Cargando…
Ganoderma lucidum polysaccharides protect fibroblasts against UVB-induced photoaging
Ganoderma lucidum has featured in traditional Chinese medicine for >1,000 years. Ganoderma polysaccharides (GL-PS), a major active ingredient in Ganoderma, confer immune regulation, antitumor effects and significant antioxidant effects. The aim of the present study was to investigate the efficacy...
Autores principales: | , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5355704/ https://www.ncbi.nlm.nih.gov/pubmed/27959406 http://dx.doi.org/10.3892/mmr.2016.6026 |
_version_ | 1782515639359897600 |
---|---|
author | Zeng, Qinghai Zhou, Fang Lei, Li Chen, Jing Lu, Jianyun Zhou, Jianda Cao, Ke Gao, Lihua Xia, Fang Ding, Shu Huang, Lihua Xiang, Hong Wang, Jingjing Xiao, Yangfan Xiao, Rong Huang, Jinhua |
author_facet | Zeng, Qinghai Zhou, Fang Lei, Li Chen, Jing Lu, Jianyun Zhou, Jianda Cao, Ke Gao, Lihua Xia, Fang Ding, Shu Huang, Lihua Xiang, Hong Wang, Jingjing Xiao, Yangfan Xiao, Rong Huang, Jinhua |
author_sort | Zeng, Qinghai |
collection | PubMed |
description | Ganoderma lucidum has featured in traditional Chinese medicine for >1,000 years. Ganoderma polysaccharides (GL-PS), a major active ingredient in Ganoderma, confer immune regulation, antitumor effects and significant antioxidant effects. The aim of the present study was to investigate the efficacy and mechanism of GL-PS-associated inhibition of ultraviolet B (UVB)-induced photoaging in human fibroblasts in vitro. Primary human skin fibroblasts were cultured, and a fibroblast photoaging model was built through exposure to UVB. Cell viability was measured by MTT assay. Aged cells were stained using a senescence-associated β-galactosidase staining (SA-β-gal) kit. ELISA kits were used to analyze matrix metalloproteinase (MMP) −1 and C-telopeptides of Type I collagen (CICP) protein levels in cellular supernatant. ROS levels were quantified by flow cytometry. Cells exposed to UVB had decreased cell viability, increased aged cells, decreased CICP protein expression, increased MMP-1 protein expression, and increased cellular ROS levels compared with non-exposed cells. However, cells exposed to UVB and treated with 10, 20 and 40 µg/ml GL-PS demonstrated increased cell viability, decreased aged cells, increased CICP protein expression, decreased MMP-1 protein expression, and decreased cellular ROS levels compared with UVB exposed/GL-PS untreated cells. These results demonstrate that GL-PS protects fibroblasts against photoaging by eliminating UVB-induced ROS. This finding indicates GL-PS treatment may serve as a novel strategy for antiphotoaging. |
format | Online Article Text |
id | pubmed-5355704 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-53557042017-03-31 Ganoderma lucidum polysaccharides protect fibroblasts against UVB-induced photoaging Zeng, Qinghai Zhou, Fang Lei, Li Chen, Jing Lu, Jianyun Zhou, Jianda Cao, Ke Gao, Lihua Xia, Fang Ding, Shu Huang, Lihua Xiang, Hong Wang, Jingjing Xiao, Yangfan Xiao, Rong Huang, Jinhua Mol Med Rep Articles Ganoderma lucidum has featured in traditional Chinese medicine for >1,000 years. Ganoderma polysaccharides (GL-PS), a major active ingredient in Ganoderma, confer immune regulation, antitumor effects and significant antioxidant effects. The aim of the present study was to investigate the efficacy and mechanism of GL-PS-associated inhibition of ultraviolet B (UVB)-induced photoaging in human fibroblasts in vitro. Primary human skin fibroblasts were cultured, and a fibroblast photoaging model was built through exposure to UVB. Cell viability was measured by MTT assay. Aged cells were stained using a senescence-associated β-galactosidase staining (SA-β-gal) kit. ELISA kits were used to analyze matrix metalloproteinase (MMP) −1 and C-telopeptides of Type I collagen (CICP) protein levels in cellular supernatant. ROS levels were quantified by flow cytometry. Cells exposed to UVB had decreased cell viability, increased aged cells, decreased CICP protein expression, increased MMP-1 protein expression, and increased cellular ROS levels compared with non-exposed cells. However, cells exposed to UVB and treated with 10, 20 and 40 µg/ml GL-PS demonstrated increased cell viability, decreased aged cells, increased CICP protein expression, decreased MMP-1 protein expression, and decreased cellular ROS levels compared with UVB exposed/GL-PS untreated cells. These results demonstrate that GL-PS protects fibroblasts against photoaging by eliminating UVB-induced ROS. This finding indicates GL-PS treatment may serve as a novel strategy for antiphotoaging. D.A. Spandidos 2017-01 2016-12-12 /pmc/articles/PMC5355704/ /pubmed/27959406 http://dx.doi.org/10.3892/mmr.2016.6026 Text en Copyright: © Zeng et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Zeng, Qinghai Zhou, Fang Lei, Li Chen, Jing Lu, Jianyun Zhou, Jianda Cao, Ke Gao, Lihua Xia, Fang Ding, Shu Huang, Lihua Xiang, Hong Wang, Jingjing Xiao, Yangfan Xiao, Rong Huang, Jinhua Ganoderma lucidum polysaccharides protect fibroblasts against UVB-induced photoaging |
title | Ganoderma lucidum polysaccharides protect fibroblasts against UVB-induced photoaging |
title_full | Ganoderma lucidum polysaccharides protect fibroblasts against UVB-induced photoaging |
title_fullStr | Ganoderma lucidum polysaccharides protect fibroblasts against UVB-induced photoaging |
title_full_unstemmed | Ganoderma lucidum polysaccharides protect fibroblasts against UVB-induced photoaging |
title_short | Ganoderma lucidum polysaccharides protect fibroblasts against UVB-induced photoaging |
title_sort | ganoderma lucidum polysaccharides protect fibroblasts against uvb-induced photoaging |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5355704/ https://www.ncbi.nlm.nih.gov/pubmed/27959406 http://dx.doi.org/10.3892/mmr.2016.6026 |
work_keys_str_mv | AT zengqinghai ganodermalucidumpolysaccharidesprotectfibroblastsagainstuvbinducedphotoaging AT zhoufang ganodermalucidumpolysaccharidesprotectfibroblastsagainstuvbinducedphotoaging AT leili ganodermalucidumpolysaccharidesprotectfibroblastsagainstuvbinducedphotoaging AT chenjing ganodermalucidumpolysaccharidesprotectfibroblastsagainstuvbinducedphotoaging AT lujianyun ganodermalucidumpolysaccharidesprotectfibroblastsagainstuvbinducedphotoaging AT zhoujianda ganodermalucidumpolysaccharidesprotectfibroblastsagainstuvbinducedphotoaging AT caoke ganodermalucidumpolysaccharidesprotectfibroblastsagainstuvbinducedphotoaging AT gaolihua ganodermalucidumpolysaccharidesprotectfibroblastsagainstuvbinducedphotoaging AT xiafang ganodermalucidumpolysaccharidesprotectfibroblastsagainstuvbinducedphotoaging AT dingshu ganodermalucidumpolysaccharidesprotectfibroblastsagainstuvbinducedphotoaging AT huanglihua ganodermalucidumpolysaccharidesprotectfibroblastsagainstuvbinducedphotoaging AT xianghong ganodermalucidumpolysaccharidesprotectfibroblastsagainstuvbinducedphotoaging AT wangjingjing ganodermalucidumpolysaccharidesprotectfibroblastsagainstuvbinducedphotoaging AT xiaoyangfan ganodermalucidumpolysaccharidesprotectfibroblastsagainstuvbinducedphotoaging AT xiaorong ganodermalucidumpolysaccharidesprotectfibroblastsagainstuvbinducedphotoaging AT huangjinhua ganodermalucidumpolysaccharidesprotectfibroblastsagainstuvbinducedphotoaging |