MERS-CoV virus-like particles produced in insect cells induce specific humoural and cellular imminity in rhesus macaques

Middle East respiratory syndrome coronavirus (MERS-CoV) causes severe respiratory disease in humans with a case fatality rate of over 39%, and poses a considerable threat to public health. A lack of approved vaccine or drugs currently constitutes a roadblock in controlling disease outbreak and sprea...

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Detalles Bibliográficos
Autores principales: Wang, Chong, Zheng, Xuexing, Gai, Weiwei, Zhao, Yongkun, Wang, Hualei, Wang, Haijun, Feng, Na, Chi, Hang, Qiu, Boning, Li, Nan, Wang, Tiecheng, Gao, Yuwei, Yang, Songtao, Xia, Xianzhu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2016
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5355045/
https://www.ncbi.nlm.nih.gov/pubmed/27050368
http://dx.doi.org/10.18632/oncotarget.8475
Descripción
Sumario:Middle East respiratory syndrome coronavirus (MERS-CoV) causes severe respiratory disease in humans with a case fatality rate of over 39%, and poses a considerable threat to public health. A lack of approved vaccine or drugs currently constitutes a roadblock in controlling disease outbreak and spread. In this study, we generated MERS-CoV VLPs using the baculovirus expression system. Electron microscopy and immunoelectron microscopy results demonstrate that MERS-CoV VLPs are structurally similar to the native virus. Rhesus macaques inoculated with MERS-CoV VLPs and Alum adjuvant induced virus-neutralizing antibodies titers up to 1:40 and induced specific IgG antibodies against the receptor binding domain (RBD), with endpoint titers reaching 1:1,280. MERS-CoV VLPs also elicited T-helper 1 cell (Th1)-mediated immunity, as measured by ELISpot. These data demonstrate that MERS-CoV VLPs have excellent immunogenicity in rhesus macaques, and represent a promising vaccine candidate.

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