Cargando…
Newcastle disease virus employs macropinocytosis and Rab5a-dependent intracellular trafficking to infect DF-1 cells
Oncolytic Newcastle disease virus (NDV) reportedly employs direct fusion of the viral envelope with the plasma membrane and caveolae-dependent endocytosis to enter cells. Here, we show that macropinocytosis and clathrin-mediated endocytosis are involved in NDV entry into a galline embryonic fibrobla...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5349901/ https://www.ncbi.nlm.nih.gov/pubmed/27861142 http://dx.doi.org/10.18632/oncotarget.13345 |
_version_ | 1782514553389580288 |
---|---|
author | Tan, Lei Zhang, Yuqiang Zhan, Yuan Yuan, Yanmei Sun, Yingjie Qiu, Xusheng Meng, Chunchun Song, Cuiping Liao, Ying Ding, Chan |
author_facet | Tan, Lei Zhang, Yuqiang Zhan, Yuan Yuan, Yanmei Sun, Yingjie Qiu, Xusheng Meng, Chunchun Song, Cuiping Liao, Ying Ding, Chan |
author_sort | Tan, Lei |
collection | PubMed |
description | Oncolytic Newcastle disease virus (NDV) reportedly employs direct fusion of the viral envelope with the plasma membrane and caveolae-dependent endocytosis to enter cells. Here, we show that macropinocytosis and clathrin-mediated endocytosis are involved in NDV entry into a galline embryonic fibroblast cell line. Upon specific inhibition of clathrin assembly, GTPase dynamin, Na(+)/H(+) exchangers, Ras-related C3 botulinum toxin substrate 1, p21 activated kinase 1 or protein kinase C, entry of NDV and its propagation were suppressed. NDV entry into cells triggers Rac1-Pak1 signaling and elicits actin rearrangement and plasma membrane ruffling. Moreover, NDV internalization within macropinosomes and trafficking involve Rab5a-positive vesicles. This is the first report demonstrating that NDV utilizes clathrin-mediated endocytosis and macropinocytosis as alternative endocytic pathways to enter cells. These findings shed new light on the molecular mechanisms underlying NDV entry into cells, and provide potential targets for NDV-mediated therapy in cancer. |
format | Online Article Text |
id | pubmed-5349901 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-53499012017-04-06 Newcastle disease virus employs macropinocytosis and Rab5a-dependent intracellular trafficking to infect DF-1 cells Tan, Lei Zhang, Yuqiang Zhan, Yuan Yuan, Yanmei Sun, Yingjie Qiu, Xusheng Meng, Chunchun Song, Cuiping Liao, Ying Ding, Chan Oncotarget Research Paper Oncolytic Newcastle disease virus (NDV) reportedly employs direct fusion of the viral envelope with the plasma membrane and caveolae-dependent endocytosis to enter cells. Here, we show that macropinocytosis and clathrin-mediated endocytosis are involved in NDV entry into a galline embryonic fibroblast cell line. Upon specific inhibition of clathrin assembly, GTPase dynamin, Na(+)/H(+) exchangers, Ras-related C3 botulinum toxin substrate 1, p21 activated kinase 1 or protein kinase C, entry of NDV and its propagation were suppressed. NDV entry into cells triggers Rac1-Pak1 signaling and elicits actin rearrangement and plasma membrane ruffling. Moreover, NDV internalization within macropinosomes and trafficking involve Rab5a-positive vesicles. This is the first report demonstrating that NDV utilizes clathrin-mediated endocytosis and macropinocytosis as alternative endocytic pathways to enter cells. These findings shed new light on the molecular mechanisms underlying NDV entry into cells, and provide potential targets for NDV-mediated therapy in cancer. Impact Journals LLC 2016-11-15 /pmc/articles/PMC5349901/ /pubmed/27861142 http://dx.doi.org/10.18632/oncotarget.13345 Text en Copyright: © 2016 Tan et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Tan, Lei Zhang, Yuqiang Zhan, Yuan Yuan, Yanmei Sun, Yingjie Qiu, Xusheng Meng, Chunchun Song, Cuiping Liao, Ying Ding, Chan Newcastle disease virus employs macropinocytosis and Rab5a-dependent intracellular trafficking to infect DF-1 cells |
title | Newcastle disease virus employs macropinocytosis and Rab5a-dependent intracellular trafficking to infect DF-1 cells |
title_full | Newcastle disease virus employs macropinocytosis and Rab5a-dependent intracellular trafficking to infect DF-1 cells |
title_fullStr | Newcastle disease virus employs macropinocytosis and Rab5a-dependent intracellular trafficking to infect DF-1 cells |
title_full_unstemmed | Newcastle disease virus employs macropinocytosis and Rab5a-dependent intracellular trafficking to infect DF-1 cells |
title_short | Newcastle disease virus employs macropinocytosis and Rab5a-dependent intracellular trafficking to infect DF-1 cells |
title_sort | newcastle disease virus employs macropinocytosis and rab5a-dependent intracellular trafficking to infect df-1 cells |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5349901/ https://www.ncbi.nlm.nih.gov/pubmed/27861142 http://dx.doi.org/10.18632/oncotarget.13345 |
work_keys_str_mv | AT tanlei newcastlediseasevirusemploysmacropinocytosisandrab5adependentintracellulartraffickingtoinfectdf1cells AT zhangyuqiang newcastlediseasevirusemploysmacropinocytosisandrab5adependentintracellulartraffickingtoinfectdf1cells AT zhanyuan newcastlediseasevirusemploysmacropinocytosisandrab5adependentintracellulartraffickingtoinfectdf1cells AT yuanyanmei newcastlediseasevirusemploysmacropinocytosisandrab5adependentintracellulartraffickingtoinfectdf1cells AT sunyingjie newcastlediseasevirusemploysmacropinocytosisandrab5adependentintracellulartraffickingtoinfectdf1cells AT qiuxusheng newcastlediseasevirusemploysmacropinocytosisandrab5adependentintracellulartraffickingtoinfectdf1cells AT mengchunchun newcastlediseasevirusemploysmacropinocytosisandrab5adependentintracellulartraffickingtoinfectdf1cells AT songcuiping newcastlediseasevirusemploysmacropinocytosisandrab5adependentintracellulartraffickingtoinfectdf1cells AT liaoying newcastlediseasevirusemploysmacropinocytosisandrab5adependentintracellulartraffickingtoinfectdf1cells AT dingchan newcastlediseasevirusemploysmacropinocytosisandrab5adependentintracellulartraffickingtoinfectdf1cells |