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Independently founded populations of Sclerotinia sclerotiorum from a tropical and a temperate region have similar genetic structure

Sclerotinia sclerotiorum populations from tropical agricultural zones have been suggested to be more variable compared to those from temperate zones. However, no data were available comparing populations from both zones using the same set of markers. In this study, we compared S. sclerotiorum popula...

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Autores principales: Lehner, Miller S., de Paula Júnior, Trazilbo J., Del Ponte, Emerson M., Mizubuti, Eduardo S. G., Pethybridge, Sarah J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5352009/
https://www.ncbi.nlm.nih.gov/pubmed/28296968
http://dx.doi.org/10.1371/journal.pone.0173915
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author Lehner, Miller S.
de Paula Júnior, Trazilbo J.
Del Ponte, Emerson M.
Mizubuti, Eduardo S. G.
Pethybridge, Sarah J.
author_facet Lehner, Miller S.
de Paula Júnior, Trazilbo J.
Del Ponte, Emerson M.
Mizubuti, Eduardo S. G.
Pethybridge, Sarah J.
author_sort Lehner, Miller S.
collection PubMed
description Sclerotinia sclerotiorum populations from tropical agricultural zones have been suggested to be more variable compared to those from temperate zones. However, no data were available comparing populations from both zones using the same set of markers. In this study, we compared S. sclerotiorum populations from the United States of America (USA, temperate) and southeast Brazil (tropical) using the frequency of mycelial compatibility groups (MCGs) and 13 microsatellite (SSR) markers. Populations were sourced from diseased plants within leguminous crops in New York, USA (NY; n = 78 isolates), and Minas Gerais State, Brazil (MG; n = 109). Twenty MCGs were identified in NY and 14 were previously reported in MG. The effective number of genotypes based on Hill’s number of order 0, which corresponded to the number of multilocus genotypes (MLGs) were 22 (95% CI = 15.6–28.4) and 24 (95% CI = 18.9–29.1) in NY and MG, respectively. Clonal fractions of MLGs were 71.8% (NY) and 78.0% (MG). The effective number of genotypes based on Hill’s number of orders 1 and 2 in NY were 8.9 (95% CI = 5.2–12.6) and 4.4 (95% CI = 2.6–6.1), respectively. For MG these indices were 11.4 (95% CI = 8.7–14.1) and 7.1 (95% CI = 5.1–9.0), respectively. There were no significant differences of allelic richness, private allelic richness, gene diversity, effective number of alleles and genotype evenness between the NY and MG populations. The populations were differentiated, with 29% of total variance attributed to differences between them and G''(ST) and Jost’s D indices higher than 0.50. Cluster analysis revealed dissimilarity higher than 80% among most MLGs from both populations. Different alleles segregated in the populations but both had similar levels of genotypic variability.
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spelling pubmed-53520092017-04-06 Independently founded populations of Sclerotinia sclerotiorum from a tropical and a temperate region have similar genetic structure Lehner, Miller S. de Paula Júnior, Trazilbo J. Del Ponte, Emerson M. Mizubuti, Eduardo S. G. Pethybridge, Sarah J. PLoS One Research Article Sclerotinia sclerotiorum populations from tropical agricultural zones have been suggested to be more variable compared to those from temperate zones. However, no data were available comparing populations from both zones using the same set of markers. In this study, we compared S. sclerotiorum populations from the United States of America (USA, temperate) and southeast Brazil (tropical) using the frequency of mycelial compatibility groups (MCGs) and 13 microsatellite (SSR) markers. Populations were sourced from diseased plants within leguminous crops in New York, USA (NY; n = 78 isolates), and Minas Gerais State, Brazil (MG; n = 109). Twenty MCGs were identified in NY and 14 were previously reported in MG. The effective number of genotypes based on Hill’s number of order 0, which corresponded to the number of multilocus genotypes (MLGs) were 22 (95% CI = 15.6–28.4) and 24 (95% CI = 18.9–29.1) in NY and MG, respectively. Clonal fractions of MLGs were 71.8% (NY) and 78.0% (MG). The effective number of genotypes based on Hill’s number of orders 1 and 2 in NY were 8.9 (95% CI = 5.2–12.6) and 4.4 (95% CI = 2.6–6.1), respectively. For MG these indices were 11.4 (95% CI = 8.7–14.1) and 7.1 (95% CI = 5.1–9.0), respectively. There were no significant differences of allelic richness, private allelic richness, gene diversity, effective number of alleles and genotype evenness between the NY and MG populations. The populations were differentiated, with 29% of total variance attributed to differences between them and G''(ST) and Jost’s D indices higher than 0.50. Cluster analysis revealed dissimilarity higher than 80% among most MLGs from both populations. Different alleles segregated in the populations but both had similar levels of genotypic variability. Public Library of Science 2017-03-15 /pmc/articles/PMC5352009/ /pubmed/28296968 http://dx.doi.org/10.1371/journal.pone.0173915 Text en © 2017 Lehner et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Lehner, Miller S.
de Paula Júnior, Trazilbo J.
Del Ponte, Emerson M.
Mizubuti, Eduardo S. G.
Pethybridge, Sarah J.
Independently founded populations of Sclerotinia sclerotiorum from a tropical and a temperate region have similar genetic structure
title Independently founded populations of Sclerotinia sclerotiorum from a tropical and a temperate region have similar genetic structure
title_full Independently founded populations of Sclerotinia sclerotiorum from a tropical and a temperate region have similar genetic structure
title_fullStr Independently founded populations of Sclerotinia sclerotiorum from a tropical and a temperate region have similar genetic structure
title_full_unstemmed Independently founded populations of Sclerotinia sclerotiorum from a tropical and a temperate region have similar genetic structure
title_short Independently founded populations of Sclerotinia sclerotiorum from a tropical and a temperate region have similar genetic structure
title_sort independently founded populations of sclerotinia sclerotiorum from a tropical and a temperate region have similar genetic structure
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5352009/
https://www.ncbi.nlm.nih.gov/pubmed/28296968
http://dx.doi.org/10.1371/journal.pone.0173915
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