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Homeobox A11 hypermethylation indicates unfavorable prognosis in breast cancer
Homeobox A11 (HOXA11) is one of the hypermethylated genes in breast cancer and its function in breast tumorigenesis remains elusive. In this study, we analyzed the methylation status of HOXA11 in 264 paired breast cancer and normal tissue as well as in matched serum samples by MethyLight assay. Furt...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5354771/ https://www.ncbi.nlm.nih.gov/pubmed/28038461 http://dx.doi.org/10.18632/oncotarget.14216 |
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author | Xia, Bingshu Shan, Ming Wang, Ji Zhong, Zhenbin Geng, Jingshu He, Xiaohui Vu, Tung Zhang, Dekai Pang, Da |
author_facet | Xia, Bingshu Shan, Ming Wang, Ji Zhong, Zhenbin Geng, Jingshu He, Xiaohui Vu, Tung Zhang, Dekai Pang, Da |
author_sort | Xia, Bingshu |
collection | PubMed |
description | Homeobox A11 (HOXA11) is one of the hypermethylated genes in breast cancer and its function in breast tumorigenesis remains elusive. In this study, we analyzed the methylation status of HOXA11 in 264 paired breast cancer and normal tissue as well as in matched serum samples by MethyLight assay. Further, the function of HOXA11 in breast tumorigenesis was analyzed by cell proliferation and migration assays. We found that HOXA11 was hypermethylated in cancer tissues (45.08%), especially in invasive ductal carcinomas (P<0.001), patients with a family history of cancer (P=0.033), cases with metastatic lymph nodes (P=0.004) and P53 positive group (P=0.017). Kaplan-Meier survival analysis and Cox regression analysis revealed that HOXA11 hypermethylation is an independent predictor of poor outcomes. The over expression of HOXA11 suppressed cell growth in MDA-MB-231, MCF7, SKBR3 and BT474 cells. In conclusion, the hypermethylation of HOXA11 is an independent prognostic biomarker in breast cancer. Additionally, HOXA11 can be a potential tumor suppressor. |
format | Online Article Text |
id | pubmed-5354771 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-53547712017-04-14 Homeobox A11 hypermethylation indicates unfavorable prognosis in breast cancer Xia, Bingshu Shan, Ming Wang, Ji Zhong, Zhenbin Geng, Jingshu He, Xiaohui Vu, Tung Zhang, Dekai Pang, Da Oncotarget Research Paper Homeobox A11 (HOXA11) is one of the hypermethylated genes in breast cancer and its function in breast tumorigenesis remains elusive. In this study, we analyzed the methylation status of HOXA11 in 264 paired breast cancer and normal tissue as well as in matched serum samples by MethyLight assay. Further, the function of HOXA11 in breast tumorigenesis was analyzed by cell proliferation and migration assays. We found that HOXA11 was hypermethylated in cancer tissues (45.08%), especially in invasive ductal carcinomas (P<0.001), patients with a family history of cancer (P=0.033), cases with metastatic lymph nodes (P=0.004) and P53 positive group (P=0.017). Kaplan-Meier survival analysis and Cox regression analysis revealed that HOXA11 hypermethylation is an independent predictor of poor outcomes. The over expression of HOXA11 suppressed cell growth in MDA-MB-231, MCF7, SKBR3 and BT474 cells. In conclusion, the hypermethylation of HOXA11 is an independent prognostic biomarker in breast cancer. Additionally, HOXA11 can be a potential tumor suppressor. Impact Journals LLC 2016-12-25 /pmc/articles/PMC5354771/ /pubmed/28038461 http://dx.doi.org/10.18632/oncotarget.14216 Text en Copyright: © 2017 Xia et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Xia, Bingshu Shan, Ming Wang, Ji Zhong, Zhenbin Geng, Jingshu He, Xiaohui Vu, Tung Zhang, Dekai Pang, Da Homeobox A11 hypermethylation indicates unfavorable prognosis in breast cancer |
title | Homeobox A11 hypermethylation indicates unfavorable prognosis in breast cancer |
title_full | Homeobox A11 hypermethylation indicates unfavorable prognosis in breast cancer |
title_fullStr | Homeobox A11 hypermethylation indicates unfavorable prognosis in breast cancer |
title_full_unstemmed | Homeobox A11 hypermethylation indicates unfavorable prognosis in breast cancer |
title_short | Homeobox A11 hypermethylation indicates unfavorable prognosis in breast cancer |
title_sort | homeobox a11 hypermethylation indicates unfavorable prognosis in breast cancer |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5354771/ https://www.ncbi.nlm.nih.gov/pubmed/28038461 http://dx.doi.org/10.18632/oncotarget.14216 |
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