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Exosome secretion affects social motility in Trypanosoma brucei

Extracellular vesicles (EV) secreted by pathogens function in a variety of biological processes. Here, we demonstrate that in the protozoan parasite Trypanosoma brucei, exosome secretion is induced by stress that affects trans-splicing. Following perturbations in biogenesis of spliced leader RNA, wh...

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Autores principales: Eliaz, Dror, Kannan, Sriram, Shaked, Hadassa, Arvatz, Gil, Tkacz, Itai Dov, Binder, Lior, Waldman Ben-Asher, Hiba, Okalang, Uthman, Chikne, Vaibhav, Cohen-Chalamish, Smadar, Michaeli, Shulamit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5352147/
https://www.ncbi.nlm.nih.gov/pubmed/28257521
http://dx.doi.org/10.1371/journal.ppat.1006245
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author Eliaz, Dror
Kannan, Sriram
Shaked, Hadassa
Arvatz, Gil
Tkacz, Itai Dov
Binder, Lior
Waldman Ben-Asher, Hiba
Okalang, Uthman
Chikne, Vaibhav
Cohen-Chalamish, Smadar
Michaeli, Shulamit
author_facet Eliaz, Dror
Kannan, Sriram
Shaked, Hadassa
Arvatz, Gil
Tkacz, Itai Dov
Binder, Lior
Waldman Ben-Asher, Hiba
Okalang, Uthman
Chikne, Vaibhav
Cohen-Chalamish, Smadar
Michaeli, Shulamit
author_sort Eliaz, Dror
collection PubMed
description Extracellular vesicles (EV) secreted by pathogens function in a variety of biological processes. Here, we demonstrate that in the protozoan parasite Trypanosoma brucei, exosome secretion is induced by stress that affects trans-splicing. Following perturbations in biogenesis of spliced leader RNA, which donates its spliced leader (SL) exon to all mRNAs, or after heat-shock, the SL RNA is exported to the cytoplasm and forms distinct granules, which are then secreted by exosomes. The exosomes are formed in multivesicular bodies (MVB) utilizing the endosomal sorting complexes required for transport (ESCRT), through a mechanism similar to microRNA secretion in mammalian cells. Silencing of the ESCRT factor, Vps36, compromised exosome secretion but not the secretion of vesicles derived from nanotubes. The exosomes enter recipient trypanosome cells. Time-lapse microscopy demonstrated that cells secreting exosomes or purified intact exosomes affect social motility (SoMo). This study demonstrates that exosomes are delivered to trypanosome cells and can change their migration. Exosomes are used to transmit stress signals for communication between parasites.
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spelling pubmed-53521472017-04-06 Exosome secretion affects social motility in Trypanosoma brucei Eliaz, Dror Kannan, Sriram Shaked, Hadassa Arvatz, Gil Tkacz, Itai Dov Binder, Lior Waldman Ben-Asher, Hiba Okalang, Uthman Chikne, Vaibhav Cohen-Chalamish, Smadar Michaeli, Shulamit PLoS Pathog Research Article Extracellular vesicles (EV) secreted by pathogens function in a variety of biological processes. Here, we demonstrate that in the protozoan parasite Trypanosoma brucei, exosome secretion is induced by stress that affects trans-splicing. Following perturbations in biogenesis of spliced leader RNA, which donates its spliced leader (SL) exon to all mRNAs, or after heat-shock, the SL RNA is exported to the cytoplasm and forms distinct granules, which are then secreted by exosomes. The exosomes are formed in multivesicular bodies (MVB) utilizing the endosomal sorting complexes required for transport (ESCRT), through a mechanism similar to microRNA secretion in mammalian cells. Silencing of the ESCRT factor, Vps36, compromised exosome secretion but not the secretion of vesicles derived from nanotubes. The exosomes enter recipient trypanosome cells. Time-lapse microscopy demonstrated that cells secreting exosomes or purified intact exosomes affect social motility (SoMo). This study demonstrates that exosomes are delivered to trypanosome cells and can change their migration. Exosomes are used to transmit stress signals for communication between parasites. Public Library of Science 2017-03-03 /pmc/articles/PMC5352147/ /pubmed/28257521 http://dx.doi.org/10.1371/journal.ppat.1006245 Text en © 2017 Eliaz et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Eliaz, Dror
Kannan, Sriram
Shaked, Hadassa
Arvatz, Gil
Tkacz, Itai Dov
Binder, Lior
Waldman Ben-Asher, Hiba
Okalang, Uthman
Chikne, Vaibhav
Cohen-Chalamish, Smadar
Michaeli, Shulamit
Exosome secretion affects social motility in Trypanosoma brucei
title Exosome secretion affects social motility in Trypanosoma brucei
title_full Exosome secretion affects social motility in Trypanosoma brucei
title_fullStr Exosome secretion affects social motility in Trypanosoma brucei
title_full_unstemmed Exosome secretion affects social motility in Trypanosoma brucei
title_short Exosome secretion affects social motility in Trypanosoma brucei
title_sort exosome secretion affects social motility in trypanosoma brucei
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5352147/
https://www.ncbi.nlm.nih.gov/pubmed/28257521
http://dx.doi.org/10.1371/journal.ppat.1006245
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