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mir-218-2 promotes glioblastomas growth, invasion and drug resistance by targeting CDC27
Glioma has become a significant global health problem with substantial morbidity and mortality, underscoring the importance of elucidating its underlying molecular mechanisms. Recent studies have identified mir-218 as an anti-oncogene; however, the specific functions of mir-218-1 and mir-218-2 remai...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5351633/ https://www.ncbi.nlm.nih.gov/pubmed/27974673 http://dx.doi.org/10.18632/oncotarget.13850 |
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author | Feng, Zhuoying Zhang, Luping Zhou, Junchen Zhou, Shuai li, Li Guo, Xuyan Feng, Guoying Ma, Ze Huang, Wenhua Huang, Fei |
author_facet | Feng, Zhuoying Zhang, Luping Zhou, Junchen Zhou, Shuai li, Li Guo, Xuyan Feng, Guoying Ma, Ze Huang, Wenhua Huang, Fei |
author_sort | Feng, Zhuoying |
collection | PubMed |
description | Glioma has become a significant global health problem with substantial morbidity and mortality, underscoring the importance of elucidating its underlying molecular mechanisms. Recent studies have identified mir-218 as an anti-oncogene; however, the specific functions of mir-218-1 and mir-218-2 remain unknown, especially the latter. The objective of this study was to further investigate the role of mir-218-2 in glioma. Our results indicated that mir-218-2 is highly overexpressed in glioma. Furthermore, we showed that mir-218-2 is positively correlated with the growth, invasion, migration, and drug susceptibility (to β-lapachone) of glioma cells. In vitro, the overexpression of mir-218-2 promoted glioma cell proliferation, invasion, and migration. In addition, the overexpression of mir-218-2 in vivo was found to increase glioma tumor growth. Accordingly, the inhibition of mir-218-2 resulted in the opposite effects. Cell division cycle 27 (CDC27), the downstream target of mir-218-2, is involved in the regulation of glioma cells. Our results indicate that the overexpression of CDC27 counteracted the function of mir-218-2 in glioma cells. These novel findings provide new insight in the application of mir-218-2 as a potential glioma treatment. |
format | Online Article Text |
id | pubmed-5351633 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-53516332017-04-13 mir-218-2 promotes glioblastomas growth, invasion and drug resistance by targeting CDC27 Feng, Zhuoying Zhang, Luping Zhou, Junchen Zhou, Shuai li, Li Guo, Xuyan Feng, Guoying Ma, Ze Huang, Wenhua Huang, Fei Oncotarget Research Paper Glioma has become a significant global health problem with substantial morbidity and mortality, underscoring the importance of elucidating its underlying molecular mechanisms. Recent studies have identified mir-218 as an anti-oncogene; however, the specific functions of mir-218-1 and mir-218-2 remain unknown, especially the latter. The objective of this study was to further investigate the role of mir-218-2 in glioma. Our results indicated that mir-218-2 is highly overexpressed in glioma. Furthermore, we showed that mir-218-2 is positively correlated with the growth, invasion, migration, and drug susceptibility (to β-lapachone) of glioma cells. In vitro, the overexpression of mir-218-2 promoted glioma cell proliferation, invasion, and migration. In addition, the overexpression of mir-218-2 in vivo was found to increase glioma tumor growth. Accordingly, the inhibition of mir-218-2 resulted in the opposite effects. Cell division cycle 27 (CDC27), the downstream target of mir-218-2, is involved in the regulation of glioma cells. Our results indicate that the overexpression of CDC27 counteracted the function of mir-218-2 in glioma cells. These novel findings provide new insight in the application of mir-218-2 as a potential glioma treatment. Impact Journals LLC 2016-12-10 /pmc/articles/PMC5351633/ /pubmed/27974673 http://dx.doi.org/10.18632/oncotarget.13850 Text en Copyright: © 2017 Feng et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Feng, Zhuoying Zhang, Luping Zhou, Junchen Zhou, Shuai li, Li Guo, Xuyan Feng, Guoying Ma, Ze Huang, Wenhua Huang, Fei mir-218-2 promotes glioblastomas growth, invasion and drug resistance by targeting CDC27 |
title | mir-218-2 promotes glioblastomas growth, invasion and drug resistance by targeting CDC27 |
title_full | mir-218-2 promotes glioblastomas growth, invasion and drug resistance by targeting CDC27 |
title_fullStr | mir-218-2 promotes glioblastomas growth, invasion and drug resistance by targeting CDC27 |
title_full_unstemmed | mir-218-2 promotes glioblastomas growth, invasion and drug resistance by targeting CDC27 |
title_short | mir-218-2 promotes glioblastomas growth, invasion and drug resistance by targeting CDC27 |
title_sort | mir-218-2 promotes glioblastomas growth, invasion and drug resistance by targeting cdc27 |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5351633/ https://www.ncbi.nlm.nih.gov/pubmed/27974673 http://dx.doi.org/10.18632/oncotarget.13850 |
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