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Neuroprotective effect of Clerodendrum serratum Linn. leaves extract against acute restraint stress-induced depressive-like behavioral symptoms in adult mice
OBJECTIVE: The objective of this study was to study the effect of ethanol extract of Clerodendrum serratum (EECS) Linn. on acute restraint stress (ARS)-induced depressive-like behavior and biochemical alterations in mice. MATERIALS AND METHODS: Ethyl acetate and n-butanol fractions of EECS were anal...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Medknow Publications & Media Pvt Ltd
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5351235/ https://www.ncbi.nlm.nih.gov/pubmed/28458420 http://dx.doi.org/10.4103/0253-7613.201028 |
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author | Vazhayil, Babitha K. Rajagopal, Shanmuga Sundaram Thangavelu, Thiyagarajan Swaminathan, Gomathi Rajagounder, Elavarasi |
author_facet | Vazhayil, Babitha K. Rajagopal, Shanmuga Sundaram Thangavelu, Thiyagarajan Swaminathan, Gomathi Rajagounder, Elavarasi |
author_sort | Vazhayil, Babitha K. |
collection | PubMed |
description | OBJECTIVE: The objective of this study was to study the effect of ethanol extract of Clerodendrum serratum (EECS) Linn. on acute restraint stress (ARS)-induced depressive-like behavior and biochemical alterations in mice. MATERIALS AND METHODS: Ethyl acetate and n-butanol fractions of EECS were analytically characterized for the flavonoid components, apigenin (API) and luteolin (LUT) by reverse-phase high-performance liquid chromatography. Behavioral tests, namely, forced-swim test and tail-suspension test were performed for assessing antidepressant-like effect and anxiolytic activity in mice. Oxidative stress parameters and biochemical alterations in mice brain tissue were also performed. STATISTICAL ANALYSIS: Expression of data was done as mean ± standard error of mean. The normally distributed data were subjected to two-way ANOVA followed by Dunnett's test. P < 0.05 was considered statistically significant. RESULTS: The study showed that flavonoids, API and LUT were present in ethyl acetate and n-butanol fractions of EECS, which significantly reversed ARS-induced depressive-like behavior without affecting locomotion. EECS also attenuated oxidative damage caused by ARS. The level of norepinephrine and 5-hydroxytryptamine was also significantly restored by pretreatment with EECS for 7 days. CONCLUSION: EECS significantly alleviated ARS-induced depressive-like behavior without affecting locomotion. |
format | Online Article Text |
id | pubmed-5351235 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-53512352017-04-28 Neuroprotective effect of Clerodendrum serratum Linn. leaves extract against acute restraint stress-induced depressive-like behavioral symptoms in adult mice Vazhayil, Babitha K. Rajagopal, Shanmuga Sundaram Thangavelu, Thiyagarajan Swaminathan, Gomathi Rajagounder, Elavarasi Indian J Pharmacol Research Article OBJECTIVE: The objective of this study was to study the effect of ethanol extract of Clerodendrum serratum (EECS) Linn. on acute restraint stress (ARS)-induced depressive-like behavior and biochemical alterations in mice. MATERIALS AND METHODS: Ethyl acetate and n-butanol fractions of EECS were analytically characterized for the flavonoid components, apigenin (API) and luteolin (LUT) by reverse-phase high-performance liquid chromatography. Behavioral tests, namely, forced-swim test and tail-suspension test were performed for assessing antidepressant-like effect and anxiolytic activity in mice. Oxidative stress parameters and biochemical alterations in mice brain tissue were also performed. STATISTICAL ANALYSIS: Expression of data was done as mean ± standard error of mean. The normally distributed data were subjected to two-way ANOVA followed by Dunnett's test. P < 0.05 was considered statistically significant. RESULTS: The study showed that flavonoids, API and LUT were present in ethyl acetate and n-butanol fractions of EECS, which significantly reversed ARS-induced depressive-like behavior without affecting locomotion. EECS also attenuated oxidative damage caused by ARS. The level of norepinephrine and 5-hydroxytryptamine was also significantly restored by pretreatment with EECS for 7 days. CONCLUSION: EECS significantly alleviated ARS-induced depressive-like behavior without affecting locomotion. Medknow Publications & Media Pvt Ltd 2017 /pmc/articles/PMC5351235/ /pubmed/28458420 http://dx.doi.org/10.4103/0253-7613.201028 Text en Copyright: © 2017 Indian Journal of Pharmacology http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. |
spellingShingle | Research Article Vazhayil, Babitha K. Rajagopal, Shanmuga Sundaram Thangavelu, Thiyagarajan Swaminathan, Gomathi Rajagounder, Elavarasi Neuroprotective effect of Clerodendrum serratum Linn. leaves extract against acute restraint stress-induced depressive-like behavioral symptoms in adult mice |
title | Neuroprotective effect of Clerodendrum serratum Linn. leaves extract against acute restraint stress-induced depressive-like behavioral symptoms in adult mice |
title_full | Neuroprotective effect of Clerodendrum serratum Linn. leaves extract against acute restraint stress-induced depressive-like behavioral symptoms in adult mice |
title_fullStr | Neuroprotective effect of Clerodendrum serratum Linn. leaves extract against acute restraint stress-induced depressive-like behavioral symptoms in adult mice |
title_full_unstemmed | Neuroprotective effect of Clerodendrum serratum Linn. leaves extract against acute restraint stress-induced depressive-like behavioral symptoms in adult mice |
title_short | Neuroprotective effect of Clerodendrum serratum Linn. leaves extract against acute restraint stress-induced depressive-like behavioral symptoms in adult mice |
title_sort | neuroprotective effect of clerodendrum serratum linn. leaves extract against acute restraint stress-induced depressive-like behavioral symptoms in adult mice |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5351235/ https://www.ncbi.nlm.nih.gov/pubmed/28458420 http://dx.doi.org/10.4103/0253-7613.201028 |
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