Cargando…
EQUIFAT: A novel scoring system for the semi-quantitative evaluation of regional adipose tissues in Equidae
Anatomically distinct adipose tissues represent variable risks to metabolic health in man and some other mammals. Quantitative-imaging of internal adipose depots is problematic in large animals and associations between regional adiposity and health are poorly understood. This study aimed to develop...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5351866/ https://www.ncbi.nlm.nih.gov/pubmed/28296956 http://dx.doi.org/10.1371/journal.pone.0173753 |
_version_ | 1782514840791678976 |
---|---|
author | Morrison, Philippa K. Harris, Patricia A. Maltin, Charlotte A. Grove-White, Dai Argo, Caroline McG. |
author_facet | Morrison, Philippa K. Harris, Patricia A. Maltin, Charlotte A. Grove-White, Dai Argo, Caroline McG. |
author_sort | Morrison, Philippa K. |
collection | PubMed |
description | Anatomically distinct adipose tissues represent variable risks to metabolic health in man and some other mammals. Quantitative-imaging of internal adipose depots is problematic in large animals and associations between regional adiposity and health are poorly understood. This study aimed to develop and test a semi-quantitative system (EQUIFAT) which could be applied to regional adipose tissues. Anatomically-defined, photographic images of adipose depots (omental, mesenteric, epicardial, rump) were collected from 38 animals immediately post-mortem. Images were ranked and depot-specific descriptors were developed (1 = no fat visible; 5 = excessive fat present). Nuchal-crest and ventro-abdominal-retroperitoneal adipose depot depths (cm) were transformed to categorical 5 point scores. The repeatability and reliability of EQUIFAT was independently tested by 24 observers. When half scores were permitted, inter-observer agreement was substantial (average κ(w): mesenteric, 0.79; omental, 0.79; rump 0.61) or moderate (average κ(w;) epicardial, 0.60). Intra-observer repeatability was tested by 8 observers on 2 occasions. Kappa analysis indicated perfect (omental and mesenteric) and substantial agreement (epicardial and rump) between attempts. A further 207 animals were evaluated ante-mortem (age, height, breed-type, gender, body condition score [BCS]) and again immediately post-mortem (EQUIFAT scores, carcass weight). Multivariable, random effect linear regression models were fitted (breed as random effect; BCS as outcome variable). Only height, carcass weight, omental and retroperitoneal EQUIFAT scores remained as explanatory variables in the final model. The EQUIFAT scores developed here demonstrate clear functional differences between regional adipose depots and future studies could be directed towards describing associations between adiposity and disease risk in surgical and post-mortem situations. |
format | Online Article Text |
id | pubmed-5351866 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-53518662017-04-06 EQUIFAT: A novel scoring system for the semi-quantitative evaluation of regional adipose tissues in Equidae Morrison, Philippa K. Harris, Patricia A. Maltin, Charlotte A. Grove-White, Dai Argo, Caroline McG. PLoS One Research Article Anatomically distinct adipose tissues represent variable risks to metabolic health in man and some other mammals. Quantitative-imaging of internal adipose depots is problematic in large animals and associations between regional adiposity and health are poorly understood. This study aimed to develop and test a semi-quantitative system (EQUIFAT) which could be applied to regional adipose tissues. Anatomically-defined, photographic images of adipose depots (omental, mesenteric, epicardial, rump) were collected from 38 animals immediately post-mortem. Images were ranked and depot-specific descriptors were developed (1 = no fat visible; 5 = excessive fat present). Nuchal-crest and ventro-abdominal-retroperitoneal adipose depot depths (cm) were transformed to categorical 5 point scores. The repeatability and reliability of EQUIFAT was independently tested by 24 observers. When half scores were permitted, inter-observer agreement was substantial (average κ(w): mesenteric, 0.79; omental, 0.79; rump 0.61) or moderate (average κ(w;) epicardial, 0.60). Intra-observer repeatability was tested by 8 observers on 2 occasions. Kappa analysis indicated perfect (omental and mesenteric) and substantial agreement (epicardial and rump) between attempts. A further 207 animals were evaluated ante-mortem (age, height, breed-type, gender, body condition score [BCS]) and again immediately post-mortem (EQUIFAT scores, carcass weight). Multivariable, random effect linear regression models were fitted (breed as random effect; BCS as outcome variable). Only height, carcass weight, omental and retroperitoneal EQUIFAT scores remained as explanatory variables in the final model. The EQUIFAT scores developed here demonstrate clear functional differences between regional adipose depots and future studies could be directed towards describing associations between adiposity and disease risk in surgical and post-mortem situations. Public Library of Science 2017-03-15 /pmc/articles/PMC5351866/ /pubmed/28296956 http://dx.doi.org/10.1371/journal.pone.0173753 Text en © 2017 Morrison et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Morrison, Philippa K. Harris, Patricia A. Maltin, Charlotte A. Grove-White, Dai Argo, Caroline McG. EQUIFAT: A novel scoring system for the semi-quantitative evaluation of regional adipose tissues in Equidae |
title | EQUIFAT: A novel scoring system for the semi-quantitative evaluation of regional adipose tissues in Equidae |
title_full | EQUIFAT: A novel scoring system for the semi-quantitative evaluation of regional adipose tissues in Equidae |
title_fullStr | EQUIFAT: A novel scoring system for the semi-quantitative evaluation of regional adipose tissues in Equidae |
title_full_unstemmed | EQUIFAT: A novel scoring system for the semi-quantitative evaluation of regional adipose tissues in Equidae |
title_short | EQUIFAT: A novel scoring system for the semi-quantitative evaluation of regional adipose tissues in Equidae |
title_sort | equifat: a novel scoring system for the semi-quantitative evaluation of regional adipose tissues in equidae |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5351866/ https://www.ncbi.nlm.nih.gov/pubmed/28296956 http://dx.doi.org/10.1371/journal.pone.0173753 |
work_keys_str_mv | AT morrisonphilippak equifatanovelscoringsystemforthesemiquantitativeevaluationofregionaladiposetissuesinequidae AT harrispatriciaa equifatanovelscoringsystemforthesemiquantitativeevaluationofregionaladiposetissuesinequidae AT maltincharlottea equifatanovelscoringsystemforthesemiquantitativeevaluationofregionaladiposetissuesinequidae AT grovewhitedai equifatanovelscoringsystemforthesemiquantitativeevaluationofregionaladiposetissuesinequidae AT argocarolinemcg equifatanovelscoringsystemforthesemiquantitativeevaluationofregionaladiposetissuesinequidae |