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Circulating exosomal microRNAs as prognostic biomarkers for non-small-cell lung cancer
Exosomal miRNAs are proposed as excellent candidate biomarkers for clinical applications. However, little is known about their potential roles as prognostic biomarkers in lung cancer. In this study, we explored the prognostic value of plasma exosomal microRNAs (miRNAs) for non-small-cell lung cancer...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5355076/ https://www.ncbi.nlm.nih.gov/pubmed/28055956 http://dx.doi.org/10.18632/oncotarget.14369 |
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author | Liu, Qingyun Yu, Zubin Yuan, Shuai Xie, Weijia Li, Chengying Hu, Zeyao Xiang, Ying Wu, Na Wu, Long Bai, Li Li, Yafei |
author_facet | Liu, Qingyun Yu, Zubin Yuan, Shuai Xie, Weijia Li, Chengying Hu, Zeyao Xiang, Ying Wu, Na Wu, Long Bai, Li Li, Yafei |
author_sort | Liu, Qingyun |
collection | PubMed |
description | Exosomal miRNAs are proposed as excellent candidate biomarkers for clinical applications. However, little is known about their potential roles as prognostic biomarkers in lung cancer. In this study, we explored the prognostic value of plasma exosomal microRNAs (miRNAs) for non-small-cell lung cancer (NSCLC). Using a quantitative polymerase chain reaction (qPCR) array panel, we analyzed 84 plasma exosomal miRNAs in 10 lung adenocarcinoma patients and 10 matched healthy controls. The qPCR array showed 30 aberrantly expressed exosomal miRNAs. Nine candidate miRNAs were selected based on differential expression and previous reports for further evaluating their prognostic roles in 196 NSCLC patients. Elevated levels of exosomal miR-23b-3p, miR-10b-5p and miR-21-5p were independently associated with poor overall survival (with hazard ratio [95% confidence interval]: 2.42 (1.45 - 4.04), P = 0.001; 2.22 (1.18 - 4.16), P = 0.013; 2.12 (1.28 - 3.49), P = 0.003, respectively). When compared to the clinical prognostic variables only model, adding the three exosomal miRNA signatures significantly improved survival predictive accuracy with an increase of time-dependent area under the receiver operating characteristic curve from 0.88 to 0.91 (P=0.015). Our results indicated that plasma exosomal miR-23b-3p, miR-10b-5p and miR-21-5p are promising non-invasive prognostic biomarkers of NSCLC. |
format | Online Article Text |
id | pubmed-5355076 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-53550762017-04-15 Circulating exosomal microRNAs as prognostic biomarkers for non-small-cell lung cancer Liu, Qingyun Yu, Zubin Yuan, Shuai Xie, Weijia Li, Chengying Hu, Zeyao Xiang, Ying Wu, Na Wu, Long Bai, Li Li, Yafei Oncotarget Research Paper Exosomal miRNAs are proposed as excellent candidate biomarkers for clinical applications. However, little is known about their potential roles as prognostic biomarkers in lung cancer. In this study, we explored the prognostic value of plasma exosomal microRNAs (miRNAs) for non-small-cell lung cancer (NSCLC). Using a quantitative polymerase chain reaction (qPCR) array panel, we analyzed 84 plasma exosomal miRNAs in 10 lung adenocarcinoma patients and 10 matched healthy controls. The qPCR array showed 30 aberrantly expressed exosomal miRNAs. Nine candidate miRNAs were selected based on differential expression and previous reports for further evaluating their prognostic roles in 196 NSCLC patients. Elevated levels of exosomal miR-23b-3p, miR-10b-5p and miR-21-5p were independently associated with poor overall survival (with hazard ratio [95% confidence interval]: 2.42 (1.45 - 4.04), P = 0.001; 2.22 (1.18 - 4.16), P = 0.013; 2.12 (1.28 - 3.49), P = 0.003, respectively). When compared to the clinical prognostic variables only model, adding the three exosomal miRNA signatures significantly improved survival predictive accuracy with an increase of time-dependent area under the receiver operating characteristic curve from 0.88 to 0.91 (P=0.015). Our results indicated that plasma exosomal miR-23b-3p, miR-10b-5p and miR-21-5p are promising non-invasive prognostic biomarkers of NSCLC. Impact Journals LLC 2016-12-30 /pmc/articles/PMC5355076/ /pubmed/28055956 http://dx.doi.org/10.18632/oncotarget.14369 Text en Copyright: © 2017 Liu et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Liu, Qingyun Yu, Zubin Yuan, Shuai Xie, Weijia Li, Chengying Hu, Zeyao Xiang, Ying Wu, Na Wu, Long Bai, Li Li, Yafei Circulating exosomal microRNAs as prognostic biomarkers for non-small-cell lung cancer |
title | Circulating exosomal microRNAs as prognostic biomarkers for non-small-cell lung cancer |
title_full | Circulating exosomal microRNAs as prognostic biomarkers for non-small-cell lung cancer |
title_fullStr | Circulating exosomal microRNAs as prognostic biomarkers for non-small-cell lung cancer |
title_full_unstemmed | Circulating exosomal microRNAs as prognostic biomarkers for non-small-cell lung cancer |
title_short | Circulating exosomal microRNAs as prognostic biomarkers for non-small-cell lung cancer |
title_sort | circulating exosomal micrornas as prognostic biomarkers for non-small-cell lung cancer |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5355076/ https://www.ncbi.nlm.nih.gov/pubmed/28055956 http://dx.doi.org/10.18632/oncotarget.14369 |
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