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Quantitative proteomics reveals FLNC as a potential progression marker for the development of hepatocellular carcinoma
Hepatocellular carcinoma (HCC) caused by hepatitis B virus (HBV) infection is one of the most life-threatening human cancers in China. However, the pathogenesis of HCC development is still unclear. Here, we systemically analyzed liver tissues from different stages of HCC patients through 8-plex Isob...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5354476/ https://www.ncbi.nlm.nih.gov/pubmed/27626164 http://dx.doi.org/10.18632/oncotarget.11921 |
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author | Qi, Yingzi Xu, Feng Chen, Lingsheng Li, Yanchang Xu, Zhongwei Zhang, Yao Wei, Wei Su, Na Zhang, Tao Fan, Fengxu Wang, Xing Qin, Xue Zhang, Lingqiang Liu, Yinkun Xu, Ping |
author_facet | Qi, Yingzi Xu, Feng Chen, Lingsheng Li, Yanchang Xu, Zhongwei Zhang, Yao Wei, Wei Su, Na Zhang, Tao Fan, Fengxu Wang, Xing Qin, Xue Zhang, Lingqiang Liu, Yinkun Xu, Ping |
author_sort | Qi, Yingzi |
collection | PubMed |
description | Hepatocellular carcinoma (HCC) caused by hepatitis B virus (HBV) infection is one of the most life-threatening human cancers in China. However, the pathogenesis of HCC development is still unclear. Here, we systemically analyzed liver tissues from different stages of HCC patients through 8-plex Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) approach. A total of 4,620 proteins were identified and 3,781 proteins were quantified. When T1, T2 and T3 tumor tissues were compared with T1 non-tumor cells, 330, 365 and 387 differentially expressed proteins were identified respectively. IPA (Ingenuity Pathway Analysis) analysis revealed that these differentially expressed proteins were involved in endothelial cancer, cell spreading, cell adhesion and cell movement of tumor cell lines pathway and so on. Further study showed that the filamin C (FLNC) protein was significantly overexpressed with the development of HCC, which might play an important role in HCC invasion and metastasis. These results were also confirmed with western blot (WB). The mRNA levels were significantly increased in 50 pairs of tumor and adjacent non-tumor tissues from TCGA database. The higher expression of FLNC in HCC might be a common phenomenon, thereby shedding new light on molecular mechanism and biomarker for the diagnosis purpose of HCC development. |
format | Online Article Text |
id | pubmed-5354476 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-53544762017-03-24 Quantitative proteomics reveals FLNC as a potential progression marker for the development of hepatocellular carcinoma Qi, Yingzi Xu, Feng Chen, Lingsheng Li, Yanchang Xu, Zhongwei Zhang, Yao Wei, Wei Su, Na Zhang, Tao Fan, Fengxu Wang, Xing Qin, Xue Zhang, Lingqiang Liu, Yinkun Xu, Ping Oncotarget Research Paper Hepatocellular carcinoma (HCC) caused by hepatitis B virus (HBV) infection is one of the most life-threatening human cancers in China. However, the pathogenesis of HCC development is still unclear. Here, we systemically analyzed liver tissues from different stages of HCC patients through 8-plex Isobaric Tags for Relative and Absolute Quantitation (iTRAQ) approach. A total of 4,620 proteins were identified and 3,781 proteins were quantified. When T1, T2 and T3 tumor tissues were compared with T1 non-tumor cells, 330, 365 and 387 differentially expressed proteins were identified respectively. IPA (Ingenuity Pathway Analysis) analysis revealed that these differentially expressed proteins were involved in endothelial cancer, cell spreading, cell adhesion and cell movement of tumor cell lines pathway and so on. Further study showed that the filamin C (FLNC) protein was significantly overexpressed with the development of HCC, which might play an important role in HCC invasion and metastasis. These results were also confirmed with western blot (WB). The mRNA levels were significantly increased in 50 pairs of tumor and adjacent non-tumor tissues from TCGA database. The higher expression of FLNC in HCC might be a common phenomenon, thereby shedding new light on molecular mechanism and biomarker for the diagnosis purpose of HCC development. Impact Journals LLC 2016-09-09 /pmc/articles/PMC5354476/ /pubmed/27626164 http://dx.doi.org/10.18632/oncotarget.11921 Text en Copyright: © 2016 Qi et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Qi, Yingzi Xu, Feng Chen, Lingsheng Li, Yanchang Xu, Zhongwei Zhang, Yao Wei, Wei Su, Na Zhang, Tao Fan, Fengxu Wang, Xing Qin, Xue Zhang, Lingqiang Liu, Yinkun Xu, Ping Quantitative proteomics reveals FLNC as a potential progression marker for the development of hepatocellular carcinoma |
title | Quantitative proteomics reveals FLNC as a potential progression marker for the development of hepatocellular carcinoma |
title_full | Quantitative proteomics reveals FLNC as a potential progression marker for the development of hepatocellular carcinoma |
title_fullStr | Quantitative proteomics reveals FLNC as a potential progression marker for the development of hepatocellular carcinoma |
title_full_unstemmed | Quantitative proteomics reveals FLNC as a potential progression marker for the development of hepatocellular carcinoma |
title_short | Quantitative proteomics reveals FLNC as a potential progression marker for the development of hepatocellular carcinoma |
title_sort | quantitative proteomics reveals flnc as a potential progression marker for the development of hepatocellular carcinoma |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5354476/ https://www.ncbi.nlm.nih.gov/pubmed/27626164 http://dx.doi.org/10.18632/oncotarget.11921 |
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