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Gli1 promotes colorectal cancer metastasis in a Foxm1-dependent manner by activating EMT and PI3K-AKT signaling

Colorectal cancer(CRC) is one of the most commonly diagnosed cancers in human beings and metastasis is the main death reason. Recently, Gli1 has been reported to be a key regulator of various cancer biologies and genes expressions. However, the detailed molecular mechanism of Gli1 in CRC metastasis...

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Autores principales: Zhang, Chuan, Wang, Yong, Feng, YiFei, Zhang, Yue, Ji, Bing, Wang, Sen, Sun, Ye, Zhu, Chunyan, Zhang, Dongsheng, Sun, Yueming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5349902/
https://www.ncbi.nlm.nih.gov/pubmed/27863385
http://dx.doi.org/10.18632/oncotarget.13348
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author Zhang, Chuan
Wang, Yong
Feng, YiFei
Zhang, Yue
Ji, Bing
Wang, Sen
Sun, Ye
Zhu, Chunyan
Zhang, Dongsheng
Sun, Yueming
author_facet Zhang, Chuan
Wang, Yong
Feng, YiFei
Zhang, Yue
Ji, Bing
Wang, Sen
Sun, Ye
Zhu, Chunyan
Zhang, Dongsheng
Sun, Yueming
author_sort Zhang, Chuan
collection PubMed
description Colorectal cancer(CRC) is one of the most commonly diagnosed cancers in human beings and metastasis is the main death reason. Recently, Gli1 has been reported to be a key regulator of various cancer biologies and genes expressions. However, the detailed molecular mechanism of Gli1 in CRC metastasis remains largely unknown. In this study, we aimed to investigate the role of Gli1 in CRC metastasis. We used qRT-PCR, Immunohistochemistry and Western blot to test the expression levels of Gli1, Foxm1 and other target genes in the tissues and cells; Lentivirus stable transfection to change the expression levels of Gli1 and Foxm1; Wound-healing, cell invasion, migration assays and tail vein metastatic assay to test the role of Gli1 in CRC metastasis in vitro and vivo. We demonstrated that Gli1 was significantly overexpressed in colorectal cancer tissues and cells. Foxm1 level had a positive correlation with Gli1. Furthermore, we found that Gli1 promotes colorectal cancer cells metastasis in a Foxm1-dependent manner by activating EMT and PI3K-AKT signaling. Thus, we proved that Gli1 plays important role in CRC metastasis and provided a new visual field on the therapy of CRC metastasis.
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spelling pubmed-53499022017-04-06 Gli1 promotes colorectal cancer metastasis in a Foxm1-dependent manner by activating EMT and PI3K-AKT signaling Zhang, Chuan Wang, Yong Feng, YiFei Zhang, Yue Ji, Bing Wang, Sen Sun, Ye Zhu, Chunyan Zhang, Dongsheng Sun, Yueming Oncotarget Research Paper Colorectal cancer(CRC) is one of the most commonly diagnosed cancers in human beings and metastasis is the main death reason. Recently, Gli1 has been reported to be a key regulator of various cancer biologies and genes expressions. However, the detailed molecular mechanism of Gli1 in CRC metastasis remains largely unknown. In this study, we aimed to investigate the role of Gli1 in CRC metastasis. We used qRT-PCR, Immunohistochemistry and Western blot to test the expression levels of Gli1, Foxm1 and other target genes in the tissues and cells; Lentivirus stable transfection to change the expression levels of Gli1 and Foxm1; Wound-healing, cell invasion, migration assays and tail vein metastatic assay to test the role of Gli1 in CRC metastasis in vitro and vivo. We demonstrated that Gli1 was significantly overexpressed in colorectal cancer tissues and cells. Foxm1 level had a positive correlation with Gli1. Furthermore, we found that Gli1 promotes colorectal cancer cells metastasis in a Foxm1-dependent manner by activating EMT and PI3K-AKT signaling. Thus, we proved that Gli1 plays important role in CRC metastasis and provided a new visual field on the therapy of CRC metastasis. Impact Journals LLC 2016-11-15 /pmc/articles/PMC5349902/ /pubmed/27863385 http://dx.doi.org/10.18632/oncotarget.13348 Text en Copyright: © 2016 Zhang et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Zhang, Chuan
Wang, Yong
Feng, YiFei
Zhang, Yue
Ji, Bing
Wang, Sen
Sun, Ye
Zhu, Chunyan
Zhang, Dongsheng
Sun, Yueming
Gli1 promotes colorectal cancer metastasis in a Foxm1-dependent manner by activating EMT and PI3K-AKT signaling
title Gli1 promotes colorectal cancer metastasis in a Foxm1-dependent manner by activating EMT and PI3K-AKT signaling
title_full Gli1 promotes colorectal cancer metastasis in a Foxm1-dependent manner by activating EMT and PI3K-AKT signaling
title_fullStr Gli1 promotes colorectal cancer metastasis in a Foxm1-dependent manner by activating EMT and PI3K-AKT signaling
title_full_unstemmed Gli1 promotes colorectal cancer metastasis in a Foxm1-dependent manner by activating EMT and PI3K-AKT signaling
title_short Gli1 promotes colorectal cancer metastasis in a Foxm1-dependent manner by activating EMT and PI3K-AKT signaling
title_sort gli1 promotes colorectal cancer metastasis in a foxm1-dependent manner by activating emt and pi3k-akt signaling
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5349902/
https://www.ncbi.nlm.nih.gov/pubmed/27863385
http://dx.doi.org/10.18632/oncotarget.13348
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