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Method to study cell migration under uniaxial compression

The chemical, physical, and mechanical properties of the extracellular environment have a strong effect on cell migration. Aspects such as pore size or stiffness of the matrix influence the selection of the mechanism used by cells to propel themselves, including by pseudopods or blebbing. How a cell...

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Detalles Bibliográficos
Autores principales: Srivastava, Nishit, Kay, Robert R., Kabla, Alexandre J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5349787/
https://www.ncbi.nlm.nih.gov/pubmed/28122819
http://dx.doi.org/10.1091/mbc.E16-08-0575
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author Srivastava, Nishit
Kay, Robert R.
Kabla, Alexandre J.
author_facet Srivastava, Nishit
Kay, Robert R.
Kabla, Alexandre J.
author_sort Srivastava, Nishit
collection PubMed
description The chemical, physical, and mechanical properties of the extracellular environment have a strong effect on cell migration. Aspects such as pore size or stiffness of the matrix influence the selection of the mechanism used by cells to propel themselves, including by pseudopods or blebbing. How a cell perceives its environment and how such a cue triggers a change in behavior are largely unknown, but mechanics is likely to be involved. Because mechanical conditions are often controlled by modifying the composition of the environment, separating chemical and physical contributions is difficult and requires multiple controls. Here we propose a simple method to impose a mechanical compression on individual cells without altering the composition of the matrix. Live imaging during compression provides accurate information about the cell's morphology and migratory phenotype. Using Dictyostelium as a model, we observe that a compression of the order of 500 Pa flattens the cells under gel by up to 50%. This uniaxial compression directly triggers a transition in the mode of migration from primarily pseudopodial to bleb driven in <30 s. This novel device is therefore capable of influencing cell migration in real time and offers a convenient approach with which to systematically study mechanotransduction in confined environments.
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spelling pubmed-53497872017-05-30 Method to study cell migration under uniaxial compression Srivastava, Nishit Kay, Robert R. Kabla, Alexandre J. Mol Biol Cell Articles The chemical, physical, and mechanical properties of the extracellular environment have a strong effect on cell migration. Aspects such as pore size or stiffness of the matrix influence the selection of the mechanism used by cells to propel themselves, including by pseudopods or blebbing. How a cell perceives its environment and how such a cue triggers a change in behavior are largely unknown, but mechanics is likely to be involved. Because mechanical conditions are often controlled by modifying the composition of the environment, separating chemical and physical contributions is difficult and requires multiple controls. Here we propose a simple method to impose a mechanical compression on individual cells without altering the composition of the matrix. Live imaging during compression provides accurate information about the cell's morphology and migratory phenotype. Using Dictyostelium as a model, we observe that a compression of the order of 500 Pa flattens the cells under gel by up to 50%. This uniaxial compression directly triggers a transition in the mode of migration from primarily pseudopodial to bleb driven in <30 s. This novel device is therefore capable of influencing cell migration in real time and offers a convenient approach with which to systematically study mechanotransduction in confined environments. The American Society for Cell Biology 2017-03-15 /pmc/articles/PMC5349787/ /pubmed/28122819 http://dx.doi.org/10.1091/mbc.E16-08-0575 Text en © 2017 Srivastava et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Articles
Srivastava, Nishit
Kay, Robert R.
Kabla, Alexandre J.
Method to study cell migration under uniaxial compression
title Method to study cell migration under uniaxial compression
title_full Method to study cell migration under uniaxial compression
title_fullStr Method to study cell migration under uniaxial compression
title_full_unstemmed Method to study cell migration under uniaxial compression
title_short Method to study cell migration under uniaxial compression
title_sort method to study cell migration under uniaxial compression
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5349787/
https://www.ncbi.nlm.nih.gov/pubmed/28122819
http://dx.doi.org/10.1091/mbc.E16-08-0575
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