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Antibiotic resistance among Helicobacter pylori clinical isolates in Lima, Peru
OBJECTIVES: Gastric carcinoma is the most common cancer and cause of cancer mortality in Peru. Helicobacter pylori, a bacterium that colonizes the human stomach, is a Group 1 carcinogen due to its causal relationship to gastric carcinoma. While eradication of H. pylori can help prevent gastric cance...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5354526/ https://www.ncbi.nlm.nih.gov/pubmed/28331349 http://dx.doi.org/10.2147/IDR.S123798 |
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author | Boehnke, Kevin F Valdivieso, Manuel Bussalleu, Alejandro Sexton, Rachael Thompson, Kathryn C Osorio, Soledad Reyes, Italo Novoa Crowley, John J Baker, Laurence H Xi, Chuanwu |
author_facet | Boehnke, Kevin F Valdivieso, Manuel Bussalleu, Alejandro Sexton, Rachael Thompson, Kathryn C Osorio, Soledad Reyes, Italo Novoa Crowley, John J Baker, Laurence H Xi, Chuanwu |
author_sort | Boehnke, Kevin F |
collection | PubMed |
description | OBJECTIVES: Gastric carcinoma is the most common cancer and cause of cancer mortality in Peru. Helicobacter pylori, a bacterium that colonizes the human stomach, is a Group 1 carcinogen due to its causal relationship to gastric carcinoma. While eradication of H. pylori can help prevent gastric cancer, characterizing regional antibiotic resistance patterns is necessary to determine targeted treatment for each region. Thus, we examined primary antibiotic resistance in clinical isolates of H. pylori in Lima, Peru. MATERIALS AND METHODS: H. pylori strains were isolated from gastric biopsies of patients with histologically proven H. pylori infection. Primary antibiotic resistance among isolates was examined using E-test strips. Isolates were examined for the presence of the cagA pathogenicity island and the vacA m1/m2 alleles via polymerase chain reaction. RESULTS: Seventy-six isolates were recovered from gastric biopsies. Clinical isolates showed evidence of antibiotic resistance to 1 (27.6%, n=21/76), 2 (28.9%, n=22/76), or ≥3 antibiotics (40.8%). Of 76 isolates, eight (10.5%) were resistant to amoxicillin and clarithromycin, which are part of the standard triple therapy for H. pylori infection. No trends were seen between the presence of cagA, vacA m1, or vacA m2 and antibiotic resistance. CONCLUSION: The rate of antibiotic resistance among H. pylori isolates in Lima, Peru, is higher than expected and presents cause for concern. To develop more targeted eradication therapies for H. pylori in Peru, more research is needed to better characterize antibiotic resistance among a larger number of clinical isolates prospectively. |
format | Online Article Text |
id | pubmed-5354526 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-53545262017-03-22 Antibiotic resistance among Helicobacter pylori clinical isolates in Lima, Peru Boehnke, Kevin F Valdivieso, Manuel Bussalleu, Alejandro Sexton, Rachael Thompson, Kathryn C Osorio, Soledad Reyes, Italo Novoa Crowley, John J Baker, Laurence H Xi, Chuanwu Infect Drug Resist Original Research OBJECTIVES: Gastric carcinoma is the most common cancer and cause of cancer mortality in Peru. Helicobacter pylori, a bacterium that colonizes the human stomach, is a Group 1 carcinogen due to its causal relationship to gastric carcinoma. While eradication of H. pylori can help prevent gastric cancer, characterizing regional antibiotic resistance patterns is necessary to determine targeted treatment for each region. Thus, we examined primary antibiotic resistance in clinical isolates of H. pylori in Lima, Peru. MATERIALS AND METHODS: H. pylori strains were isolated from gastric biopsies of patients with histologically proven H. pylori infection. Primary antibiotic resistance among isolates was examined using E-test strips. Isolates were examined for the presence of the cagA pathogenicity island and the vacA m1/m2 alleles via polymerase chain reaction. RESULTS: Seventy-six isolates were recovered from gastric biopsies. Clinical isolates showed evidence of antibiotic resistance to 1 (27.6%, n=21/76), 2 (28.9%, n=22/76), or ≥3 antibiotics (40.8%). Of 76 isolates, eight (10.5%) were resistant to amoxicillin and clarithromycin, which are part of the standard triple therapy for H. pylori infection. No trends were seen between the presence of cagA, vacA m1, or vacA m2 and antibiotic resistance. CONCLUSION: The rate of antibiotic resistance among H. pylori isolates in Lima, Peru, is higher than expected and presents cause for concern. To develop more targeted eradication therapies for H. pylori in Peru, more research is needed to better characterize antibiotic resistance among a larger number of clinical isolates prospectively. Dove Medical Press 2017-03-10 /pmc/articles/PMC5354526/ /pubmed/28331349 http://dx.doi.org/10.2147/IDR.S123798 Text en © 2017 Boehnke et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Boehnke, Kevin F Valdivieso, Manuel Bussalleu, Alejandro Sexton, Rachael Thompson, Kathryn C Osorio, Soledad Reyes, Italo Novoa Crowley, John J Baker, Laurence H Xi, Chuanwu Antibiotic resistance among Helicobacter pylori clinical isolates in Lima, Peru |
title | Antibiotic resistance among Helicobacter pylori clinical isolates in Lima, Peru |
title_full | Antibiotic resistance among Helicobacter pylori clinical isolates in Lima, Peru |
title_fullStr | Antibiotic resistance among Helicobacter pylori clinical isolates in Lima, Peru |
title_full_unstemmed | Antibiotic resistance among Helicobacter pylori clinical isolates in Lima, Peru |
title_short | Antibiotic resistance among Helicobacter pylori clinical isolates in Lima, Peru |
title_sort | antibiotic resistance among helicobacter pylori clinical isolates in lima, peru |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5354526/ https://www.ncbi.nlm.nih.gov/pubmed/28331349 http://dx.doi.org/10.2147/IDR.S123798 |
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