Indole-fused benzooxazepines: a new structural class of anticancer agents

AIM: A new series of compounds (1a–16a) bearing indole-fused benzooxazepine was synthesized, characterized and evaluated for anticancer activity. MATERIALS & METHODS: In this study, all the synthesized compounds were screened via in vitro anticancer testing on Hep-G2 cancer cell line. A computational study was carried out on cancer-related targets including IL-2, IL-6, COX-2 Caspase-3 and Caspase-8. RESULTS: Some of the synthesized compounds effectively controlled the growth of cancerous cells. CONCLUSION: The most active compounds – 6a, 10a, 13a, 14a and 15a – exemplify notable anticancer profile with GI(50) <10 μg/ml. Preliminary structure–activity relationship among the tested compounds can produce an assumption that the electronegative groups at phenyl ring attached with indole-fused benzooxazepine are instrumental for the activity. Molecular docking study showed crucial hydrogen bond and π–π stacking interactions, with good ADMET profiling and molecular dynamic simulation.