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Should We Perform Semen Analysis, DNA Fragmentation, and Hypo-osmotic Swelling Tests together?

Semen analysis, sperm DNA fragmentation (SDF) and hypo-osmotic swelling test (HOST) are usually performed for the evaluation of sperm fertilizing ability. There are some debates over the necessity of SDF and HOST incorporation in male infertility work-up.Semen of 77 men was evaluated by SDF and HOST...

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Autores principales: Hasanzadeh Keshteli, Samaneh, Farsi, Mir Mehrdad, Khafri, Soraya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Babol University of Medical Sciences 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5353986/
https://www.ncbi.nlm.nih.gov/pubmed/28357201
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author Hasanzadeh Keshteli, Samaneh
Farsi, Mir Mehrdad
Khafri, Soraya
author_facet Hasanzadeh Keshteli, Samaneh
Farsi, Mir Mehrdad
Khafri, Soraya
author_sort Hasanzadeh Keshteli, Samaneh
collection PubMed
description Semen analysis, sperm DNA fragmentation (SDF) and hypo-osmotic swelling test (HOST) are usually performed for the evaluation of sperm fertilizing ability. There are some debates over the necessity of SDF and HOST incorporation in male infertility work-up.Semen of 77 men was evaluated by SDF and HOST through three semen analyses. Sperm parameters were arranged into different categories: <5%, 5-15%, >15% for normal morphology; <50%, 50-70%, >70 % for motility; and <10, 10-20, 21-34, 35-50, >50 million/ml for concentration. SDF analysis was performed and values under 30% were assumed to be normal. Normal range of HOST was considered to be >60%.Only normal sperm morphology had significant relationship with DF rate (P<0.001). Normal morphology, motility, and concentration of sperms had significant relationship with HOST (P<0.001, 0.05, and <0.003,respectively). There was a significant negative correlation between sperm morphology and DF rate. The correlations between sperm parameters and percentage of HOST were significantly positive (r: 0.44, 0.19, and 0.32 for morphology, motility, and concentration, respectively). Receiver operating characteristic curve (ROC) showed that sperm morphology is a strong predictor of the rate of DF and HOST (accuracy: 0.74‚ and 0.81, respectively). The best sperm morphology cut off point for DF and HOST rate prediction was 4.5% and 5.5%, respectively.Sperm morphology had significant correlation with DF rate and HOST and is supposed to be a predictor for these tests. Performing these three tests collectively for evaluation of semen samples would not be necessarily required in all cases.
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spelling pubmed-53539862017-03-29 Should We Perform Semen Analysis, DNA Fragmentation, and Hypo-osmotic Swelling Tests together? Hasanzadeh Keshteli, Samaneh Farsi, Mir Mehrdad Khafri, Soraya Int J Mol Cell Med Original Article Semen analysis, sperm DNA fragmentation (SDF) and hypo-osmotic swelling test (HOST) are usually performed for the evaluation of sperm fertilizing ability. There are some debates over the necessity of SDF and HOST incorporation in male infertility work-up.Semen of 77 men was evaluated by SDF and HOST through three semen analyses. Sperm parameters were arranged into different categories: <5%, 5-15%, >15% for normal morphology; <50%, 50-70%, >70 % for motility; and <10, 10-20, 21-34, 35-50, >50 million/ml for concentration. SDF analysis was performed and values under 30% were assumed to be normal. Normal range of HOST was considered to be >60%.Only normal sperm morphology had significant relationship with DF rate (P<0.001). Normal morphology, motility, and concentration of sperms had significant relationship with HOST (P<0.001, 0.05, and <0.003,respectively). There was a significant negative correlation between sperm morphology and DF rate. The correlations between sperm parameters and percentage of HOST were significantly positive (r: 0.44, 0.19, and 0.32 for morphology, motility, and concentration, respectively). Receiver operating characteristic curve (ROC) showed that sperm morphology is a strong predictor of the rate of DF and HOST (accuracy: 0.74‚ and 0.81, respectively). The best sperm morphology cut off point for DF and HOST rate prediction was 4.5% and 5.5%, respectively.Sperm morphology had significant correlation with DF rate and HOST and is supposed to be a predictor for these tests. Performing these three tests collectively for evaluation of semen samples would not be necessarily required in all cases. Babol University of Medical Sciences 2016 2016-11-13 /pmc/articles/PMC5353986/ /pubmed/28357201 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Hasanzadeh Keshteli, Samaneh
Farsi, Mir Mehrdad
Khafri, Soraya
Should We Perform Semen Analysis, DNA Fragmentation, and Hypo-osmotic Swelling Tests together?
title Should We Perform Semen Analysis, DNA Fragmentation, and Hypo-osmotic Swelling Tests together?
title_full Should We Perform Semen Analysis, DNA Fragmentation, and Hypo-osmotic Swelling Tests together?
title_fullStr Should We Perform Semen Analysis, DNA Fragmentation, and Hypo-osmotic Swelling Tests together?
title_full_unstemmed Should We Perform Semen Analysis, DNA Fragmentation, and Hypo-osmotic Swelling Tests together?
title_short Should We Perform Semen Analysis, DNA Fragmentation, and Hypo-osmotic Swelling Tests together?
title_sort should we perform semen analysis, dna fragmentation, and hypo-osmotic swelling tests together?
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5353986/
https://www.ncbi.nlm.nih.gov/pubmed/28357201
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