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Seasonality of sand flies (Diptera: Psychodidae) and Leishmania DNA detection in vector species in an area with endemic visceral leishmaniasis
BACKGROUND: Leishmaniases are a serious health problem in southeast Brazil, including the city of Belo Horizonte (BH), Minas Gerais state (MG), where there are high rates of incidence and mortality due to visceral leishmaniases. BH is divided into nine sanitary districts (SD) of which one, the Venda...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Instituto Oswaldo Cruz, Ministério da Saúde
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5354617/ https://www.ncbi.nlm.nih.gov/pubmed/28327794 http://dx.doi.org/10.1590/0074-02760160438 |
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author | Saraiva, Lara Leite, Camila Gonçalves Lima, Ana Cristina Vianna Mariano da Rocha de Carvalho, Luiz Otávio Alves Pereira, Agnes Antônia Sampaio Rugani, Jerônimo Marteleto Nunes Rego, Felipe Dutra Gontijo, Célia Maria Ferreira Andrade, José Dilermando |
author_facet | Saraiva, Lara Leite, Camila Gonçalves Lima, Ana Cristina Vianna Mariano da Rocha de Carvalho, Luiz Otávio Alves Pereira, Agnes Antônia Sampaio Rugani, Jerônimo Marteleto Nunes Rego, Felipe Dutra Gontijo, Célia Maria Ferreira Andrade, José Dilermando |
author_sort | Saraiva, Lara |
collection | PubMed |
description | BACKGROUND: Leishmaniases are a serious health problem in southeast Brazil, including the city of Belo Horizonte (BH), Minas Gerais state (MG), where there are high rates of incidence and mortality due to visceral leishmaniases. BH is divided into nine sanitary districts (SD) of which one, the Venda Nova SD, was selected for this study because it has high rates of positivity for canine leishmaniasis and high incidence of human leishmaniasis. OBJECTIVES: This study aimed to survey the sand fly fauna in Venda Nova SD from August 2011 to July 2013 and perform a descriptive analysis of the vector population. METHODS: The sampling was carried out using automatic HP light traps at all covered areas of the Venda Nova SD, in a total of eighteen light traps. Sampled specimens were identified following Galati (2003), and females were submitted to molecular techniques for the detection and identification of Leishmania DNA. A simple environmental description was done for it area and Kernel estimation was used to infer vector density for each study site. FINDINGS: A total of 2,427 sand fly specimens belonging to eight species and five genera were collected of which 95.3% were Lutzomyia longipalpis. The seasonal variation curve was delineated by this species. Lu. longipalpis was the most abundant at all collection points and in all months of the study, and exhibited a natural infection rate of 1.01% for Leishmania infantum and 1.77% for Leishmania braziliensis. MAIN CONCLUSIONS: The results show the presence and adaptation of Lu. longipalpis to the anthropic environment of BH and reinforces its role as the main vector of L. infantum in the region. |
format | Online Article Text |
id | pubmed-5354617 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Instituto Oswaldo Cruz, Ministério da Saúde |
record_format | MEDLINE/PubMed |
spelling | pubmed-53546172017-04-01 Seasonality of sand flies (Diptera: Psychodidae) and Leishmania DNA detection in vector species in an area with endemic visceral leishmaniasis Saraiva, Lara Leite, Camila Gonçalves Lima, Ana Cristina Vianna Mariano da Rocha de Carvalho, Luiz Otávio Alves Pereira, Agnes Antônia Sampaio Rugani, Jerônimo Marteleto Nunes Rego, Felipe Dutra Gontijo, Célia Maria Ferreira Andrade, José Dilermando Mem Inst Oswaldo Cruz Articles BACKGROUND: Leishmaniases are a serious health problem in southeast Brazil, including the city of Belo Horizonte (BH), Minas Gerais state (MG), where there are high rates of incidence and mortality due to visceral leishmaniases. BH is divided into nine sanitary districts (SD) of which one, the Venda Nova SD, was selected for this study because it has high rates of positivity for canine leishmaniasis and high incidence of human leishmaniasis. OBJECTIVES: This study aimed to survey the sand fly fauna in Venda Nova SD from August 2011 to July 2013 and perform a descriptive analysis of the vector population. METHODS: The sampling was carried out using automatic HP light traps at all covered areas of the Venda Nova SD, in a total of eighteen light traps. Sampled specimens were identified following Galati (2003), and females were submitted to molecular techniques for the detection and identification of Leishmania DNA. A simple environmental description was done for it area and Kernel estimation was used to infer vector density for each study site. FINDINGS: A total of 2,427 sand fly specimens belonging to eight species and five genera were collected of which 95.3% were Lutzomyia longipalpis. The seasonal variation curve was delineated by this species. Lu. longipalpis was the most abundant at all collection points and in all months of the study, and exhibited a natural infection rate of 1.01% for Leishmania infantum and 1.77% for Leishmania braziliensis. MAIN CONCLUSIONS: The results show the presence and adaptation of Lu. longipalpis to the anthropic environment of BH and reinforces its role as the main vector of L. infantum in the region. Instituto Oswaldo Cruz, Ministério da Saúde 2017-04 /pmc/articles/PMC5354617/ /pubmed/28327794 http://dx.doi.org/10.1590/0074-02760160438 Text en http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Articles Saraiva, Lara Leite, Camila Gonçalves Lima, Ana Cristina Vianna Mariano da Rocha de Carvalho, Luiz Otávio Alves Pereira, Agnes Antônia Sampaio Rugani, Jerônimo Marteleto Nunes Rego, Felipe Dutra Gontijo, Célia Maria Ferreira Andrade, José Dilermando Seasonality of sand flies (Diptera: Psychodidae) and Leishmania DNA detection in vector species in an area with endemic visceral leishmaniasis |
title | Seasonality of sand flies (Diptera: Psychodidae) and Leishmania DNA detection in vector species in an area with endemic visceral leishmaniasis |
title_full | Seasonality of sand flies (Diptera: Psychodidae) and Leishmania DNA detection in vector species in an area with endemic visceral leishmaniasis |
title_fullStr | Seasonality of sand flies (Diptera: Psychodidae) and Leishmania DNA detection in vector species in an area with endemic visceral leishmaniasis |
title_full_unstemmed | Seasonality of sand flies (Diptera: Psychodidae) and Leishmania DNA detection in vector species in an area with endemic visceral leishmaniasis |
title_short | Seasonality of sand flies (Diptera: Psychodidae) and Leishmania DNA detection in vector species in an area with endemic visceral leishmaniasis |
title_sort | seasonality of sand flies (diptera: psychodidae) and leishmania dna detection in vector species in an area with endemic visceral leishmaniasis |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5354617/ https://www.ncbi.nlm.nih.gov/pubmed/28327794 http://dx.doi.org/10.1590/0074-02760160438 |
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