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Double-flow focused liquid injector for efficient serial femtosecond crystallography
Serial femtosecond crystallography requires reliable and efficient delivery of fresh crystals across the beam of an X-ray free-electron laser over the course of an experiment. We introduce a double-flow focusing nozzle to meet this challenge, with significantly reduced sample consumption, while impr...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group
2017
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5353652/ https://www.ncbi.nlm.nih.gov/pubmed/28300169 http://dx.doi.org/10.1038/srep44628 |
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| author | Oberthuer, Dominik Knoška, Juraj Wiedorn, Max O. Beyerlein, Kenneth R. Bushnell, David A. Kovaleva, Elena G. Heymann, Michael Gumprecht, Lars Kirian, Richard A. Barty, Anton Mariani, Valerio Tolstikova, Aleksandra Adriano, Luigi Awel, Salah Barthelmess, Miriam Dörner, Katerina Xavier, P. Lourdu Yefanov, Oleksandr James, Daniel R. Nelson, Garrett Wang, Dingjie Calvey, George Chen, Yujie Schmidt, Andrea Szczepek, Michael Frielingsdorf, Stefan Lenz, Oliver Snell, Edward Robinson, Philip J. Šarler, Božidar Belšak, Grega Maček, Marjan Wilde, Fabian Aquila, Andrew Boutet, Sébastien Liang, Mengning Hunter, Mark S. Scheerer, Patrick Lipscomb, John D. Weierstall, Uwe Kornberg, Roger D. Spence, John C. H. Pollack, Lois Chapman, Henry N. Bajt, Saša |
| author_facet | Oberthuer, Dominik Knoška, Juraj Wiedorn, Max O. Beyerlein, Kenneth R. Bushnell, David A. Kovaleva, Elena G. Heymann, Michael Gumprecht, Lars Kirian, Richard A. Barty, Anton Mariani, Valerio Tolstikova, Aleksandra Adriano, Luigi Awel, Salah Barthelmess, Miriam Dörner, Katerina Xavier, P. Lourdu Yefanov, Oleksandr James, Daniel R. Nelson, Garrett Wang, Dingjie Calvey, George Chen, Yujie Schmidt, Andrea Szczepek, Michael Frielingsdorf, Stefan Lenz, Oliver Snell, Edward Robinson, Philip J. Šarler, Božidar Belšak, Grega Maček, Marjan Wilde, Fabian Aquila, Andrew Boutet, Sébastien Liang, Mengning Hunter, Mark S. Scheerer, Patrick Lipscomb, John D. Weierstall, Uwe Kornberg, Roger D. Spence, John C. H. Pollack, Lois Chapman, Henry N. Bajt, Saša |
| author_sort | Oberthuer, Dominik |
| collection | PubMed |
| description | Serial femtosecond crystallography requires reliable and efficient delivery of fresh crystals across the beam of an X-ray free-electron laser over the course of an experiment. We introduce a double-flow focusing nozzle to meet this challenge, with significantly reduced sample consumption, while improving jet stability over previous generations of nozzles. We demonstrate its use to determine the first room-temperature structure of RNA polymerase II at high resolution, revealing new structural details. Moreover, the double flow-focusing nozzles were successfully tested with three other protein samples and the first room temperature structure of an extradiol ring-cleaving dioxygenase was solved by utilizing the improved operation and characteristics of these devices. |
| format | Online Article Text |
| id | pubmed-5353652 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2017 |
| publisher | Nature Publishing Group |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-53536522017-03-20 Double-flow focused liquid injector for efficient serial femtosecond crystallography Oberthuer, Dominik Knoška, Juraj Wiedorn, Max O. Beyerlein, Kenneth R. Bushnell, David A. Kovaleva, Elena G. Heymann, Michael Gumprecht, Lars Kirian, Richard A. Barty, Anton Mariani, Valerio Tolstikova, Aleksandra Adriano, Luigi Awel, Salah Barthelmess, Miriam Dörner, Katerina Xavier, P. Lourdu Yefanov, Oleksandr James, Daniel R. Nelson, Garrett Wang, Dingjie Calvey, George Chen, Yujie Schmidt, Andrea Szczepek, Michael Frielingsdorf, Stefan Lenz, Oliver Snell, Edward Robinson, Philip J. Šarler, Božidar Belšak, Grega Maček, Marjan Wilde, Fabian Aquila, Andrew Boutet, Sébastien Liang, Mengning Hunter, Mark S. Scheerer, Patrick Lipscomb, John D. Weierstall, Uwe Kornberg, Roger D. Spence, John C. H. Pollack, Lois Chapman, Henry N. Bajt, Saša Sci Rep Article Serial femtosecond crystallography requires reliable and efficient delivery of fresh crystals across the beam of an X-ray free-electron laser over the course of an experiment. We introduce a double-flow focusing nozzle to meet this challenge, with significantly reduced sample consumption, while improving jet stability over previous generations of nozzles. We demonstrate its use to determine the first room-temperature structure of RNA polymerase II at high resolution, revealing new structural details. Moreover, the double flow-focusing nozzles were successfully tested with three other protein samples and the first room temperature structure of an extradiol ring-cleaving dioxygenase was solved by utilizing the improved operation and characteristics of these devices. Nature Publishing Group 2017-03-16 /pmc/articles/PMC5353652/ /pubmed/28300169 http://dx.doi.org/10.1038/srep44628 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
| spellingShingle | Article Oberthuer, Dominik Knoška, Juraj Wiedorn, Max O. Beyerlein, Kenneth R. Bushnell, David A. Kovaleva, Elena G. Heymann, Michael Gumprecht, Lars Kirian, Richard A. Barty, Anton Mariani, Valerio Tolstikova, Aleksandra Adriano, Luigi Awel, Salah Barthelmess, Miriam Dörner, Katerina Xavier, P. Lourdu Yefanov, Oleksandr James, Daniel R. Nelson, Garrett Wang, Dingjie Calvey, George Chen, Yujie Schmidt, Andrea Szczepek, Michael Frielingsdorf, Stefan Lenz, Oliver Snell, Edward Robinson, Philip J. Šarler, Božidar Belšak, Grega Maček, Marjan Wilde, Fabian Aquila, Andrew Boutet, Sébastien Liang, Mengning Hunter, Mark S. Scheerer, Patrick Lipscomb, John D. Weierstall, Uwe Kornberg, Roger D. Spence, John C. H. Pollack, Lois Chapman, Henry N. Bajt, Saša Double-flow focused liquid injector for efficient serial femtosecond crystallography |
| title | Double-flow focused liquid injector for efficient serial femtosecond crystallography |
| title_full | Double-flow focused liquid injector for efficient serial femtosecond crystallography |
| title_fullStr | Double-flow focused liquid injector for efficient serial femtosecond crystallography |
| title_full_unstemmed | Double-flow focused liquid injector for efficient serial femtosecond crystallography |
| title_short | Double-flow focused liquid injector for efficient serial femtosecond crystallography |
| title_sort | double-flow focused liquid injector for efficient serial femtosecond crystallography |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5353652/ https://www.ncbi.nlm.nih.gov/pubmed/28300169 http://dx.doi.org/10.1038/srep44628 |
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