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NicoLase—An open-source diode laser combiner, fiber launch, and sequencing controller for fluorescence microscopy

Modern fluorescence microscopy requires software-controlled illumination sources with high power across a wide range of wavelengths. Diode lasers meet the power requirements and combining multiple units into a single fiber launch expands their capability across the required spectral range. We presen...

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Detalles Bibliográficos
Autores principales: Nicovich, Philip R., Walsh, James, Böcking, Till, Gaus, Katharina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5354410/
https://www.ncbi.nlm.nih.gov/pubmed/28301563
http://dx.doi.org/10.1371/journal.pone.0173879
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author Nicovich, Philip R.
Walsh, James
Böcking, Till
Gaus, Katharina
author_facet Nicovich, Philip R.
Walsh, James
Böcking, Till
Gaus, Katharina
author_sort Nicovich, Philip R.
collection PubMed
description Modern fluorescence microscopy requires software-controlled illumination sources with high power across a wide range of wavelengths. Diode lasers meet the power requirements and combining multiple units into a single fiber launch expands their capability across the required spectral range. We present the NicoLase, an open-source diode laser combiner, fiber launch, and software sequence controller for fluorescence microscopy and super-resolution microscopy applications. Two configurations are described, giving four or six output wavelengths and one or two single-mode fiber outputs, with all CAD files, machinist drawings, and controller source code openly available.
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spelling pubmed-53544102017-04-06 NicoLase—An open-source diode laser combiner, fiber launch, and sequencing controller for fluorescence microscopy Nicovich, Philip R. Walsh, James Böcking, Till Gaus, Katharina PLoS One Research Article Modern fluorescence microscopy requires software-controlled illumination sources with high power across a wide range of wavelengths. Diode lasers meet the power requirements and combining multiple units into a single fiber launch expands their capability across the required spectral range. We present the NicoLase, an open-source diode laser combiner, fiber launch, and software sequence controller for fluorescence microscopy and super-resolution microscopy applications. Two configurations are described, giving four or six output wavelengths and one or two single-mode fiber outputs, with all CAD files, machinist drawings, and controller source code openly available. Public Library of Science 2017-03-16 /pmc/articles/PMC5354410/ /pubmed/28301563 http://dx.doi.org/10.1371/journal.pone.0173879 Text en © 2017 Nicovich et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Nicovich, Philip R.
Walsh, James
Böcking, Till
Gaus, Katharina
NicoLase—An open-source diode laser combiner, fiber launch, and sequencing controller for fluorescence microscopy
title NicoLase—An open-source diode laser combiner, fiber launch, and sequencing controller for fluorescence microscopy
title_full NicoLase—An open-source diode laser combiner, fiber launch, and sequencing controller for fluorescence microscopy
title_fullStr NicoLase—An open-source diode laser combiner, fiber launch, and sequencing controller for fluorescence microscopy
title_full_unstemmed NicoLase—An open-source diode laser combiner, fiber launch, and sequencing controller for fluorescence microscopy
title_short NicoLase—An open-source diode laser combiner, fiber launch, and sequencing controller for fluorescence microscopy
title_sort nicolase—an open-source diode laser combiner, fiber launch, and sequencing controller for fluorescence microscopy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5354410/
https://www.ncbi.nlm.nih.gov/pubmed/28301563
http://dx.doi.org/10.1371/journal.pone.0173879
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