Cargando…
Association of non-synonymous variants in WIPF3 and LIPA genes with abdominal aortic aneurysm: an autopsy study
BACKGROUND: Abdominal aortic aneurysm (AAA) is a multifactorial disease with strong genetic components. Various genetic loci have been associated with clinical AAA, but few studies have investigated pathological AAA, an intermediate phenotype of the disease. METHODS: We examined 2263 consecutive aut...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Science Press
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5351826/ https://www.ncbi.nlm.nih.gov/pubmed/28321238 http://dx.doi.org/10.11909/j.issn.1671-5411.2016.12.003 |
_version_ | 1782514831872491520 |
---|---|
author | Maeda, Yuko Sato, Noriko Naka-Mieno, Makiko Mori, Seijiro Arai, Tomio Tanaka, Masashi Muramatsu, Masaaki Sawabe, Motoji |
author_facet | Maeda, Yuko Sato, Noriko Naka-Mieno, Makiko Mori, Seijiro Arai, Tomio Tanaka, Masashi Muramatsu, Masaaki Sawabe, Motoji |
author_sort | Maeda, Yuko |
collection | PubMed |
description | BACKGROUND: Abdominal aortic aneurysm (AAA) is a multifactorial disease with strong genetic components. Various genetic loci have been associated with clinical AAA, but few studies have investigated pathological AAA, an intermediate phenotype of the disease. METHODS: We examined 2263 consecutive autopsies of older Japanese subjects from a study on geriatric diseases in Japanese individuals (The JG-SNP study). The presence of AAA was determined with a pathological diagnosis during autopsy. Single nucleotide variants (SNVs) associated with AAA were determined with an Illumina HumanExome Beadchip array. Logistic regression analyses were performed to determine genetic associations. Age, gender, and other risk factors of AAA were analyzed as covariates. RESULTS: 118 subjects with AAA and 2145 subjects without AAA were analyzed in a case-control setting. No variants reached significance after applying the Bonferroni correction (P < 2.05×10(−6)). The strongest associations were found with rs3750092 (p.E321G, OR: 0.36, 95% CI: 0.24–0.56, P = 6.09 × 10(−6)), a variant in the WAS/WASL interacting protein family 3 (WIPF3), and with rs1051338 (p.T16P, OR: 2.50, 95% CI: 1.70–3.66, P = 2.79 × 10(−6)) and rs2246942 (intronic, OR: 2.32, 95% CI: 1.58–3.41, P = 1.61 × 10(−5)), variants in the lysosomal acid lipase A (LIPA). LIPA is essential for macrophage cholesterol metabolism. Immunohistological analyses of WIPF3 protein in AAA samples from three subjects revealed that WIPF3 was expressed in macrophages of atheromatous plaques. CONCLUSIONS: This study suggests that WIPF3 and LIPA, both of which are expressed in the macrophages are involved in pathological AAA. These results should be regarded as hypothesis-generating; thus, replication study is warranted. |
format | Online Article Text |
id | pubmed-5351826 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Science Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-53518262017-03-20 Association of non-synonymous variants in WIPF3 and LIPA genes with abdominal aortic aneurysm: an autopsy study Maeda, Yuko Sato, Noriko Naka-Mieno, Makiko Mori, Seijiro Arai, Tomio Tanaka, Masashi Muramatsu, Masaaki Sawabe, Motoji J Geriatr Cardiol Research Article BACKGROUND: Abdominal aortic aneurysm (AAA) is a multifactorial disease with strong genetic components. Various genetic loci have been associated with clinical AAA, but few studies have investigated pathological AAA, an intermediate phenotype of the disease. METHODS: We examined 2263 consecutive autopsies of older Japanese subjects from a study on geriatric diseases in Japanese individuals (The JG-SNP study). The presence of AAA was determined with a pathological diagnosis during autopsy. Single nucleotide variants (SNVs) associated with AAA were determined with an Illumina HumanExome Beadchip array. Logistic regression analyses were performed to determine genetic associations. Age, gender, and other risk factors of AAA were analyzed as covariates. RESULTS: 118 subjects with AAA and 2145 subjects without AAA were analyzed in a case-control setting. No variants reached significance after applying the Bonferroni correction (P < 2.05×10(−6)). The strongest associations were found with rs3750092 (p.E321G, OR: 0.36, 95% CI: 0.24–0.56, P = 6.09 × 10(−6)), a variant in the WAS/WASL interacting protein family 3 (WIPF3), and with rs1051338 (p.T16P, OR: 2.50, 95% CI: 1.70–3.66, P = 2.79 × 10(−6)) and rs2246942 (intronic, OR: 2.32, 95% CI: 1.58–3.41, P = 1.61 × 10(−5)), variants in the lysosomal acid lipase A (LIPA). LIPA is essential for macrophage cholesterol metabolism. Immunohistological analyses of WIPF3 protein in AAA samples from three subjects revealed that WIPF3 was expressed in macrophages of atheromatous plaques. CONCLUSIONS: This study suggests that WIPF3 and LIPA, both of which are expressed in the macrophages are involved in pathological AAA. These results should be regarded as hypothesis-generating; thus, replication study is warranted. Science Press 2016-12 /pmc/articles/PMC5351826/ /pubmed/28321238 http://dx.doi.org/10.11909/j.issn.1671-5411.2016.12.003 Text en Institute of Geriatric Cardiology http://creativecommons.org/licenses/by-nc-sa/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License, which allows readers to alter, transform, or build upon the article and then distribute the resulting work under the same or similar license to this one. The work must be attributed back to the original author and commercial use is not permitted without specific permission. |
spellingShingle | Research Article Maeda, Yuko Sato, Noriko Naka-Mieno, Makiko Mori, Seijiro Arai, Tomio Tanaka, Masashi Muramatsu, Masaaki Sawabe, Motoji Association of non-synonymous variants in WIPF3 and LIPA genes with abdominal aortic aneurysm: an autopsy study |
title | Association of non-synonymous variants in WIPF3 and LIPA genes with abdominal aortic aneurysm: an autopsy study |
title_full | Association of non-synonymous variants in WIPF3 and LIPA genes with abdominal aortic aneurysm: an autopsy study |
title_fullStr | Association of non-synonymous variants in WIPF3 and LIPA genes with abdominal aortic aneurysm: an autopsy study |
title_full_unstemmed | Association of non-synonymous variants in WIPF3 and LIPA genes with abdominal aortic aneurysm: an autopsy study |
title_short | Association of non-synonymous variants in WIPF3 and LIPA genes with abdominal aortic aneurysm: an autopsy study |
title_sort | association of non-synonymous variants in wipf3 and lipa genes with abdominal aortic aneurysm: an autopsy study |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5351826/ https://www.ncbi.nlm.nih.gov/pubmed/28321238 http://dx.doi.org/10.11909/j.issn.1671-5411.2016.12.003 |
work_keys_str_mv | AT maedayuko associationofnonsynonymousvariantsinwipf3andlipageneswithabdominalaorticaneurysmanautopsystudy AT satonoriko associationofnonsynonymousvariantsinwipf3andlipageneswithabdominalaorticaneurysmanautopsystudy AT nakamienomakiko associationofnonsynonymousvariantsinwipf3andlipageneswithabdominalaorticaneurysmanautopsystudy AT moriseijiro associationofnonsynonymousvariantsinwipf3andlipageneswithabdominalaorticaneurysmanautopsystudy AT araitomio associationofnonsynonymousvariantsinwipf3andlipageneswithabdominalaorticaneurysmanautopsystudy AT tanakamasashi associationofnonsynonymousvariantsinwipf3andlipageneswithabdominalaorticaneurysmanautopsystudy AT muramatsumasaaki associationofnonsynonymousvariantsinwipf3andlipageneswithabdominalaorticaneurysmanautopsystudy AT sawabemotoji associationofnonsynonymousvariantsinwipf3andlipageneswithabdominalaorticaneurysmanautopsystudy |