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BAG5 Interacts with DJ-1 and Inhibits the Neuroprotective Effects of DJ-1 to Combat Mitochondrial Oxidative Damage

Loss-of-function mutations in gene encoding DJ-1 contribute to the pathogenesis of autosomal recessive early-onset familial forms of Parkinson's disease (PD). DJ-1 is a multifunctional protein and plays a protective role against oxidative stress-induced mitochondrial damage and cell death, but...

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Autores principales: Qin, Li-xia, Tan, Jie-qiong, Zhang, Hai-nan, Rizwana, Kousar, Lu, Jia-hong, Tang, Jian-guang, Jiang, Bo, Shen, Xiang-min, Guo, Ji-feng, Tang, Bei-sha, Tan, Li-ming, Wang, Chun-yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5352890/
https://www.ncbi.nlm.nih.gov/pubmed/28348719
http://dx.doi.org/10.1155/2017/5094934
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author Qin, Li-xia
Tan, Jie-qiong
Zhang, Hai-nan
Rizwana, Kousar
Lu, Jia-hong
Tang, Jian-guang
Jiang, Bo
Shen, Xiang-min
Guo, Ji-feng
Tang, Bei-sha
Tan, Li-ming
Wang, Chun-yu
author_facet Qin, Li-xia
Tan, Jie-qiong
Zhang, Hai-nan
Rizwana, Kousar
Lu, Jia-hong
Tang, Jian-guang
Jiang, Bo
Shen, Xiang-min
Guo, Ji-feng
Tang, Bei-sha
Tan, Li-ming
Wang, Chun-yu
author_sort Qin, Li-xia
collection PubMed
description Loss-of-function mutations in gene encoding DJ-1 contribute to the pathogenesis of autosomal recessive early-onset familial forms of Parkinson's disease (PD). DJ-1 is a multifunctional protein and plays a protective role against oxidative stress-induced mitochondrial damage and cell death, but the exact mechanism underlying this is not yet clearly understood. Here, using coimmunoprecipitation (Co-IP) and immunofluorescence methods, we prove that Bcl-2-associated athanogene 5 (BAG5), a BAG family member, interacts with DJ-1 in mammalian cells. Moreover, we show that BAG5 could decrease stability of DJ-1 and weaken its role in mitochondrial protection probably by influencing dimerization in stress condition. Our study reveals the relationship of BAG5 and DJ-1 suggesting a potential role for BAG5 in the pathogenesis of PD through its functional interactions with DJ-1.
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spelling pubmed-53528902017-03-27 BAG5 Interacts with DJ-1 and Inhibits the Neuroprotective Effects of DJ-1 to Combat Mitochondrial Oxidative Damage Qin, Li-xia Tan, Jie-qiong Zhang, Hai-nan Rizwana, Kousar Lu, Jia-hong Tang, Jian-guang Jiang, Bo Shen, Xiang-min Guo, Ji-feng Tang, Bei-sha Tan, Li-ming Wang, Chun-yu Oxid Med Cell Longev Research Article Loss-of-function mutations in gene encoding DJ-1 contribute to the pathogenesis of autosomal recessive early-onset familial forms of Parkinson's disease (PD). DJ-1 is a multifunctional protein and plays a protective role against oxidative stress-induced mitochondrial damage and cell death, but the exact mechanism underlying this is not yet clearly understood. Here, using coimmunoprecipitation (Co-IP) and immunofluorescence methods, we prove that Bcl-2-associated athanogene 5 (BAG5), a BAG family member, interacts with DJ-1 in mammalian cells. Moreover, we show that BAG5 could decrease stability of DJ-1 and weaken its role in mitochondrial protection probably by influencing dimerization in stress condition. Our study reveals the relationship of BAG5 and DJ-1 suggesting a potential role for BAG5 in the pathogenesis of PD through its functional interactions with DJ-1. Hindawi 2017 2017-03-02 /pmc/articles/PMC5352890/ /pubmed/28348719 http://dx.doi.org/10.1155/2017/5094934 Text en Copyright © 2017 Li-xia Qin et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Qin, Li-xia
Tan, Jie-qiong
Zhang, Hai-nan
Rizwana, Kousar
Lu, Jia-hong
Tang, Jian-guang
Jiang, Bo
Shen, Xiang-min
Guo, Ji-feng
Tang, Bei-sha
Tan, Li-ming
Wang, Chun-yu
BAG5 Interacts with DJ-1 and Inhibits the Neuroprotective Effects of DJ-1 to Combat Mitochondrial Oxidative Damage
title BAG5 Interacts with DJ-1 and Inhibits the Neuroprotective Effects of DJ-1 to Combat Mitochondrial Oxidative Damage
title_full BAG5 Interacts with DJ-1 and Inhibits the Neuroprotective Effects of DJ-1 to Combat Mitochondrial Oxidative Damage
title_fullStr BAG5 Interacts with DJ-1 and Inhibits the Neuroprotective Effects of DJ-1 to Combat Mitochondrial Oxidative Damage
title_full_unstemmed BAG5 Interacts with DJ-1 and Inhibits the Neuroprotective Effects of DJ-1 to Combat Mitochondrial Oxidative Damage
title_short BAG5 Interacts with DJ-1 and Inhibits the Neuroprotective Effects of DJ-1 to Combat Mitochondrial Oxidative Damage
title_sort bag5 interacts with dj-1 and inhibits the neuroprotective effects of dj-1 to combat mitochondrial oxidative damage
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5352890/
https://www.ncbi.nlm.nih.gov/pubmed/28348719
http://dx.doi.org/10.1155/2017/5094934
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