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Prolonged Induction of miR-212/132 and REST Expression in Rat Striatum Following Cocaine Self-Administration

Chronic exposure to cocaine in vivo induces long-term synaptic plasticity associated with the brain’s circuitry that underlies development of repetitive and automatic behaviors called habits. In fact, prolonged drug consumption results in aberrant expression of protein-coding genes and small regulat...

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Autores principales: Sadakierska-Chudy, Anna, Frankowska, Małgorzata, Miszkiel, Joanna, Wydra, Karolina, Jastrzębska, Joanna, Filip, Małgorzata
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5355523/
https://www.ncbi.nlm.nih.gov/pubmed/26944283
http://dx.doi.org/10.1007/s12035-016-9817-2
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author Sadakierska-Chudy, Anna
Frankowska, Małgorzata
Miszkiel, Joanna
Wydra, Karolina
Jastrzębska, Joanna
Filip, Małgorzata
author_facet Sadakierska-Chudy, Anna
Frankowska, Małgorzata
Miszkiel, Joanna
Wydra, Karolina
Jastrzębska, Joanna
Filip, Małgorzata
author_sort Sadakierska-Chudy, Anna
collection PubMed
description Chronic exposure to cocaine in vivo induces long-term synaptic plasticity associated with the brain’s circuitry that underlies development of repetitive and automatic behaviors called habits. In fact, prolonged drug consumption results in aberrant expression of protein-coding genes and small regulatory RNAs, including miRNAs that are involved in synaptic plasticity and neuroadaptations. However, the mechanisms mediating cocaine use disorder are still not fully understood. The present study is designed to examine the expression of miR-124, miR-132, miR-134, and miR-212, as well as the levels of the Ago2, Pum2, and REST mRNAs and proteins implicated in their regulation. We applied rat cocaine self-administration (SA) and extinction training procedures with a yoked triad to assess the changes in the levels of four miRNAs and three protein-coding genes and corresponding proteins in the dorsal striatum. We demonstrated that elevated expression of mature miR-212 and miR-132 is long-lasting and persists in the drug-free period (till 10-day abstinence). Moreover, mRNA and protein of REST, a regulator of neuronal transcription, was raised selectively in cocaine self-administering rats and Ago2 transcript decreased after cocaine treatment. Unexpectedly, the expression level of Ago2 and Pum2 proteins changed only in the active cocaine-receiving animals. These results point out the important aspects of long-lasting alterations in microRNAs, genes, and protein expressions involved in the control of synaptic plasticity associated with reward and motivation learning related to cocaine addiction.
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spelling pubmed-53555232017-03-28 Prolonged Induction of miR-212/132 and REST Expression in Rat Striatum Following Cocaine Self-Administration Sadakierska-Chudy, Anna Frankowska, Małgorzata Miszkiel, Joanna Wydra, Karolina Jastrzębska, Joanna Filip, Małgorzata Mol Neurobiol Article Chronic exposure to cocaine in vivo induces long-term synaptic plasticity associated with the brain’s circuitry that underlies development of repetitive and automatic behaviors called habits. In fact, prolonged drug consumption results in aberrant expression of protein-coding genes and small regulatory RNAs, including miRNAs that are involved in synaptic plasticity and neuroadaptations. However, the mechanisms mediating cocaine use disorder are still not fully understood. The present study is designed to examine the expression of miR-124, miR-132, miR-134, and miR-212, as well as the levels of the Ago2, Pum2, and REST mRNAs and proteins implicated in their regulation. We applied rat cocaine self-administration (SA) and extinction training procedures with a yoked triad to assess the changes in the levels of four miRNAs and three protein-coding genes and corresponding proteins in the dorsal striatum. We demonstrated that elevated expression of mature miR-212 and miR-132 is long-lasting and persists in the drug-free period (till 10-day abstinence). Moreover, mRNA and protein of REST, a regulator of neuronal transcription, was raised selectively in cocaine self-administering rats and Ago2 transcript decreased after cocaine treatment. Unexpectedly, the expression level of Ago2 and Pum2 proteins changed only in the active cocaine-receiving animals. These results point out the important aspects of long-lasting alterations in microRNAs, genes, and protein expressions involved in the control of synaptic plasticity associated with reward and motivation learning related to cocaine addiction. Springer US 2016-03-05 2017 /pmc/articles/PMC5355523/ /pubmed/26944283 http://dx.doi.org/10.1007/s12035-016-9817-2 Text en © The Author(s) 2016 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Article
Sadakierska-Chudy, Anna
Frankowska, Małgorzata
Miszkiel, Joanna
Wydra, Karolina
Jastrzębska, Joanna
Filip, Małgorzata
Prolonged Induction of miR-212/132 and REST Expression in Rat Striatum Following Cocaine Self-Administration
title Prolonged Induction of miR-212/132 and REST Expression in Rat Striatum Following Cocaine Self-Administration
title_full Prolonged Induction of miR-212/132 and REST Expression in Rat Striatum Following Cocaine Self-Administration
title_fullStr Prolonged Induction of miR-212/132 and REST Expression in Rat Striatum Following Cocaine Self-Administration
title_full_unstemmed Prolonged Induction of miR-212/132 and REST Expression in Rat Striatum Following Cocaine Self-Administration
title_short Prolonged Induction of miR-212/132 and REST Expression in Rat Striatum Following Cocaine Self-Administration
title_sort prolonged induction of mir-212/132 and rest expression in rat striatum following cocaine self-administration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5355523/
https://www.ncbi.nlm.nih.gov/pubmed/26944283
http://dx.doi.org/10.1007/s12035-016-9817-2
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