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Newcastle disease virus employs macropinocytosis and Rab5a-dependent intracellular trafficking to infect DF-1 cells

Oncolytic Newcastle disease virus (NDV) reportedly employs direct fusion of the viral envelope with the plasma membrane and caveolae-dependent endocytosis to enter cells. Here, we show that macropinocytosis and clathrin-mediated endocytosis are involved in NDV entry into a galline embryonic fibrobla...

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Autores principales: Tan, Lei, Zhang, Yuqiang, Zhan, Yuan, Yuan, Yanmei, Sun, Yingjie, Qiu, Xusheng, Meng, Chunchun, Song, Cuiping, Liao, Ying, Ding, Chan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5349901/
https://www.ncbi.nlm.nih.gov/pubmed/27861142
http://dx.doi.org/10.18632/oncotarget.13345
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author Tan, Lei
Zhang, Yuqiang
Zhan, Yuan
Yuan, Yanmei
Sun, Yingjie
Qiu, Xusheng
Meng, Chunchun
Song, Cuiping
Liao, Ying
Ding, Chan
author_facet Tan, Lei
Zhang, Yuqiang
Zhan, Yuan
Yuan, Yanmei
Sun, Yingjie
Qiu, Xusheng
Meng, Chunchun
Song, Cuiping
Liao, Ying
Ding, Chan
author_sort Tan, Lei
collection PubMed
description Oncolytic Newcastle disease virus (NDV) reportedly employs direct fusion of the viral envelope with the plasma membrane and caveolae-dependent endocytosis to enter cells. Here, we show that macropinocytosis and clathrin-mediated endocytosis are involved in NDV entry into a galline embryonic fibroblast cell line. Upon specific inhibition of clathrin assembly, GTPase dynamin, Na(+)/H(+) exchangers, Ras-related C3 botulinum toxin substrate 1, p21 activated kinase 1 or protein kinase C, entry of NDV and its propagation were suppressed. NDV entry into cells triggers Rac1-Pak1 signaling and elicits actin rearrangement and plasma membrane ruffling. Moreover, NDV internalization within macropinosomes and trafficking involve Rab5a-positive vesicles. This is the first report demonstrating that NDV utilizes clathrin-mediated endocytosis and macropinocytosis as alternative endocytic pathways to enter cells. These findings shed new light on the molecular mechanisms underlying NDV entry into cells, and provide potential targets for NDV-mediated therapy in cancer.
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spelling pubmed-53499012017-04-06 Newcastle disease virus employs macropinocytosis and Rab5a-dependent intracellular trafficking to infect DF-1 cells Tan, Lei Zhang, Yuqiang Zhan, Yuan Yuan, Yanmei Sun, Yingjie Qiu, Xusheng Meng, Chunchun Song, Cuiping Liao, Ying Ding, Chan Oncotarget Research Paper Oncolytic Newcastle disease virus (NDV) reportedly employs direct fusion of the viral envelope with the plasma membrane and caveolae-dependent endocytosis to enter cells. Here, we show that macropinocytosis and clathrin-mediated endocytosis are involved in NDV entry into a galline embryonic fibroblast cell line. Upon specific inhibition of clathrin assembly, GTPase dynamin, Na(+)/H(+) exchangers, Ras-related C3 botulinum toxin substrate 1, p21 activated kinase 1 or protein kinase C, entry of NDV and its propagation were suppressed. NDV entry into cells triggers Rac1-Pak1 signaling and elicits actin rearrangement and plasma membrane ruffling. Moreover, NDV internalization within macropinosomes and trafficking involve Rab5a-positive vesicles. This is the first report demonstrating that NDV utilizes clathrin-mediated endocytosis and macropinocytosis as alternative endocytic pathways to enter cells. These findings shed new light on the molecular mechanisms underlying NDV entry into cells, and provide potential targets for NDV-mediated therapy in cancer. Impact Journals LLC 2016-11-15 /pmc/articles/PMC5349901/ /pubmed/27861142 http://dx.doi.org/10.18632/oncotarget.13345 Text en Copyright: © 2016 Tan et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Tan, Lei
Zhang, Yuqiang
Zhan, Yuan
Yuan, Yanmei
Sun, Yingjie
Qiu, Xusheng
Meng, Chunchun
Song, Cuiping
Liao, Ying
Ding, Chan
Newcastle disease virus employs macropinocytosis and Rab5a-dependent intracellular trafficking to infect DF-1 cells
title Newcastle disease virus employs macropinocytosis and Rab5a-dependent intracellular trafficking to infect DF-1 cells
title_full Newcastle disease virus employs macropinocytosis and Rab5a-dependent intracellular trafficking to infect DF-1 cells
title_fullStr Newcastle disease virus employs macropinocytosis and Rab5a-dependent intracellular trafficking to infect DF-1 cells
title_full_unstemmed Newcastle disease virus employs macropinocytosis and Rab5a-dependent intracellular trafficking to infect DF-1 cells
title_short Newcastle disease virus employs macropinocytosis and Rab5a-dependent intracellular trafficking to infect DF-1 cells
title_sort newcastle disease virus employs macropinocytosis and rab5a-dependent intracellular trafficking to infect df-1 cells
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5349901/
https://www.ncbi.nlm.nih.gov/pubmed/27861142
http://dx.doi.org/10.18632/oncotarget.13345
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