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BAG5 Interacts with DJ-1 and Inhibits the Neuroprotective Effects of DJ-1 to Combat Mitochondrial Oxidative Damage
Loss-of-function mutations in gene encoding DJ-1 contribute to the pathogenesis of autosomal recessive early-onset familial forms of Parkinson's disease (PD). DJ-1 is a multifunctional protein and plays a protective role against oxidative stress-induced mitochondrial damage and cell death, but...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5352890/ https://www.ncbi.nlm.nih.gov/pubmed/28348719 http://dx.doi.org/10.1155/2017/5094934 |
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author | Qin, Li-xia Tan, Jie-qiong Zhang, Hai-nan Rizwana, Kousar Lu, Jia-hong Tang, Jian-guang Jiang, Bo Shen, Xiang-min Guo, Ji-feng Tang, Bei-sha Tan, Li-ming Wang, Chun-yu |
author_facet | Qin, Li-xia Tan, Jie-qiong Zhang, Hai-nan Rizwana, Kousar Lu, Jia-hong Tang, Jian-guang Jiang, Bo Shen, Xiang-min Guo, Ji-feng Tang, Bei-sha Tan, Li-ming Wang, Chun-yu |
author_sort | Qin, Li-xia |
collection | PubMed |
description | Loss-of-function mutations in gene encoding DJ-1 contribute to the pathogenesis of autosomal recessive early-onset familial forms of Parkinson's disease (PD). DJ-1 is a multifunctional protein and plays a protective role against oxidative stress-induced mitochondrial damage and cell death, but the exact mechanism underlying this is not yet clearly understood. Here, using coimmunoprecipitation (Co-IP) and immunofluorescence methods, we prove that Bcl-2-associated athanogene 5 (BAG5), a BAG family member, interacts with DJ-1 in mammalian cells. Moreover, we show that BAG5 could decrease stability of DJ-1 and weaken its role in mitochondrial protection probably by influencing dimerization in stress condition. Our study reveals the relationship of BAG5 and DJ-1 suggesting a potential role for BAG5 in the pathogenesis of PD through its functional interactions with DJ-1. |
format | Online Article Text |
id | pubmed-5352890 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-53528902017-03-27 BAG5 Interacts with DJ-1 and Inhibits the Neuroprotective Effects of DJ-1 to Combat Mitochondrial Oxidative Damage Qin, Li-xia Tan, Jie-qiong Zhang, Hai-nan Rizwana, Kousar Lu, Jia-hong Tang, Jian-guang Jiang, Bo Shen, Xiang-min Guo, Ji-feng Tang, Bei-sha Tan, Li-ming Wang, Chun-yu Oxid Med Cell Longev Research Article Loss-of-function mutations in gene encoding DJ-1 contribute to the pathogenesis of autosomal recessive early-onset familial forms of Parkinson's disease (PD). DJ-1 is a multifunctional protein and plays a protective role against oxidative stress-induced mitochondrial damage and cell death, but the exact mechanism underlying this is not yet clearly understood. Here, using coimmunoprecipitation (Co-IP) and immunofluorescence methods, we prove that Bcl-2-associated athanogene 5 (BAG5), a BAG family member, interacts with DJ-1 in mammalian cells. Moreover, we show that BAG5 could decrease stability of DJ-1 and weaken its role in mitochondrial protection probably by influencing dimerization in stress condition. Our study reveals the relationship of BAG5 and DJ-1 suggesting a potential role for BAG5 in the pathogenesis of PD through its functional interactions with DJ-1. Hindawi 2017 2017-03-02 /pmc/articles/PMC5352890/ /pubmed/28348719 http://dx.doi.org/10.1155/2017/5094934 Text en Copyright © 2017 Li-xia Qin et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Qin, Li-xia Tan, Jie-qiong Zhang, Hai-nan Rizwana, Kousar Lu, Jia-hong Tang, Jian-guang Jiang, Bo Shen, Xiang-min Guo, Ji-feng Tang, Bei-sha Tan, Li-ming Wang, Chun-yu BAG5 Interacts with DJ-1 and Inhibits the Neuroprotective Effects of DJ-1 to Combat Mitochondrial Oxidative Damage |
title | BAG5 Interacts with DJ-1 and Inhibits the Neuroprotective Effects of DJ-1 to Combat Mitochondrial Oxidative Damage |
title_full | BAG5 Interacts with DJ-1 and Inhibits the Neuroprotective Effects of DJ-1 to Combat Mitochondrial Oxidative Damage |
title_fullStr | BAG5 Interacts with DJ-1 and Inhibits the Neuroprotective Effects of DJ-1 to Combat Mitochondrial Oxidative Damage |
title_full_unstemmed | BAG5 Interacts with DJ-1 and Inhibits the Neuroprotective Effects of DJ-1 to Combat Mitochondrial Oxidative Damage |
title_short | BAG5 Interacts with DJ-1 and Inhibits the Neuroprotective Effects of DJ-1 to Combat Mitochondrial Oxidative Damage |
title_sort | bag5 interacts with dj-1 and inhibits the neuroprotective effects of dj-1 to combat mitochondrial oxidative damage |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5352890/ https://www.ncbi.nlm.nih.gov/pubmed/28348719 http://dx.doi.org/10.1155/2017/5094934 |
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