Cargando…
MERS-CoV virus-like particles produced in insect cells induce specific humoural and cellular imminity in rhesus macaques
Middle East respiratory syndrome coronavirus (MERS-CoV) causes severe respiratory disease in humans with a case fatality rate of over 39%, and poses a considerable threat to public health. A lack of approved vaccine or drugs currently constitutes a roadblock in controlling disease outbreak and sprea...
Autores principales: | , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5355045/ https://www.ncbi.nlm.nih.gov/pubmed/27050368 http://dx.doi.org/10.18632/oncotarget.8475 |
_version_ | 1782515449590710272 |
---|---|
author | Wang, Chong Zheng, Xuexing Gai, Weiwei Zhao, Yongkun Wang, Hualei Wang, Haijun Feng, Na Chi, Hang Qiu, Boning Li, Nan Wang, Tiecheng Gao, Yuwei Yang, Songtao Xia, Xianzhu |
author_facet | Wang, Chong Zheng, Xuexing Gai, Weiwei Zhao, Yongkun Wang, Hualei Wang, Haijun Feng, Na Chi, Hang Qiu, Boning Li, Nan Wang, Tiecheng Gao, Yuwei Yang, Songtao Xia, Xianzhu |
author_sort | Wang, Chong |
collection | PubMed |
description | Middle East respiratory syndrome coronavirus (MERS-CoV) causes severe respiratory disease in humans with a case fatality rate of over 39%, and poses a considerable threat to public health. A lack of approved vaccine or drugs currently constitutes a roadblock in controlling disease outbreak and spread. In this study, we generated MERS-CoV VLPs using the baculovirus expression system. Electron microscopy and immunoelectron microscopy results demonstrate that MERS-CoV VLPs are structurally similar to the native virus. Rhesus macaques inoculated with MERS-CoV VLPs and Alum adjuvant induced virus-neutralizing antibodies titers up to 1:40 and induced specific IgG antibodies against the receptor binding domain (RBD), with endpoint titers reaching 1:1,280. MERS-CoV VLPs also elicited T-helper 1 cell (Th1)-mediated immunity, as measured by ELISpot. These data demonstrate that MERS-CoV VLPs have excellent immunogenicity in rhesus macaques, and represent a promising vaccine candidate. |
format | Online Article Text |
id | pubmed-5355045 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-53550452017-04-15 MERS-CoV virus-like particles produced in insect cells induce specific humoural and cellular imminity in rhesus macaques Wang, Chong Zheng, Xuexing Gai, Weiwei Zhao, Yongkun Wang, Hualei Wang, Haijun Feng, Na Chi, Hang Qiu, Boning Li, Nan Wang, Tiecheng Gao, Yuwei Yang, Songtao Xia, Xianzhu Oncotarget Research Paper Middle East respiratory syndrome coronavirus (MERS-CoV) causes severe respiratory disease in humans with a case fatality rate of over 39%, and poses a considerable threat to public health. A lack of approved vaccine or drugs currently constitutes a roadblock in controlling disease outbreak and spread. In this study, we generated MERS-CoV VLPs using the baculovirus expression system. Electron microscopy and immunoelectron microscopy results demonstrate that MERS-CoV VLPs are structurally similar to the native virus. Rhesus macaques inoculated with MERS-CoV VLPs and Alum adjuvant induced virus-neutralizing antibodies titers up to 1:40 and induced specific IgG antibodies against the receptor binding domain (RBD), with endpoint titers reaching 1:1,280. MERS-CoV VLPs also elicited T-helper 1 cell (Th1)-mediated immunity, as measured by ELISpot. These data demonstrate that MERS-CoV VLPs have excellent immunogenicity in rhesus macaques, and represent a promising vaccine candidate. Impact Journals LLC 2016-03-30 /pmc/articles/PMC5355045/ /pubmed/27050368 http://dx.doi.org/10.18632/oncotarget.8475 Text en Copyright: © 2017 Wang et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Wang, Chong Zheng, Xuexing Gai, Weiwei Zhao, Yongkun Wang, Hualei Wang, Haijun Feng, Na Chi, Hang Qiu, Boning Li, Nan Wang, Tiecheng Gao, Yuwei Yang, Songtao Xia, Xianzhu MERS-CoV virus-like particles produced in insect cells induce specific humoural and cellular imminity in rhesus macaques |
title | MERS-CoV virus-like particles produced in insect cells induce specific humoural and cellular imminity in rhesus macaques |
title_full | MERS-CoV virus-like particles produced in insect cells induce specific humoural and cellular imminity in rhesus macaques |
title_fullStr | MERS-CoV virus-like particles produced in insect cells induce specific humoural and cellular imminity in rhesus macaques |
title_full_unstemmed | MERS-CoV virus-like particles produced in insect cells induce specific humoural and cellular imminity in rhesus macaques |
title_short | MERS-CoV virus-like particles produced in insect cells induce specific humoural and cellular imminity in rhesus macaques |
title_sort | mers-cov virus-like particles produced in insect cells induce specific humoural and cellular imminity in rhesus macaques |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5355045/ https://www.ncbi.nlm.nih.gov/pubmed/27050368 http://dx.doi.org/10.18632/oncotarget.8475 |
work_keys_str_mv | AT wangchong merscovviruslikeparticlesproducedininsectcellsinducespecifichumouralandcellularimminityinrhesusmacaques AT zhengxuexing merscovviruslikeparticlesproducedininsectcellsinducespecifichumouralandcellularimminityinrhesusmacaques AT gaiweiwei merscovviruslikeparticlesproducedininsectcellsinducespecifichumouralandcellularimminityinrhesusmacaques AT zhaoyongkun merscovviruslikeparticlesproducedininsectcellsinducespecifichumouralandcellularimminityinrhesusmacaques AT wanghualei merscovviruslikeparticlesproducedininsectcellsinducespecifichumouralandcellularimminityinrhesusmacaques AT wanghaijun merscovviruslikeparticlesproducedininsectcellsinducespecifichumouralandcellularimminityinrhesusmacaques AT fengna merscovviruslikeparticlesproducedininsectcellsinducespecifichumouralandcellularimminityinrhesusmacaques AT chihang merscovviruslikeparticlesproducedininsectcellsinducespecifichumouralandcellularimminityinrhesusmacaques AT qiuboning merscovviruslikeparticlesproducedininsectcellsinducespecifichumouralandcellularimminityinrhesusmacaques AT linan merscovviruslikeparticlesproducedininsectcellsinducespecifichumouralandcellularimminityinrhesusmacaques AT wangtiecheng merscovviruslikeparticlesproducedininsectcellsinducespecifichumouralandcellularimminityinrhesusmacaques AT gaoyuwei merscovviruslikeparticlesproducedininsectcellsinducespecifichumouralandcellularimminityinrhesusmacaques AT yangsongtao merscovviruslikeparticlesproducedininsectcellsinducespecifichumouralandcellularimminityinrhesusmacaques AT xiaxianzhu merscovviruslikeparticlesproducedininsectcellsinducespecifichumouralandcellularimminityinrhesusmacaques |