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Autophosphorylation-based Calcium (Ca(2+)) Sensitivity Priming and Ca(2+)/Calmodulin Inhibition of Arabidopsis thaliana Ca(2+)-dependent Protein Kinase 28 (CPK28)

Plant calcium (Ca(2+))-dependent protein kinases (CPKs) represent the primary Ca(2+)-dependent protein kinase activities in plant systems. CPKs are composed of a dual specificity (Ser/Thr and Tyr) kinase domain tethered to a calmodulin-like domain (CLD) via an autoinhibitory junction (J). Although r...

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Autores principales: Bender, Kyle W., Blackburn, R. Kevin, Monaghan, Jacqueline, Derbyshire, Paul, Menke, Frank L. H., Zipfel, Cyril, Goshe, Michael B., Zielinski, Raymond E., Huber, Steven C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5354511/
https://www.ncbi.nlm.nih.gov/pubmed/28154194
http://dx.doi.org/10.1074/jbc.M116.763243
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author Bender, Kyle W.
Blackburn, R. Kevin
Monaghan, Jacqueline
Derbyshire, Paul
Menke, Frank L. H.
Zipfel, Cyril
Goshe, Michael B.
Zielinski, Raymond E.
Huber, Steven C.
author_facet Bender, Kyle W.
Blackburn, R. Kevin
Monaghan, Jacqueline
Derbyshire, Paul
Menke, Frank L. H.
Zipfel, Cyril
Goshe, Michael B.
Zielinski, Raymond E.
Huber, Steven C.
author_sort Bender, Kyle W.
collection PubMed
description Plant calcium (Ca(2+))-dependent protein kinases (CPKs) represent the primary Ca(2+)-dependent protein kinase activities in plant systems. CPKs are composed of a dual specificity (Ser/Thr and Tyr) kinase domain tethered to a calmodulin-like domain (CLD) via an autoinhibitory junction (J). Although regulation of CPKs by Ca(2+) has been extensively studied, the contribution of autophosphorylation in controlling CPK activity is less well understood. Furthermore, whether calmodulin (CaM) contributes to CPK regulation, as is the case for Ca(2+)/CaM-dependent protein kinases outside the plant lineage, remains an open question. We therefore screened a subset of plant CPKs for CaM binding and found that CPK28 is a high affinity Ca(2+)/CaM-binding protein. Using synthetic peptides and native gel electrophoresis, we coarsely mapped the CaM-binding domain to a site within the CPK28 J domain that overlaps with the known site of intramolecular interaction between the J domain and the CLD. Peptide kinase activity of fully dephosphorylated CPK28 was Ca(2+)-responsive and was inhibited by Ca(2+)/CaM. Using in situ autophosphorylated protein, we expand on the known set of CPK28 autophosphorylation sites, and we demonstrate that, unexpectedly, autophosphorylated CPK28 had enhanced kinase activity at physiological concentrations of Ca(2+) compared with the dephosphorylated protein, suggesting that autophosphorylation functions to prime CPK28 for Ca(2+) activation and might also allow CPK28 to remain active when Ca(2+) levels are low. Furthermore, CPK28 autophosphorylation substantially reduced sensitivity of the kinase to Ca(2+)/CaM inhibition. Overall, our analyses uncover new complexities in the control of CPK28 and provide mechanistic support for Ca(2+) signaling specificity through Ca(2+) sensor priming.
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spelling pubmed-53545112017-03-23 Autophosphorylation-based Calcium (Ca(2+)) Sensitivity Priming and Ca(2+)/Calmodulin Inhibition of Arabidopsis thaliana Ca(2+)-dependent Protein Kinase 28 (CPK28) Bender, Kyle W. Blackburn, R. Kevin Monaghan, Jacqueline Derbyshire, Paul Menke, Frank L. H. Zipfel, Cyril Goshe, Michael B. Zielinski, Raymond E. Huber, Steven C. J Biol Chem Editors' Picks Plant calcium (Ca(2+))-dependent protein kinases (CPKs) represent the primary Ca(2+)-dependent protein kinase activities in plant systems. CPKs are composed of a dual specificity (Ser/Thr and Tyr) kinase domain tethered to a calmodulin-like domain (CLD) via an autoinhibitory junction (J). Although regulation of CPKs by Ca(2+) has been extensively studied, the contribution of autophosphorylation in controlling CPK activity is less well understood. Furthermore, whether calmodulin (CaM) contributes to CPK regulation, as is the case for Ca(2+)/CaM-dependent protein kinases outside the plant lineage, remains an open question. We therefore screened a subset of plant CPKs for CaM binding and found that CPK28 is a high affinity Ca(2+)/CaM-binding protein. Using synthetic peptides and native gel electrophoresis, we coarsely mapped the CaM-binding domain to a site within the CPK28 J domain that overlaps with the known site of intramolecular interaction between the J domain and the CLD. Peptide kinase activity of fully dephosphorylated CPK28 was Ca(2+)-responsive and was inhibited by Ca(2+)/CaM. Using in situ autophosphorylated protein, we expand on the known set of CPK28 autophosphorylation sites, and we demonstrate that, unexpectedly, autophosphorylated CPK28 had enhanced kinase activity at physiological concentrations of Ca(2+) compared with the dephosphorylated protein, suggesting that autophosphorylation functions to prime CPK28 for Ca(2+) activation and might also allow CPK28 to remain active when Ca(2+) levels are low. Furthermore, CPK28 autophosphorylation substantially reduced sensitivity of the kinase to Ca(2+)/CaM inhibition. Overall, our analyses uncover new complexities in the control of CPK28 and provide mechanistic support for Ca(2+) signaling specificity through Ca(2+) sensor priming. American Society for Biochemistry and Molecular Biology 2017-03-10 2017-01-30 /pmc/articles/PMC5354511/ /pubmed/28154194 http://dx.doi.org/10.1074/jbc.M116.763243 Text en © 2017 by The American Society for Biochemistry and Molecular Biology, Inc. Author's Choice—Final version free via Creative Commons CC-BY license (http://creativecommons.org/licenses/by/4.0) .
spellingShingle Editors' Picks
Bender, Kyle W.
Blackburn, R. Kevin
Monaghan, Jacqueline
Derbyshire, Paul
Menke, Frank L. H.
Zipfel, Cyril
Goshe, Michael B.
Zielinski, Raymond E.
Huber, Steven C.
Autophosphorylation-based Calcium (Ca(2+)) Sensitivity Priming and Ca(2+)/Calmodulin Inhibition of Arabidopsis thaliana Ca(2+)-dependent Protein Kinase 28 (CPK28)
title Autophosphorylation-based Calcium (Ca(2+)) Sensitivity Priming and Ca(2+)/Calmodulin Inhibition of Arabidopsis thaliana Ca(2+)-dependent Protein Kinase 28 (CPK28)
title_full Autophosphorylation-based Calcium (Ca(2+)) Sensitivity Priming and Ca(2+)/Calmodulin Inhibition of Arabidopsis thaliana Ca(2+)-dependent Protein Kinase 28 (CPK28)
title_fullStr Autophosphorylation-based Calcium (Ca(2+)) Sensitivity Priming and Ca(2+)/Calmodulin Inhibition of Arabidopsis thaliana Ca(2+)-dependent Protein Kinase 28 (CPK28)
title_full_unstemmed Autophosphorylation-based Calcium (Ca(2+)) Sensitivity Priming and Ca(2+)/Calmodulin Inhibition of Arabidopsis thaliana Ca(2+)-dependent Protein Kinase 28 (CPK28)
title_short Autophosphorylation-based Calcium (Ca(2+)) Sensitivity Priming and Ca(2+)/Calmodulin Inhibition of Arabidopsis thaliana Ca(2+)-dependent Protein Kinase 28 (CPK28)
title_sort autophosphorylation-based calcium (ca(2+)) sensitivity priming and ca(2+)/calmodulin inhibition of arabidopsis thaliana ca(2+)-dependent protein kinase 28 (cpk28)
topic Editors' Picks
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5354511/
https://www.ncbi.nlm.nih.gov/pubmed/28154194
http://dx.doi.org/10.1074/jbc.M116.763243
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