Cargando…

microRNA-802 inhibits epithelial-mesenchymal transition through targeting flotillin-2 in human prostate cancer

miRNAs are a class of non-coding RNAs that exert critical roles in various biological processes. The aim of the present study was to identify the functional roles of miR-802 in regulating epithelial–mesenchymal transition (EMT) in prostate cancer (PCa). miR-802 expression was detected in 73 pairs of...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Dawei, Lu, Guoliang, Shao, Yuan, Xu, Da
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5350603/
https://www.ncbi.nlm.nih.gov/pubmed/28188157
http://dx.doi.org/10.1042/BSR20160521
_version_ 1782514696090288128
author Wang, Dawei
Lu, Guoliang
Shao, Yuan
Xu, Da
author_facet Wang, Dawei
Lu, Guoliang
Shao, Yuan
Xu, Da
author_sort Wang, Dawei
collection PubMed
description miRNAs are a class of non-coding RNAs that exert critical roles in various biological processes. The aim of the present study was to identify the functional roles of miR-802 in regulating epithelial–mesenchymal transition (EMT) in prostate cancer (PCa). miR-802 expression was detected in 73 pairs of PCa samples and PCa cell lines (PC3 and DU145 cells) by qRT-PCR. Cell proliferation was detected using MTT assay, and cell apoptosis was evaluated using flow cytometry. Transwell assay was conducted to investigate cell migration and invasion. Expression analysis of a set of EMT markers was performed to explore whether miR-802 is involved in EMT program. Xenograft model was established to investigate the function of miR-802 in carcinogenesis in vivo. The direct regulation of Flotillin-2 (Flot2) by miR-802 was identified using luciferase reporter assay. miR-802 was remarkably down-regulated in PCa tissues and cell lines. Gain-of-function trails showed that miR-802 serves as an ‘oncosuppressor’ in PCa through inhibiting cell proliferation and promoting cell apoptosis in vitro. Overexpression of miR-802 significantly suppressed in vivo PCa tumor growth. Luciferase reporter analysis identified Flot2 as a direct target of miR-802 in PCa cells. Overexpressed miR-802 significantly suppressed EMT, migration and invasion in PCa cells by regulating Flot2. We identified miR-802 as a novel tumor suppressor in PCa progression and elucidated a novel mechanism of the miR-802/Flot2 axis in the regulation of EMT, which may be a potential therapeutic target.
format Online
Article
Text
id pubmed-5350603
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Portland Press Ltd.
record_format MEDLINE/PubMed
spelling pubmed-53506032017-04-30 microRNA-802 inhibits epithelial-mesenchymal transition through targeting flotillin-2 in human prostate cancer Wang, Dawei Lu, Guoliang Shao, Yuan Xu, Da Biosci Rep Research Articles miRNAs are a class of non-coding RNAs that exert critical roles in various biological processes. The aim of the present study was to identify the functional roles of miR-802 in regulating epithelial–mesenchymal transition (EMT) in prostate cancer (PCa). miR-802 expression was detected in 73 pairs of PCa samples and PCa cell lines (PC3 and DU145 cells) by qRT-PCR. Cell proliferation was detected using MTT assay, and cell apoptosis was evaluated using flow cytometry. Transwell assay was conducted to investigate cell migration and invasion. Expression analysis of a set of EMT markers was performed to explore whether miR-802 is involved in EMT program. Xenograft model was established to investigate the function of miR-802 in carcinogenesis in vivo. The direct regulation of Flotillin-2 (Flot2) by miR-802 was identified using luciferase reporter assay. miR-802 was remarkably down-regulated in PCa tissues and cell lines. Gain-of-function trails showed that miR-802 serves as an ‘oncosuppressor’ in PCa through inhibiting cell proliferation and promoting cell apoptosis in vitro. Overexpression of miR-802 significantly suppressed in vivo PCa tumor growth. Luciferase reporter analysis identified Flot2 as a direct target of miR-802 in PCa cells. Overexpressed miR-802 significantly suppressed EMT, migration and invasion in PCa cells by regulating Flot2. We identified miR-802 as a novel tumor suppressor in PCa progression and elucidated a novel mechanism of the miR-802/Flot2 axis in the regulation of EMT, which may be a potential therapeutic target. Portland Press Ltd. 2017-03-15 /pmc/articles/PMC5350603/ /pubmed/28188157 http://dx.doi.org/10.1042/BSR20160521 Text en © 2017 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Articles
Wang, Dawei
Lu, Guoliang
Shao, Yuan
Xu, Da
microRNA-802 inhibits epithelial-mesenchymal transition through targeting flotillin-2 in human prostate cancer
title microRNA-802 inhibits epithelial-mesenchymal transition through targeting flotillin-2 in human prostate cancer
title_full microRNA-802 inhibits epithelial-mesenchymal transition through targeting flotillin-2 in human prostate cancer
title_fullStr microRNA-802 inhibits epithelial-mesenchymal transition through targeting flotillin-2 in human prostate cancer
title_full_unstemmed microRNA-802 inhibits epithelial-mesenchymal transition through targeting flotillin-2 in human prostate cancer
title_short microRNA-802 inhibits epithelial-mesenchymal transition through targeting flotillin-2 in human prostate cancer
title_sort microrna-802 inhibits epithelial-mesenchymal transition through targeting flotillin-2 in human prostate cancer
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5350603/
https://www.ncbi.nlm.nih.gov/pubmed/28188157
http://dx.doi.org/10.1042/BSR20160521
work_keys_str_mv AT wangdawei microrna802inhibitsepithelialmesenchymaltransitionthroughtargetingflotillin2inhumanprostatecancer
AT luguoliang microrna802inhibitsepithelialmesenchymaltransitionthroughtargetingflotillin2inhumanprostatecancer
AT shaoyuan microrna802inhibitsepithelialmesenchymaltransitionthroughtargetingflotillin2inhumanprostatecancer
AT xuda microrna802inhibitsepithelialmesenchymaltransitionthroughtargetingflotillin2inhumanprostatecancer