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Disruption of Autolysis in Bacillus subtilis using TiO(2) Nanoparticles

In contrast to many nanotoxicity studies where nanoparticles (NPs) are observed to be toxic or reduce viable cells in a population of bacteria, we observed that increasing concentration of TiO(2) NPs increased the cell survival of Bacillus subtilis in autolysis-inducing buffer by 0.5 to 5 orders of...

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Autores principales: McGivney, Eric, Han, Linchen, Avellan, Astrid, VanBriesen, Jeanne, Gregory, Kelvin B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5355886/
https://www.ncbi.nlm.nih.gov/pubmed/28303908
http://dx.doi.org/10.1038/srep44308
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author McGivney, Eric
Han, Linchen
Avellan, Astrid
VanBriesen, Jeanne
Gregory, Kelvin B.
author_facet McGivney, Eric
Han, Linchen
Avellan, Astrid
VanBriesen, Jeanne
Gregory, Kelvin B.
author_sort McGivney, Eric
collection PubMed
description In contrast to many nanotoxicity studies where nanoparticles (NPs) are observed to be toxic or reduce viable cells in a population of bacteria, we observed that increasing concentration of TiO(2) NPs increased the cell survival of Bacillus subtilis in autolysis-inducing buffer by 0.5 to 5 orders of magnitude over an 8 hour exposure. Molecular investigations revealed that TiO(2) NPs prevent or delay cell autolysis, an important survival and growth-regulating process in bacterial populations. Overall, the results suggest two potential mechanisms for the disruption of autolysis by TiO(2) NPs in a concentration dependent manner: (i) directly, through TiO(2) NP deposition on the cell wall, delaying the collapse of the protonmotive-force and preventing the onset of autolysis; and (ii) indirectly, through adsorption of autolysins on TiO(2) NP, limiting the activity of released autolysins and preventing further lytic activity. Enhanced darkfield microscopy coupled to hyperspectral analysis was used to map TiO(2) deposition on B. subtilis cell walls and released enzymes, supporting both mechanisms of autolysis interference. The disruption of autolysis in B. subtilis cultures by TiO(2) NPs suggests the mechanisms and kinetics of cell death may be influenced by nano-scale metal oxide materials, which are abundant in natural systems.
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spelling pubmed-53558862017-03-22 Disruption of Autolysis in Bacillus subtilis using TiO(2) Nanoparticles McGivney, Eric Han, Linchen Avellan, Astrid VanBriesen, Jeanne Gregory, Kelvin B. Sci Rep Article In contrast to many nanotoxicity studies where nanoparticles (NPs) are observed to be toxic or reduce viable cells in a population of bacteria, we observed that increasing concentration of TiO(2) NPs increased the cell survival of Bacillus subtilis in autolysis-inducing buffer by 0.5 to 5 orders of magnitude over an 8 hour exposure. Molecular investigations revealed that TiO(2) NPs prevent or delay cell autolysis, an important survival and growth-regulating process in bacterial populations. Overall, the results suggest two potential mechanisms for the disruption of autolysis by TiO(2) NPs in a concentration dependent manner: (i) directly, through TiO(2) NP deposition on the cell wall, delaying the collapse of the protonmotive-force and preventing the onset of autolysis; and (ii) indirectly, through adsorption of autolysins on TiO(2) NP, limiting the activity of released autolysins and preventing further lytic activity. Enhanced darkfield microscopy coupled to hyperspectral analysis was used to map TiO(2) deposition on B. subtilis cell walls and released enzymes, supporting both mechanisms of autolysis interference. The disruption of autolysis in B. subtilis cultures by TiO(2) NPs suggests the mechanisms and kinetics of cell death may be influenced by nano-scale metal oxide materials, which are abundant in natural systems. Nature Publishing Group 2017-03-17 /pmc/articles/PMC5355886/ /pubmed/28303908 http://dx.doi.org/10.1038/srep44308 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
McGivney, Eric
Han, Linchen
Avellan, Astrid
VanBriesen, Jeanne
Gregory, Kelvin B.
Disruption of Autolysis in Bacillus subtilis using TiO(2) Nanoparticles
title Disruption of Autolysis in Bacillus subtilis using TiO(2) Nanoparticles
title_full Disruption of Autolysis in Bacillus subtilis using TiO(2) Nanoparticles
title_fullStr Disruption of Autolysis in Bacillus subtilis using TiO(2) Nanoparticles
title_full_unstemmed Disruption of Autolysis in Bacillus subtilis using TiO(2) Nanoparticles
title_short Disruption of Autolysis in Bacillus subtilis using TiO(2) Nanoparticles
title_sort disruption of autolysis in bacillus subtilis using tio(2) nanoparticles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5355886/
https://www.ncbi.nlm.nih.gov/pubmed/28303908
http://dx.doi.org/10.1038/srep44308
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