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Feasibility of assessing bone matrix and mineral properties in vivo by combined solid-state (1)H and (31)P MRI

PURPOSE: To develop and evaluate an integrated imaging protocol for bone water and phosphorus quantification in vivo by solid-state (1)H and (31)P MRI. MATERIALS AND METHODS: All studies were HIPAA-compliant and were performed with institutional review board approval and written informed consent. Pr...

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Autores principales: Zhao, Xia, Song, Hee Kwon, Seifert, Alan C., Li, Cheng, Wehrli, Felix W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5352014/
https://www.ncbi.nlm.nih.gov/pubmed/28296979
http://dx.doi.org/10.1371/journal.pone.0173995
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author Zhao, Xia
Song, Hee Kwon
Seifert, Alan C.
Li, Cheng
Wehrli, Felix W.
author_facet Zhao, Xia
Song, Hee Kwon
Seifert, Alan C.
Li, Cheng
Wehrli, Felix W.
author_sort Zhao, Xia
collection PubMed
description PURPOSE: To develop and evaluate an integrated imaging protocol for bone water and phosphorus quantification in vivo by solid-state (1)H and (31)P MRI. MATERIALS AND METHODS: All studies were HIPAA-compliant and were performed with institutional review board approval and written informed consent. Proton ((1)H) ultra-short echo-time (UTE) and phosphorus ((31)P) zero echo-time (ZTE) sequences were designed and implemented on a 3 T clinical MR scanner to quantify bone water and mineral in vivo. The left tibia of ten healthy subjects (including both genders, 49±15 y/o) was examined with a custom-built (1)H/(31)P dual-frequency extremity RF coil. Total bone water (TW), water bound to the collagen matrix (BW) and bone (31)P were quantified from MR images with respect to reference samples of known (1)H or (31)P concentration, and pore water (PW) was subsequently determined from TW and BW. Porosity index (PI) was calculated as the ratio between UTE images acquired at two echo times. MRI parameters were compared with bone density measures obtained by high-resolution peripheral quantitative CT (HR-pQCT). RESULTS: The total scan time for the bone water and (31)P quantification protocol was about 50 minutes. Average TW, BW, PW and (31)P concentrations were 13.99±1.26, 10.39±0.80, 3.34±1.41 mol/L and 7.06±1.53 mol/L for the studied cohort, respectively, in good agreement with previous results conducted ex vivo. Average intra-subject coefficients of variation were 3.47%, 2.60% and 7.50% for TW, BW and PW and 5.60% for (31)P. Negative correlations were observed between PW and vBMD (p<0.05) as well as between PI and (31)P (p<0.05), while bone mineral content (BMC) estimated from (31)P MRI and HR-pQCT were strongly positively correlated (p<0.0001). CONCLUSION: This work demonstrates the feasibility of quantifying bone water and mineral phosphorus in human subjects in a single MRI session with a clinically practical imaging protocol.
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spelling pubmed-53520142017-04-06 Feasibility of assessing bone matrix and mineral properties in vivo by combined solid-state (1)H and (31)P MRI Zhao, Xia Song, Hee Kwon Seifert, Alan C. Li, Cheng Wehrli, Felix W. PLoS One Research Article PURPOSE: To develop and evaluate an integrated imaging protocol for bone water and phosphorus quantification in vivo by solid-state (1)H and (31)P MRI. MATERIALS AND METHODS: All studies were HIPAA-compliant and were performed with institutional review board approval and written informed consent. Proton ((1)H) ultra-short echo-time (UTE) and phosphorus ((31)P) zero echo-time (ZTE) sequences were designed and implemented on a 3 T clinical MR scanner to quantify bone water and mineral in vivo. The left tibia of ten healthy subjects (including both genders, 49±15 y/o) was examined with a custom-built (1)H/(31)P dual-frequency extremity RF coil. Total bone water (TW), water bound to the collagen matrix (BW) and bone (31)P were quantified from MR images with respect to reference samples of known (1)H or (31)P concentration, and pore water (PW) was subsequently determined from TW and BW. Porosity index (PI) was calculated as the ratio between UTE images acquired at two echo times. MRI parameters were compared with bone density measures obtained by high-resolution peripheral quantitative CT (HR-pQCT). RESULTS: The total scan time for the bone water and (31)P quantification protocol was about 50 minutes. Average TW, BW, PW and (31)P concentrations were 13.99±1.26, 10.39±0.80, 3.34±1.41 mol/L and 7.06±1.53 mol/L for the studied cohort, respectively, in good agreement with previous results conducted ex vivo. Average intra-subject coefficients of variation were 3.47%, 2.60% and 7.50% for TW, BW and PW and 5.60% for (31)P. Negative correlations were observed between PW and vBMD (p<0.05) as well as between PI and (31)P (p<0.05), while bone mineral content (BMC) estimated from (31)P MRI and HR-pQCT were strongly positively correlated (p<0.0001). CONCLUSION: This work demonstrates the feasibility of quantifying bone water and mineral phosphorus in human subjects in a single MRI session with a clinically practical imaging protocol. Public Library of Science 2017-03-15 /pmc/articles/PMC5352014/ /pubmed/28296979 http://dx.doi.org/10.1371/journal.pone.0173995 Text en © 2017 Zhao et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Zhao, Xia
Song, Hee Kwon
Seifert, Alan C.
Li, Cheng
Wehrli, Felix W.
Feasibility of assessing bone matrix and mineral properties in vivo by combined solid-state (1)H and (31)P MRI
title Feasibility of assessing bone matrix and mineral properties in vivo by combined solid-state (1)H and (31)P MRI
title_full Feasibility of assessing bone matrix and mineral properties in vivo by combined solid-state (1)H and (31)P MRI
title_fullStr Feasibility of assessing bone matrix and mineral properties in vivo by combined solid-state (1)H and (31)P MRI
title_full_unstemmed Feasibility of assessing bone matrix and mineral properties in vivo by combined solid-state (1)H and (31)P MRI
title_short Feasibility of assessing bone matrix and mineral properties in vivo by combined solid-state (1)H and (31)P MRI
title_sort feasibility of assessing bone matrix and mineral properties in vivo by combined solid-state (1)h and (31)p mri
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5352014/
https://www.ncbi.nlm.nih.gov/pubmed/28296979
http://dx.doi.org/10.1371/journal.pone.0173995
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