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microRNA-802 inhibits epithelial-mesenchymal transition through targeting flotillin-2 in human prostate cancer
miRNAs are a class of non-coding RNAs that exert critical roles in various biological processes. The aim of the present study was to identify the functional roles of miR-802 in regulating epithelial–mesenchymal transition (EMT) in prostate cancer (PCa). miR-802 expression was detected in 73 pairs of...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5350603/ https://www.ncbi.nlm.nih.gov/pubmed/28188157 http://dx.doi.org/10.1042/BSR20160521 |
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author | Wang, Dawei Lu, Guoliang Shao, Yuan Xu, Da |
author_facet | Wang, Dawei Lu, Guoliang Shao, Yuan Xu, Da |
author_sort | Wang, Dawei |
collection | PubMed |
description | miRNAs are a class of non-coding RNAs that exert critical roles in various biological processes. The aim of the present study was to identify the functional roles of miR-802 in regulating epithelial–mesenchymal transition (EMT) in prostate cancer (PCa). miR-802 expression was detected in 73 pairs of PCa samples and PCa cell lines (PC3 and DU145 cells) by qRT-PCR. Cell proliferation was detected using MTT assay, and cell apoptosis was evaluated using flow cytometry. Transwell assay was conducted to investigate cell migration and invasion. Expression analysis of a set of EMT markers was performed to explore whether miR-802 is involved in EMT program. Xenograft model was established to investigate the function of miR-802 in carcinogenesis in vivo. The direct regulation of Flotillin-2 (Flot2) by miR-802 was identified using luciferase reporter assay. miR-802 was remarkably down-regulated in PCa tissues and cell lines. Gain-of-function trails showed that miR-802 serves as an ‘oncosuppressor’ in PCa through inhibiting cell proliferation and promoting cell apoptosis in vitro. Overexpression of miR-802 significantly suppressed in vivo PCa tumor growth. Luciferase reporter analysis identified Flot2 as a direct target of miR-802 in PCa cells. Overexpressed miR-802 significantly suppressed EMT, migration and invasion in PCa cells by regulating Flot2. We identified miR-802 as a novel tumor suppressor in PCa progression and elucidated a novel mechanism of the miR-802/Flot2 axis in the regulation of EMT, which may be a potential therapeutic target. |
format | Online Article Text |
id | pubmed-5350603 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-53506032017-04-30 microRNA-802 inhibits epithelial-mesenchymal transition through targeting flotillin-2 in human prostate cancer Wang, Dawei Lu, Guoliang Shao, Yuan Xu, Da Biosci Rep Research Articles miRNAs are a class of non-coding RNAs that exert critical roles in various biological processes. The aim of the present study was to identify the functional roles of miR-802 in regulating epithelial–mesenchymal transition (EMT) in prostate cancer (PCa). miR-802 expression was detected in 73 pairs of PCa samples and PCa cell lines (PC3 and DU145 cells) by qRT-PCR. Cell proliferation was detected using MTT assay, and cell apoptosis was evaluated using flow cytometry. Transwell assay was conducted to investigate cell migration and invasion. Expression analysis of a set of EMT markers was performed to explore whether miR-802 is involved in EMT program. Xenograft model was established to investigate the function of miR-802 in carcinogenesis in vivo. The direct regulation of Flotillin-2 (Flot2) by miR-802 was identified using luciferase reporter assay. miR-802 was remarkably down-regulated in PCa tissues and cell lines. Gain-of-function trails showed that miR-802 serves as an ‘oncosuppressor’ in PCa through inhibiting cell proliferation and promoting cell apoptosis in vitro. Overexpression of miR-802 significantly suppressed in vivo PCa tumor growth. Luciferase reporter analysis identified Flot2 as a direct target of miR-802 in PCa cells. Overexpressed miR-802 significantly suppressed EMT, migration and invasion in PCa cells by regulating Flot2. We identified miR-802 as a novel tumor suppressor in PCa progression and elucidated a novel mechanism of the miR-802/Flot2 axis in the regulation of EMT, which may be a potential therapeutic target. Portland Press Ltd. 2017-03-15 /pmc/articles/PMC5350603/ /pubmed/28188157 http://dx.doi.org/10.1042/BSR20160521 Text en © 2017 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Articles Wang, Dawei Lu, Guoliang Shao, Yuan Xu, Da microRNA-802 inhibits epithelial-mesenchymal transition through targeting flotillin-2 in human prostate cancer |
title | microRNA-802 inhibits epithelial-mesenchymal transition through targeting flotillin-2 in human prostate cancer |
title_full | microRNA-802 inhibits epithelial-mesenchymal transition through targeting flotillin-2 in human prostate cancer |
title_fullStr | microRNA-802 inhibits epithelial-mesenchymal transition through targeting flotillin-2 in human prostate cancer |
title_full_unstemmed | microRNA-802 inhibits epithelial-mesenchymal transition through targeting flotillin-2 in human prostate cancer |
title_short | microRNA-802 inhibits epithelial-mesenchymal transition through targeting flotillin-2 in human prostate cancer |
title_sort | microrna-802 inhibits epithelial-mesenchymal transition through targeting flotillin-2 in human prostate cancer |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5350603/ https://www.ncbi.nlm.nih.gov/pubmed/28188157 http://dx.doi.org/10.1042/BSR20160521 |
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