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Purification of cone outer segment for proteomic analysis on its membrane proteins in carp retina
Rods and cones are both photoreceptors in the retina, but they are different in many aspects including the light response characteristics and, for example, cell morphology and metabolism. These differences would be caused by differences in proteins expressed in rods and cones. To understand the mole...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5349680/ https://www.ncbi.nlm.nih.gov/pubmed/28291804 http://dx.doi.org/10.1371/journal.pone.0173908 |
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author | Fukagawa, Takashi Takafuji, Kazuaki Tachibanaki, Shuji Kawamura, Satoru |
author_facet | Fukagawa, Takashi Takafuji, Kazuaki Tachibanaki, Shuji Kawamura, Satoru |
author_sort | Fukagawa, Takashi |
collection | PubMed |
description | Rods and cones are both photoreceptors in the retina, but they are different in many aspects including the light response characteristics and, for example, cell morphology and metabolism. These differences would be caused by differences in proteins expressed in rods and cones. To understand the molecular bases of these differences between rods and cones, one of the ways is to compare proteins expressed in rods and cones, and to find those expressed specifically or dominantly. In the present study, we are interested in proteins in the outer segment (OS), the site responsible for generation of rod- or cone-characteristic light responses and also the site showing different morphology between rods and cones. For this, we established a method to purify the OS and the inner segment (IS) of rods and also of cones from purified carp rods and cones, respectively, using sucrose density gradient. In particular, we were interested in proteins tightly bound to the membranes of cone OS. To identify these proteins, we analyzed proteins in some selected regions of an SDS-gel of washed membranes of the OS and the IS obtained from both rods and cones, with Liquid Chromatography-tandem Mass Spectrometry (LC-MS/MS) using a protein database constructed from carp retina. By comparing the lists of the proteins found in the OS and the IS of both rods and cones, we found some proteins present in cone OS membranes specifically or dominantly, in addition to the proteins already known to be present specifically in cone OS. |
format | Online Article Text |
id | pubmed-5349680 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-53496802017-04-06 Purification of cone outer segment for proteomic analysis on its membrane proteins in carp retina Fukagawa, Takashi Takafuji, Kazuaki Tachibanaki, Shuji Kawamura, Satoru PLoS One Research Article Rods and cones are both photoreceptors in the retina, but they are different in many aspects including the light response characteristics and, for example, cell morphology and metabolism. These differences would be caused by differences in proteins expressed in rods and cones. To understand the molecular bases of these differences between rods and cones, one of the ways is to compare proteins expressed in rods and cones, and to find those expressed specifically or dominantly. In the present study, we are interested in proteins in the outer segment (OS), the site responsible for generation of rod- or cone-characteristic light responses and also the site showing different morphology between rods and cones. For this, we established a method to purify the OS and the inner segment (IS) of rods and also of cones from purified carp rods and cones, respectively, using sucrose density gradient. In particular, we were interested in proteins tightly bound to the membranes of cone OS. To identify these proteins, we analyzed proteins in some selected regions of an SDS-gel of washed membranes of the OS and the IS obtained from both rods and cones, with Liquid Chromatography-tandem Mass Spectrometry (LC-MS/MS) using a protein database constructed from carp retina. By comparing the lists of the proteins found in the OS and the IS of both rods and cones, we found some proteins present in cone OS membranes specifically or dominantly, in addition to the proteins already known to be present specifically in cone OS. Public Library of Science 2017-03-14 /pmc/articles/PMC5349680/ /pubmed/28291804 http://dx.doi.org/10.1371/journal.pone.0173908 Text en © 2017 Fukagawa et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Fukagawa, Takashi Takafuji, Kazuaki Tachibanaki, Shuji Kawamura, Satoru Purification of cone outer segment for proteomic analysis on its membrane proteins in carp retina |
title | Purification of cone outer segment for proteomic analysis on its membrane proteins in carp retina |
title_full | Purification of cone outer segment for proteomic analysis on its membrane proteins in carp retina |
title_fullStr | Purification of cone outer segment for proteomic analysis on its membrane proteins in carp retina |
title_full_unstemmed | Purification of cone outer segment for proteomic analysis on its membrane proteins in carp retina |
title_short | Purification of cone outer segment for proteomic analysis on its membrane proteins in carp retina |
title_sort | purification of cone outer segment for proteomic analysis on its membrane proteins in carp retina |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5349680/ https://www.ncbi.nlm.nih.gov/pubmed/28291804 http://dx.doi.org/10.1371/journal.pone.0173908 |
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