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Unique spatiotemporal requirements for intraflagellar transport genes during forebrain development
Primary cilia are organelles extended from virtually all cells and are required for the proper regulation of a number of canonical developmental pathways. The role in cortical development of proteins important for ciliary form and function is a relatively understudied area. Here we have taken a gene...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5349613/ https://www.ncbi.nlm.nih.gov/pubmed/28291836 http://dx.doi.org/10.1371/journal.pone.0173258 |
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author | Snedeker, John Schock, Elizabeth N. Struve, Jamie N. Chang, Ching-Fang Cionni, Megan Tran, Pamela V. Brugmann, Samantha A. Stottmann, Rolf W. |
author_facet | Snedeker, John Schock, Elizabeth N. Struve, Jamie N. Chang, Ching-Fang Cionni, Megan Tran, Pamela V. Brugmann, Samantha A. Stottmann, Rolf W. |
author_sort | Snedeker, John |
collection | PubMed |
description | Primary cilia are organelles extended from virtually all cells and are required for the proper regulation of a number of canonical developmental pathways. The role in cortical development of proteins important for ciliary form and function is a relatively understudied area. Here we have taken a genetic approach to define the role in forebrain development of three intraflagellar transport proteins known to be important for primary cilia function. We have genetically ablated Kif3a, Ift88, and Ttc21b in a series of specific spatiotemporal domains. The resulting phenotypes allow us to draw several conclusions. First, we conclude that the Ttc21b cortical phenotype is not due to the activity of Ttc21b within the brain itself. Secondly, some of the most striking phenotypes are from ablations in the neural crest cells and the adjacent surface ectoderm indicating that cilia transduce critical tissue—tissue interactions in the developing embryonic head. Finally, we note striking differences in phenotypes from ablations only one embryonic day apart, indicating very discrete spatiotemporal requirements for these three genes in cortical development. |
format | Online Article Text |
id | pubmed-5349613 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-53496132017-04-06 Unique spatiotemporal requirements for intraflagellar transport genes during forebrain development Snedeker, John Schock, Elizabeth N. Struve, Jamie N. Chang, Ching-Fang Cionni, Megan Tran, Pamela V. Brugmann, Samantha A. Stottmann, Rolf W. PLoS One Research Article Primary cilia are organelles extended from virtually all cells and are required for the proper regulation of a number of canonical developmental pathways. The role in cortical development of proteins important for ciliary form and function is a relatively understudied area. Here we have taken a genetic approach to define the role in forebrain development of three intraflagellar transport proteins known to be important for primary cilia function. We have genetically ablated Kif3a, Ift88, and Ttc21b in a series of specific spatiotemporal domains. The resulting phenotypes allow us to draw several conclusions. First, we conclude that the Ttc21b cortical phenotype is not due to the activity of Ttc21b within the brain itself. Secondly, some of the most striking phenotypes are from ablations in the neural crest cells and the adjacent surface ectoderm indicating that cilia transduce critical tissue—tissue interactions in the developing embryonic head. Finally, we note striking differences in phenotypes from ablations only one embryonic day apart, indicating very discrete spatiotemporal requirements for these three genes in cortical development. Public Library of Science 2017-03-14 /pmc/articles/PMC5349613/ /pubmed/28291836 http://dx.doi.org/10.1371/journal.pone.0173258 Text en © 2017 Snedeker et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Snedeker, John Schock, Elizabeth N. Struve, Jamie N. Chang, Ching-Fang Cionni, Megan Tran, Pamela V. Brugmann, Samantha A. Stottmann, Rolf W. Unique spatiotemporal requirements for intraflagellar transport genes during forebrain development |
title | Unique spatiotemporal requirements for intraflagellar transport genes during forebrain development |
title_full | Unique spatiotemporal requirements for intraflagellar transport genes during forebrain development |
title_fullStr | Unique spatiotemporal requirements for intraflagellar transport genes during forebrain development |
title_full_unstemmed | Unique spatiotemporal requirements for intraflagellar transport genes during forebrain development |
title_short | Unique spatiotemporal requirements for intraflagellar transport genes during forebrain development |
title_sort | unique spatiotemporal requirements for intraflagellar transport genes during forebrain development |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5349613/ https://www.ncbi.nlm.nih.gov/pubmed/28291836 http://dx.doi.org/10.1371/journal.pone.0173258 |
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