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Should We Perform Semen Analysis, DNA Fragmentation, and Hypo-osmotic Swelling Tests together?
Semen analysis, sperm DNA fragmentation (SDF) and hypo-osmotic swelling test (HOST) are usually performed for the evaluation of sperm fertilizing ability. There are some debates over the necessity of SDF and HOST incorporation in male infertility work-up.Semen of 77 men was evaluated by SDF and HOST...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Babol University of Medical Sciences
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5353986/ https://www.ncbi.nlm.nih.gov/pubmed/28357201 |
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author | Hasanzadeh Keshteli, Samaneh Farsi, Mir Mehrdad Khafri, Soraya |
author_facet | Hasanzadeh Keshteli, Samaneh Farsi, Mir Mehrdad Khafri, Soraya |
author_sort | Hasanzadeh Keshteli, Samaneh |
collection | PubMed |
description | Semen analysis, sperm DNA fragmentation (SDF) and hypo-osmotic swelling test (HOST) are usually performed for the evaluation of sperm fertilizing ability. There are some debates over the necessity of SDF and HOST incorporation in male infertility work-up.Semen of 77 men was evaluated by SDF and HOST through three semen analyses. Sperm parameters were arranged into different categories: <5%, 5-15%, >15% for normal morphology; <50%, 50-70%, >70 % for motility; and <10, 10-20, 21-34, 35-50, >50 million/ml for concentration. SDF analysis was performed and values under 30% were assumed to be normal. Normal range of HOST was considered to be >60%.Only normal sperm morphology had significant relationship with DF rate (P<0.001). Normal morphology, motility, and concentration of sperms had significant relationship with HOST (P<0.001, 0.05, and <0.003,respectively). There was a significant negative correlation between sperm morphology and DF rate. The correlations between sperm parameters and percentage of HOST were significantly positive (r: 0.44, 0.19, and 0.32 for morphology, motility, and concentration, respectively). Receiver operating characteristic curve (ROC) showed that sperm morphology is a strong predictor of the rate of DF and HOST (accuracy: 0.74‚ and 0.81, respectively). The best sperm morphology cut off point for DF and HOST rate prediction was 4.5% and 5.5%, respectively.Sperm morphology had significant correlation with DF rate and HOST and is supposed to be a predictor for these tests. Performing these three tests collectively for evaluation of semen samples would not be necessarily required in all cases. |
format | Online Article Text |
id | pubmed-5353986 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Babol University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-53539862017-03-29 Should We Perform Semen Analysis, DNA Fragmentation, and Hypo-osmotic Swelling Tests together? Hasanzadeh Keshteli, Samaneh Farsi, Mir Mehrdad Khafri, Soraya Int J Mol Cell Med Original Article Semen analysis, sperm DNA fragmentation (SDF) and hypo-osmotic swelling test (HOST) are usually performed for the evaluation of sperm fertilizing ability. There are some debates over the necessity of SDF and HOST incorporation in male infertility work-up.Semen of 77 men was evaluated by SDF and HOST through three semen analyses. Sperm parameters were arranged into different categories: <5%, 5-15%, >15% for normal morphology; <50%, 50-70%, >70 % for motility; and <10, 10-20, 21-34, 35-50, >50 million/ml for concentration. SDF analysis was performed and values under 30% were assumed to be normal. Normal range of HOST was considered to be >60%.Only normal sperm morphology had significant relationship with DF rate (P<0.001). Normal morphology, motility, and concentration of sperms had significant relationship with HOST (P<0.001, 0.05, and <0.003,respectively). There was a significant negative correlation between sperm morphology and DF rate. The correlations between sperm parameters and percentage of HOST were significantly positive (r: 0.44, 0.19, and 0.32 for morphology, motility, and concentration, respectively). Receiver operating characteristic curve (ROC) showed that sperm morphology is a strong predictor of the rate of DF and HOST (accuracy: 0.74‚ and 0.81, respectively). The best sperm morphology cut off point for DF and HOST rate prediction was 4.5% and 5.5%, respectively.Sperm morphology had significant correlation with DF rate and HOST and is supposed to be a predictor for these tests. Performing these three tests collectively for evaluation of semen samples would not be necessarily required in all cases. Babol University of Medical Sciences 2016 2016-11-13 /pmc/articles/PMC5353986/ /pubmed/28357201 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Hasanzadeh Keshteli, Samaneh Farsi, Mir Mehrdad Khafri, Soraya Should We Perform Semen Analysis, DNA Fragmentation, and Hypo-osmotic Swelling Tests together? |
title | Should We Perform Semen Analysis, DNA Fragmentation, and Hypo-osmotic Swelling Tests together? |
title_full | Should We Perform Semen Analysis, DNA Fragmentation, and Hypo-osmotic Swelling Tests together? |
title_fullStr | Should We Perform Semen Analysis, DNA Fragmentation, and Hypo-osmotic Swelling Tests together? |
title_full_unstemmed | Should We Perform Semen Analysis, DNA Fragmentation, and Hypo-osmotic Swelling Tests together? |
title_short | Should We Perform Semen Analysis, DNA Fragmentation, and Hypo-osmotic Swelling Tests together? |
title_sort | should we perform semen analysis, dna fragmentation, and hypo-osmotic swelling tests together? |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5353986/ https://www.ncbi.nlm.nih.gov/pubmed/28357201 |
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