Divergent Levels of Marker Chromosomes in an hiPSC-Based Model of Psychosis

In the process of generating presumably clonal human induced pluripotent stem cells (hiPSCs) from two carriers of a complex structural rearrangement, each having a psychotic disorder, we also serendipitously generated isogenic non-carrier control hiPSCs, finding that the rearrangement occurs as an e...

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Detalles Bibliográficos
Autores principales: TCW, Julia, Carvalho, Claudia M.B., Yuan, Bo, Gu, Shen, Altheimer, Alyssa N., McCarthy, Shane, Malhotra, Dheeraj, Sebat, Jonathan, Siegel, Arthur J., Rudolph, Uwe, Lupski, James R., Levy, Deborah L., Brennand, Kristen J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5355568/
https://www.ncbi.nlm.nih.gov/pubmed/28216146
http://dx.doi.org/10.1016/j.stemcr.2017.01.010
Descripción
Sumario:In the process of generating presumably clonal human induced pluripotent stem cells (hiPSCs) from two carriers of a complex structural rearrangement, each having a psychotic disorder, we also serendipitously generated isogenic non-carrier control hiPSCs, finding that the rearrangement occurs as an extrachromosomal marker (mar) element. All confirmed carrier hiPSCs and differentiated neural progenitor cell lines were found to be mosaic. We caution that mar elements may be difficult to functionally evaluate in hiPSC cultures using currently available methods, as it is difficult to distinguish cells with and without mar elements in live mosaic cultures.

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