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Highly efficient mesophyll protoplast isolation and PEG-mediated transient gene expression for rapid and large-scale gene characterization in cassava (Manihot esculenta Crantz)
BACKGROUND: Cassava (Manihot esculenta Crantz) is a major crop extensively cultivated in the tropics as both an important source of calories and a promising source for biofuel production. Although stable gene expression have been used for transgenic breeding and gene function study, a quick, easy an...
Autores principales: | Wu, Jun-Zheng, Liu, Qin, Geng, Xiao-Shan, Li, Kai-Mian, Luo, Li-Juan, Liu, Jin-Ping |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5351281/ https://www.ncbi.nlm.nih.gov/pubmed/28292294 http://dx.doi.org/10.1186/s12896-017-0349-2 |
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