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mRNA expression of delta-HER2 and its clinicopathological correlation in HER2-overexpressing breast cancer

Delta-human epidermal growth factor receptor 2 (HER2) is a splice variant of HER2, which lacks 16 amino acids in the extracellular domain. The aim of the present study was to elucidate the expression of delta-HER2 and its clinicopathological correlation in Japanese patients with HER2-overexpressing...

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Autores principales: Wada, Ryuichi, Yagihashi, Soroku, Naito, Zenya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5355676/
https://www.ncbi.nlm.nih.gov/pubmed/27840986
http://dx.doi.org/10.3892/mmr.2016.5892
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author Wada, Ryuichi
Yagihashi, Soroku
Naito, Zenya
author_facet Wada, Ryuichi
Yagihashi, Soroku
Naito, Zenya
author_sort Wada, Ryuichi
collection PubMed
description Delta-human epidermal growth factor receptor 2 (HER2) is a splice variant of HER2, which lacks 16 amino acids in the extracellular domain. The aim of the present study was to elucidate the expression of delta-HER2 and its clinicopathological correlation in Japanese patients with HER2-overexpressing breast cancer. A total of 40 cases of HER2-overexpressing breast cancer were investigated. The mRNA expression levels of wild type (wt)-HER2 and delta-HER2 were quantitated by quantitative polymerase chain reaction using RNA extracted from formalin-fixed paraffin-embedded specimens. In addition, the activated form of phosphorylated-SRC (pSRC) and Ki-67 were detected by immunostaining. The mRNA expression levels of wt-HER2 varied largely, and the mRNA levels of delta-HER2 were correlated with those of wt-HER2. Furthermore, the enhanced immunostaining of pSRC was detected in 15 cases. Unsupervised hierarchal clustering of wt-HER2, delta-HER2 and pSRC was used to separate the cases into three clusters. In the first cluster, the levels of wt-HER2 and delta-HER2 were low, and pSRC immunostaining was low. Two other clusters were characterized by moderately and highly increased levels of wt-HER2 and delta-HER2 mRNA expression and enhanced pSRC. The delta/wt ratio was higher in the first cluster. Positive lymph node status and recurrence were more frequent in the first cluster compared with those in the two other clusters. Furthermore, the delta/wt ratio was significantly increased in lymph node-positive and recurrent cases, compared with in lymph node-negative and non-recurrent cases. The present study demonstrated that delta-HER2 was expressed in Japanese patients with HER2-overexpressing breast cancer. High mRNA expression levels of delta-HER2 were associated with pSRC and increased proliferation of tumor cells. A poor prognosis may be predicted by the increase in delta/wt ratio in HER2-overexpressing breast cancer.
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spelling pubmed-53556762017-03-31 mRNA expression of delta-HER2 and its clinicopathological correlation in HER2-overexpressing breast cancer Wada, Ryuichi Yagihashi, Soroku Naito, Zenya Mol Med Rep Articles Delta-human epidermal growth factor receptor 2 (HER2) is a splice variant of HER2, which lacks 16 amino acids in the extracellular domain. The aim of the present study was to elucidate the expression of delta-HER2 and its clinicopathological correlation in Japanese patients with HER2-overexpressing breast cancer. A total of 40 cases of HER2-overexpressing breast cancer were investigated. The mRNA expression levels of wild type (wt)-HER2 and delta-HER2 were quantitated by quantitative polymerase chain reaction using RNA extracted from formalin-fixed paraffin-embedded specimens. In addition, the activated form of phosphorylated-SRC (pSRC) and Ki-67 were detected by immunostaining. The mRNA expression levels of wt-HER2 varied largely, and the mRNA levels of delta-HER2 were correlated with those of wt-HER2. Furthermore, the enhanced immunostaining of pSRC was detected in 15 cases. Unsupervised hierarchal clustering of wt-HER2, delta-HER2 and pSRC was used to separate the cases into three clusters. In the first cluster, the levels of wt-HER2 and delta-HER2 were low, and pSRC immunostaining was low. Two other clusters were characterized by moderately and highly increased levels of wt-HER2 and delta-HER2 mRNA expression and enhanced pSRC. The delta/wt ratio was higher in the first cluster. Positive lymph node status and recurrence were more frequent in the first cluster compared with those in the two other clusters. Furthermore, the delta/wt ratio was significantly increased in lymph node-positive and recurrent cases, compared with in lymph node-negative and non-recurrent cases. The present study demonstrated that delta-HER2 was expressed in Japanese patients with HER2-overexpressing breast cancer. High mRNA expression levels of delta-HER2 were associated with pSRC and increased proliferation of tumor cells. A poor prognosis may be predicted by the increase in delta/wt ratio in HER2-overexpressing breast cancer. D.A. Spandidos 2016-12 2016-10-26 /pmc/articles/PMC5355676/ /pubmed/27840986 http://dx.doi.org/10.3892/mmr.2016.5892 Text en Copyright: © Wada et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Wada, Ryuichi
Yagihashi, Soroku
Naito, Zenya
mRNA expression of delta-HER2 and its clinicopathological correlation in HER2-overexpressing breast cancer
title mRNA expression of delta-HER2 and its clinicopathological correlation in HER2-overexpressing breast cancer
title_full mRNA expression of delta-HER2 and its clinicopathological correlation in HER2-overexpressing breast cancer
title_fullStr mRNA expression of delta-HER2 and its clinicopathological correlation in HER2-overexpressing breast cancer
title_full_unstemmed mRNA expression of delta-HER2 and its clinicopathological correlation in HER2-overexpressing breast cancer
title_short mRNA expression of delta-HER2 and its clinicopathological correlation in HER2-overexpressing breast cancer
title_sort mrna expression of delta-her2 and its clinicopathological correlation in her2-overexpressing breast cancer
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5355676/
https://www.ncbi.nlm.nih.gov/pubmed/27840986
http://dx.doi.org/10.3892/mmr.2016.5892
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