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At neutral pH the chronological lifespan of Hansenula polymorpha increases upon enhancing the carbon source concentrations

Dietary restriction is generally assumed to increase the lifespan in most eukaryotes, including the simple model organism Saccharomyces cerevisiae. However, recent data questioned whether this phenomenon is indeed true for yeast. We studied the effect of reduction of the carbon source concentration...

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Autores principales: Kawałek, Adam, van der Klei, Ida J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Shared Science Publishers OG 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5354561/
https://www.ncbi.nlm.nih.gov/pubmed/28357243
http://dx.doi.org/10.15698/mic2014.06.149
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author Kawałek, Adam
van der Klei, Ida J.
author_facet Kawałek, Adam
van der Klei, Ida J.
author_sort Kawałek, Adam
collection PubMed
description Dietary restriction is generally assumed to increase the lifespan in most eukaryotes, including the simple model organism Saccharomyces cerevisiae. However, recent data questioned whether this phenomenon is indeed true for yeast. We studied the effect of reduction of the carbon source concentration on the chronological lifespan of the yeast Hansenula polymorpha using four different carbon sources. Our data indicate that reduction of the carbon source concentration has a negative (glucose, ethanol, methanol) or positive (glycerol) effect on the chronological lifespan. We show that the actual effect of carbon source concentrations largely depends on extracellular factor(s). We provide evidence that H. polymorpha acidifies the medium and that a low pH of the medium alone is sufficient to significantly decrease the chronological lifespan. However, glucose-grown cells are less sensitive to low pH compared to glycerol-grown cells, explaining why only the reduction of the glycerol-concentration (which leads to less medium acidification) has a positive effect on the chronological lifespan. Instead, the positive effect of enhancing the glucose concentrations is much larger than the negative effect of the medium acidification at these conditions, explaining the increased lifespan with increasing glucose concentrations. Importantly, at neutral pH, the chronological lifespan also decreases with a reduction in glycerol concentrations. We show that for glycerol cultures this effect is related to acidification independent changes in the composition of the spent medium. Altogether, our data indicate that in H. polymorpha at neutral pH the chronological lifespan invariably extends upon increasing the carbon source concentration.
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spelling pubmed-53545612017-03-29 At neutral pH the chronological lifespan of Hansenula polymorpha increases upon enhancing the carbon source concentrations Kawałek, Adam van der Klei, Ida J. Microb Cell Microbiology Dietary restriction is generally assumed to increase the lifespan in most eukaryotes, including the simple model organism Saccharomyces cerevisiae. However, recent data questioned whether this phenomenon is indeed true for yeast. We studied the effect of reduction of the carbon source concentration on the chronological lifespan of the yeast Hansenula polymorpha using four different carbon sources. Our data indicate that reduction of the carbon source concentration has a negative (glucose, ethanol, methanol) or positive (glycerol) effect on the chronological lifespan. We show that the actual effect of carbon source concentrations largely depends on extracellular factor(s). We provide evidence that H. polymorpha acidifies the medium and that a low pH of the medium alone is sufficient to significantly decrease the chronological lifespan. However, glucose-grown cells are less sensitive to low pH compared to glycerol-grown cells, explaining why only the reduction of the glycerol-concentration (which leads to less medium acidification) has a positive effect on the chronological lifespan. Instead, the positive effect of enhancing the glucose concentrations is much larger than the negative effect of the medium acidification at these conditions, explaining the increased lifespan with increasing glucose concentrations. Importantly, at neutral pH, the chronological lifespan also decreases with a reduction in glycerol concentrations. We show that for glycerol cultures this effect is related to acidification independent changes in the composition of the spent medium. Altogether, our data indicate that in H. polymorpha at neutral pH the chronological lifespan invariably extends upon increasing the carbon source concentration. Shared Science Publishers OG 2014-05-20 /pmc/articles/PMC5354561/ /pubmed/28357243 http://dx.doi.org/10.15698/mic2014.06.149 Text en https://creativecommons.org/licenses/by/4.0/ This is an open-access article released under the terms of the Creative Commons Attribution (CC BY) license, which allows the unrestricted use, distribution, and reproduction in any medium, provided the original author and source are acknowledged.
spellingShingle Microbiology
Kawałek, Adam
van der Klei, Ida J.
At neutral pH the chronological lifespan of Hansenula polymorpha increases upon enhancing the carbon source concentrations
title At neutral pH the chronological lifespan of Hansenula polymorpha increases upon enhancing the carbon source concentrations
title_full At neutral pH the chronological lifespan of Hansenula polymorpha increases upon enhancing the carbon source concentrations
title_fullStr At neutral pH the chronological lifespan of Hansenula polymorpha increases upon enhancing the carbon source concentrations
title_full_unstemmed At neutral pH the chronological lifespan of Hansenula polymorpha increases upon enhancing the carbon source concentrations
title_short At neutral pH the chronological lifespan of Hansenula polymorpha increases upon enhancing the carbon source concentrations
title_sort at neutral ph the chronological lifespan of hansenula polymorpha increases upon enhancing the carbon source concentrations
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5354561/
https://www.ncbi.nlm.nih.gov/pubmed/28357243
http://dx.doi.org/10.15698/mic2014.06.149
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