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Highly efficient mesophyll protoplast isolation and PEG-mediated transient gene expression for rapid and large-scale gene characterization in cassava (Manihot esculenta Crantz)

BACKGROUND: Cassava (Manihot esculenta Crantz) is a major crop extensively cultivated in the tropics as both an important source of calories and a promising source for biofuel production. Although stable gene expression have been used for transgenic breeding and gene function study, a quick, easy an...

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Detalles Bibliográficos
Autores principales: Wu, Jun-Zheng, Liu, Qin, Geng, Xiao-Shan, Li, Kai-Mian, Luo, Li-Juan, Liu, Jin-Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5351281/
https://www.ncbi.nlm.nih.gov/pubmed/28292294
http://dx.doi.org/10.1186/s12896-017-0349-2