Antifungal peptidic compound from the deep-sea bacterium Aneurinibacillus sp. YR247

Aneurinibacillus: sp. YR247 was newly isolated from the deep-sea sediment inside the Calyptogena community at a depth of 1171 m in Sagami Bay. The strain exhibited antifungal activity against the filamentous fungus Aspergillus brasiliensis NBRC9455. A crude extract prepared from the YR247 cells by e...

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Autores principales: Kurata, Atsushi, Yamaura, Yuto, Tanaka, Takumi, Kato, Chiaki, Nakasone, Kaoru, Kishimoto, Noriaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2017
Materias:
Original Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5352791/
https://www.ncbi.nlm.nih.gov/pubmed/28299556
http://dx.doi.org/10.1007/s11274-017-2239-0
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author Kurata, Atsushi
Yamaura, Yuto
Tanaka, Takumi
Kato, Chiaki
Nakasone, Kaoru
Kishimoto, Noriaki
author_facet Kurata, Atsushi
Yamaura, Yuto
Tanaka, Takumi
Kato, Chiaki
Nakasone, Kaoru
Kishimoto, Noriaki
author_sort Kurata, Atsushi
collection PubMed
description Aneurinibacillus: sp. YR247 was newly isolated from the deep-sea sediment inside the Calyptogena community at a depth of 1171 m in Sagami Bay. The strain exhibited antifungal activity against the filamentous fungus Aspergillus brasiliensis NBRC9455. A crude extract prepared from the YR247 cells by ethanol extraction exhibited broad antimicrobial activities. The antifungal compound is stable at 4–70 °C and pH 2.0–12.0. After treatment with proteinase K, the antifungal activity was not detected, indicating that the antifungal compound of strain YR247 is a peptidic compound. Electrospray ionization mass spectrometry of the purified antifungal compound indicated that the peptidic compound has an average molecular weight of 1167.9. The molecular weight of the antifungal compound from strain YR247 is different from those of antimicrobial peptides produced by the related Aneurinibacillus and Bacillus bacteria. The antifungal peptidic compound from the deep-sea bacterium Aneurinibacillus sp. YR247 may be useful as a biocontrol agent.
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spelling pubmed-53527912017-03-28 Antifungal peptidic compound from the deep-sea bacterium Aneurinibacillus sp. YR247 Kurata, Atsushi Yamaura, Yuto Tanaka, Takumi Kato, Chiaki Nakasone, Kaoru Kishimoto, Noriaki World J Microbiol Biotechnol Original Paper Aneurinibacillus: sp. YR247 was newly isolated from the deep-sea sediment inside the Calyptogena community at a depth of 1171 m in Sagami Bay. The strain exhibited antifungal activity against the filamentous fungus Aspergillus brasiliensis NBRC9455. A crude extract prepared from the YR247 cells by ethanol extraction exhibited broad antimicrobial activities. The antifungal compound is stable at 4–70 °C and pH 2.0–12.0. After treatment with proteinase K, the antifungal activity was not detected, indicating that the antifungal compound of strain YR247 is a peptidic compound. Electrospray ionization mass spectrometry of the purified antifungal compound indicated that the peptidic compound has an average molecular weight of 1167.9. The molecular weight of the antifungal compound from strain YR247 is different from those of antimicrobial peptides produced by the related Aneurinibacillus and Bacillus bacteria. The antifungal peptidic compound from the deep-sea bacterium Aneurinibacillus sp. YR247 may be useful as a biocontrol agent. Springer Netherlands 2017-03-15 2017 /pmc/articles/PMC5352791/ /pubmed/28299556 http://dx.doi.org/10.1007/s11274-017-2239-0 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Paper
Kurata, Atsushi
Yamaura, Yuto
Tanaka, Takumi
Kato, Chiaki
Nakasone, Kaoru
Kishimoto, Noriaki
Antifungal peptidic compound from the deep-sea bacterium Aneurinibacillus sp. YR247
title Antifungal peptidic compound from the deep-sea bacterium Aneurinibacillus sp. YR247
title_full Antifungal peptidic compound from the deep-sea bacterium Aneurinibacillus sp. YR247
title_fullStr Antifungal peptidic compound from the deep-sea bacterium Aneurinibacillus sp. YR247
title_full_unstemmed Antifungal peptidic compound from the deep-sea bacterium Aneurinibacillus sp. YR247
title_short Antifungal peptidic compound from the deep-sea bacterium Aneurinibacillus sp. YR247
title_sort antifungal peptidic compound from the deep-sea bacterium aneurinibacillus sp. yr247
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5352791/
https://www.ncbi.nlm.nih.gov/pubmed/28299556
http://dx.doi.org/10.1007/s11274-017-2239-0
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