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Cox1 mutation abrogates need for Cox23 in cytochrome c oxidase biogenesis

Cox23 is a known conserved assembly factor for cytochrome c oxidase, although its role in cytochrome c oxidase (CcO) biogenesis remains unresolved. To gain additional insights into its role, we isolated spontaneous suppressors of the respiratory growth defect in cox23∆ yeast cells. We recovered inde...

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Autores principales: Dela Cruz, Richard, Jeong, Mi-Young, Winge, Dennis R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Shared Science Publishers OG 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5354592/
https://www.ncbi.nlm.nih.gov/pubmed/28357365
http://dx.doi.org/10.15698/mic2016.07.511
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author Dela Cruz, Richard
Jeong, Mi-Young
Winge, Dennis R.
author_facet Dela Cruz, Richard
Jeong, Mi-Young
Winge, Dennis R.
author_sort Dela Cruz, Richard
collection PubMed
description Cox23 is a known conserved assembly factor for cytochrome c oxidase, although its role in cytochrome c oxidase (CcO) biogenesis remains unresolved. To gain additional insights into its role, we isolated spontaneous suppressors of the respiratory growth defect in cox23∆ yeast cells. We recovered independent colonies that propagated on glycerol/lactate medium for cox23∆ cells at 37°C. We mapped these mutations to the mitochondrial genome and specifically to COX1 yielding an I(101)F substitution. The I(101)F Cox1 allele is a gain-of-function mutation enabling yeast to respire in the absence of Cox23. CcO subunit steady-state levels were restored with the I(101)F Cox1 suppressor mutation and oxygen consumption and CcO activity were likewise restored. Cells harboring the mitochondrial genome encoding I(101)F Cox1 were used to delete genes for other CcO assembly factors to test the specificity of the Cox1 mutation as a suppressor of cox23∆ cells. The Cox1 mutant allele fails to support respiratory growth in yeast lacking Cox17, Cox19, Coa1, Coa2, Cox14 or Shy1, demonstrating its specific suppressor activity for cox23∆ cells.
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spelling pubmed-53545922017-03-29 Cox1 mutation abrogates need for Cox23 in cytochrome c oxidase biogenesis Dela Cruz, Richard Jeong, Mi-Young Winge, Dennis R. Microb Cell Microbiology Cox23 is a known conserved assembly factor for cytochrome c oxidase, although its role in cytochrome c oxidase (CcO) biogenesis remains unresolved. To gain additional insights into its role, we isolated spontaneous suppressors of the respiratory growth defect in cox23∆ yeast cells. We recovered independent colonies that propagated on glycerol/lactate medium for cox23∆ cells at 37°C. We mapped these mutations to the mitochondrial genome and specifically to COX1 yielding an I(101)F substitution. The I(101)F Cox1 allele is a gain-of-function mutation enabling yeast to respire in the absence of Cox23. CcO subunit steady-state levels were restored with the I(101)F Cox1 suppressor mutation and oxygen consumption and CcO activity were likewise restored. Cells harboring the mitochondrial genome encoding I(101)F Cox1 were used to delete genes for other CcO assembly factors to test the specificity of the Cox1 mutation as a suppressor of cox23∆ cells. The Cox1 mutant allele fails to support respiratory growth in yeast lacking Cox17, Cox19, Coa1, Coa2, Cox14 or Shy1, demonstrating its specific suppressor activity for cox23∆ cells. Shared Science Publishers OG 2016-06-30 /pmc/articles/PMC5354592/ /pubmed/28357365 http://dx.doi.org/10.15698/mic2016.07.511 Text en https://creativecommons.org/licenses/by/4.0/ This is an open-access article released under the terms of the Creative Commons Attribution (CC BY) license, which allows the unrestricted use, distribution, and reproduction in any medium, provided the original author and source are acknowledged.
spellingShingle Microbiology
Dela Cruz, Richard
Jeong, Mi-Young
Winge, Dennis R.
Cox1 mutation abrogates need for Cox23 in cytochrome c oxidase biogenesis
title Cox1 mutation abrogates need for Cox23 in cytochrome c oxidase biogenesis
title_full Cox1 mutation abrogates need for Cox23 in cytochrome c oxidase biogenesis
title_fullStr Cox1 mutation abrogates need for Cox23 in cytochrome c oxidase biogenesis
title_full_unstemmed Cox1 mutation abrogates need for Cox23 in cytochrome c oxidase biogenesis
title_short Cox1 mutation abrogates need for Cox23 in cytochrome c oxidase biogenesis
title_sort cox1 mutation abrogates need for cox23 in cytochrome c oxidase biogenesis
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5354592/
https://www.ncbi.nlm.nih.gov/pubmed/28357365
http://dx.doi.org/10.15698/mic2016.07.511
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