A neutralized human LMP1-IgG inhibits ENKTL growth by suppressing the JAK3/STAT3 signaling pathway

Latent membrane protein 1 (LMP1), which is associated with the development of different types of Epstein-Barr virus (EBV) related lymphoma, has been suggested to be an important oncoprotein. In this study, a human anti-LMP1 IgG antibody (LMP1-IgG) was constructed and characterized by ELISA, western...

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Autores principales: Mao, Yuan, Wang, Jun, Zhang, Mingzhi, Fan, Weifei, Tang, Qi, Xiong, Siping, Tang, Xiaojun, Xu, Juqing, Wang, Lin, Yang, Shu, Liu, Suyao, Xu, Li, Chen, Yan, Xu, Lin, Yin, Rong, Zhu, Jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2016
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5355237/
https://www.ncbi.nlm.nih.gov/pubmed/28009988
http://dx.doi.org/10.18632/oncotarget.14032
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author Mao, Yuan
Wang, Jun
Zhang, Mingzhi
Fan, Weifei
Tang, Qi
Xiong, Siping
Tang, Xiaojun
Xu, Juqing
Wang, Lin
Yang, Shu
Liu, Suyao
Xu, Li
Chen, Yan
Xu, Lin
Yin, Rong
Zhu, Jin
author_facet Mao, Yuan
Wang, Jun
Zhang, Mingzhi
Fan, Weifei
Tang, Qi
Xiong, Siping
Tang, Xiaojun
Xu, Juqing
Wang, Lin
Yang, Shu
Liu, Suyao
Xu, Li
Chen, Yan
Xu, Lin
Yin, Rong
Zhu, Jin
author_sort Mao, Yuan
collection PubMed
description Latent membrane protein 1 (LMP1), which is associated with the development of different types of Epstein-Barr virus (EBV) related lymphoma, has been suggested to be an important oncoprotein. In this study, a human anti-LMP1 IgG antibody (LMP1-IgG) was constructed and characterized by ELISA, western blotting (WB), affinity and immunohistochemistry (IHC) analyses. CCK-8, MTT, apoptosis assays, antibody-dependent cell-mediated cytotoxicity (ADCC) and CDC (complement-dependent cytotoxicity) assays were performed to evaluate the inhibitory effects of LMP1-IgG on extranodal nasal-type natural killer (NK)/T-cell lymphoma (ENKTL). Then, the influence of LMP1-IgG on the JAK/STAT signaling pathway was investigated. The results showed that the successfully constructed LMP1-IgG inhibited proliferation, induced apoptosis, and activated ADCC and CDC of ENKTL in a concentration- and time- dependent manner. Moreover, phosphorylation of JAK3 and STAT3 was inhibited by LMP1-IgG. Our data indicate that LMP1-IgG may provide a novel and promising therapeutic strategy for the treatment of LMP1-positive ENKTL.
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spelling pubmed-53552372017-04-26 A neutralized human LMP1-IgG inhibits ENKTL growth by suppressing the JAK3/STAT3 signaling pathway Mao, Yuan Wang, Jun Zhang, Mingzhi Fan, Weifei Tang, Qi Xiong, Siping Tang, Xiaojun Xu, Juqing Wang, Lin Yang, Shu Liu, Suyao Xu, Li Chen, Yan Xu, Lin Yin, Rong Zhu, Jin Oncotarget Research Paper Latent membrane protein 1 (LMP1), which is associated with the development of different types of Epstein-Barr virus (EBV) related lymphoma, has been suggested to be an important oncoprotein. In this study, a human anti-LMP1 IgG antibody (LMP1-IgG) was constructed and characterized by ELISA, western blotting (WB), affinity and immunohistochemistry (IHC) analyses. CCK-8, MTT, apoptosis assays, antibody-dependent cell-mediated cytotoxicity (ADCC) and CDC (complement-dependent cytotoxicity) assays were performed to evaluate the inhibitory effects of LMP1-IgG on extranodal nasal-type natural killer (NK)/T-cell lymphoma (ENKTL). Then, the influence of LMP1-IgG on the JAK/STAT signaling pathway was investigated. The results showed that the successfully constructed LMP1-IgG inhibited proliferation, induced apoptosis, and activated ADCC and CDC of ENKTL in a concentration- and time- dependent manner. Moreover, phosphorylation of JAK3 and STAT3 was inhibited by LMP1-IgG. Our data indicate that LMP1-IgG may provide a novel and promising therapeutic strategy for the treatment of LMP1-positive ENKTL. Impact Journals LLC 2016-12-20 /pmc/articles/PMC5355237/ /pubmed/28009988 http://dx.doi.org/10.18632/oncotarget.14032 Text en Copyright: © 2017 Mao et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Mao, Yuan
Wang, Jun
Zhang, Mingzhi
Fan, Weifei
Tang, Qi
Xiong, Siping
Tang, Xiaojun
Xu, Juqing
Wang, Lin
Yang, Shu
Liu, Suyao
Xu, Li
Chen, Yan
Xu, Lin
Yin, Rong
Zhu, Jin
A neutralized human LMP1-IgG inhibits ENKTL growth by suppressing the JAK3/STAT3 signaling pathway
title A neutralized human LMP1-IgG inhibits ENKTL growth by suppressing the JAK3/STAT3 signaling pathway
title_full A neutralized human LMP1-IgG inhibits ENKTL growth by suppressing the JAK3/STAT3 signaling pathway
title_fullStr A neutralized human LMP1-IgG inhibits ENKTL growth by suppressing the JAK3/STAT3 signaling pathway
title_full_unstemmed A neutralized human LMP1-IgG inhibits ENKTL growth by suppressing the JAK3/STAT3 signaling pathway
title_short A neutralized human LMP1-IgG inhibits ENKTL growth by suppressing the JAK3/STAT3 signaling pathway
title_sort neutralized human lmp1-igg inhibits enktl growth by suppressing the jak3/stat3 signaling pathway
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5355237/
https://www.ncbi.nlm.nih.gov/pubmed/28009988
http://dx.doi.org/10.18632/oncotarget.14032
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