFN-induced antiviral factors during or immediately a...

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Autores principales: Riedel, Christiane, Lamp, Benjamin, Hagen, Benedikt, Indik, Stanislav, Rümenapf, Till
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5349576/
https://www.ncbi.nlm.nih.gov/pubmed/28290554
http://dx.doi.org/10.1038/srep44459
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author Riedel, Christiane
Lamp, Benjamin
Hagen, Benedikt
Indik, Stanislav
Rümenapf, Till
author_facet Riedel, Christiane
Lamp, Benjamin
Hagen, Benedikt
Indik, Stanislav
Rümenapf, Till
author_sort Riedel, Christiane
collection PubMed
description A multitude of viral factors - either inhibiting the induction of the IFN-system or its effectors – have been described to date. However, little is known about the role of structural components of the incoming virus particle in protecting against IFN-induced antiviral factors during or immediately after entry. In this study, we take advantage of the previously reported property of Classical swine fever virus (family Flaviviridae, genus Pestivirus) to tolerate a deletion of the core protein if a compensatory mutation is present in the NS3-helicase-domain (Vp447(∆c)). In contrast to the parental virus (Vp447), which causes a hemorrhagic-fever-like disease in pigs, Vp447(∆c) is avirulent in vivo. In comparison to Vp447, growth of Vp447(∆c) in primary porcine cells and IFN-treated porcine cell lines was reduced >20-fold. Also, primary porcine endothelial cells and IFN-pretreated porcine cell lines were 8–24 times less susceptible to Vp447(∆c). This reduction of susceptibility could be partially reversed by loading Vp447(∆c) particles with different levels of core protein. In contrast, expression of core protein in the recipient cell did not have any beneficial effect. Therefore, a protective effect of core protein in the incoming virus particle against the products of IFN-stimulated genes could be demonstrated.
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spelling pubmed-53495762017-03-17 The core protein of a pestivirus protects the incoming virus against IFN-induced effectors Riedel, Christiane Lamp, Benjamin Hagen, Benedikt Indik, Stanislav Rümenapf, Till Sci Rep Article A multitude of viral factors - either inhibiting the induction of the IFN-system or its effectors – have been described to date. However, little is known about the role of structural components of the incoming virus particle in protecting against IFN-induced antiviral factors during or immediately after entry. In this study, we take advantage of the previously reported property of Classical swine fever virus (family Flaviviridae, genus Pestivirus) to tolerate a deletion of the core protein if a compensatory mutation is present in the NS3-helicase-domain (Vp447(∆c)). In contrast to the parental virus (Vp447), which causes a hemorrhagic-fever-like disease in pigs, Vp447(∆c) is avirulent in vivo. In comparison to Vp447, growth of Vp447(∆c) in primary porcine cells and IFN-treated porcine cell lines was reduced >20-fold. Also, primary porcine endothelial cells and IFN-pretreated porcine cell lines were 8–24 times less susceptible to Vp447(∆c). This reduction of susceptibility could be partially reversed by loading Vp447(∆c) particles with different levels of core protein. In contrast, expression of core protein in the recipient cell did not have any beneficial effect. Therefore, a protective effect of core protein in the incoming virus particle against the products of IFN-stimulated genes could be demonstrated. Nature Publishing Group 2017-03-14 /pmc/articles/PMC5349576/ /pubmed/28290554 http://dx.doi.org/10.1038/srep44459 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Riedel, Christiane
Lamp, Benjamin
Hagen, Benedikt
Indik, Stanislav
Rümenapf, Till
The core protein of a pestivirus protects the incoming virus against IFN-induced effectors
title The core protein of a pestivirus protects the incoming virus against IFN-induced effectors
title_full The core protein of a pestivirus protects the incoming virus against IFN-induced effectors
title_fullStr The core protein of a pestivirus protects the incoming virus against IFN-induced effectors
title_full_unstemmed The core protein of a pestivirus protects the incoming virus against IFN-induced effectors
title_short The core protein of a pestivirus protects the incoming virus against IFN-induced effectors
title_sort core protein of a pestivirus protects the incoming virus against ifn-induced effectors
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5349576/
https://www.ncbi.nlm.nih.gov/pubmed/28290554
http://dx.doi.org/10.1038/srep44459
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